A5087767533- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Spectroscopy and Quantum Chemical Studies
- Radiopharmaceutical Chemistry and Applications
- Synthesis and Biological Evaluation
- Electrochemical Analysis and Applications
- Photochemistry and Electron Transfer Studies
- Lipid Membrane Structure and Behavior
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Advanced Chemical Sensor Technologies
University of California, Berkeley
2019-2024
Berkeley College
2022-2024
University of Wisconsin–Madison
2023-2024
Abstract LAT assembly into a two-dimensional protein condensate is prominent feature of antigen discrimination by T cells. Here, we use single-molecule imaging techniques to resolve the spatial position and temporal duration each pMHC:TCR molecular binding event while simultaneously monitoring condensation at membrane. An individual sufficient trigger condensate, which self-limiting, neither its size nor lifetime correlated with originating event. Only probability forming related dwell time....
We report the hydrogen-bonding dynamics of water to a nitrile-functionalized and plasmonic electrode surface as function applied voltage. The surface-enhanced two-dimensional infrared spectra exhibit hydrogen-bonded non-hydrogen-bonded nitrile features in similar proportions, plus cross peaks between two. Isotopic dilution experiments show that arise predominantly from chemical exchange nitriles. rate depends upon voltage, with hydrogen bond nitriles breaking 2 3 times slower (>63 vs. 25...
Photosynthetic light harvesting requires efficient energy transfer within dynamic networks of light-harvesting complexes embedded phospholipid membranes. Artificial models are valuable tools for understanding the structural features underpinning absorption and chromophore arrays. Here, a method attaching protein-based model to planar, fluid supported lipid bilayer (SLB) is developed. The protein consists tobacco mosaic viral capsid proteins that gene-doubled create tandem dimer (dTMV)....
Antibody-derived T-cell receptor (TCR) agonists are commonly used to activate T cells. While antibodies can trigger TCRs regardless of clonotype, they bypass native cell signal integration mechanisms that rely on monovalent, membrane-associated, and relatively weakly-binding ligand in the context cellular adhesion. Commonly their derivatives bind much more strongly than peptide-MHC (pMHC) ligands cognate TCRs. Because dwell time is a critical parameter tightly correlates with physiological...
Antibody-derived T-cell receptor (TCR) agonists are commonly used to activate T cells. While antibodies can trigger TCRs regardless of clonotype, they bypass native cell signal integration mechanisms that rely on monovalent, membrane-associated, and relatively weakly binding ligand in the context cellular adhesion. Commonly their derivatives bind much more strongly than peptide major histocompatibility complex (pMHC) ligands cognate TCRs. Because dwell time is a critical parameter tightly...
SUMMARY LAT assembly into a two-dimensional protein condensate is prominent feature of antigen discrimination by T cells. Here, we use single-molecule imaging techniques to resolve the spatial position and temporal duration each pMHC:TCR molecular binding event while simultaneously monitoring condensation at membrane. An individual sufficient trigger condensate, which self-limiting, neither its size nor lifetime correlated with originating event. Only probability forming related dwell time....
The T cell receptor (TCR) is a complex molecular machine that directs the activation of cells, allowing immune system to fight pathogens and cancer cells. Despite decades investigation, mechanism TCR still controversial. One leading hypotheses allosteric model. This model posits binding pMHC at extracellular domain triggers dynamic change in transmembrane (TM) subunits, which leads signaling cytoplasmic side. We sought test this hypothesis by creating TM ligand for TCR. Previously we...
ABSTRACT Under physiological conditions, peptide-MHC (pMHC) molecules can trigger T-cell receptors (TCRs) as monovalent ligands that are sparsely distributed on the plasma membrane of an antigen-presenting cell. TCR also be activated by artificial clustering, such with pMHC tetramers or antibodies; however, these strategies circumvent many natural ligand discrimination mechanisms T cell and elicit non-physiological signaling activity. We have recently introduced a synthetic agonist composed...
Abstract The T cell receptor (TCR) is a complex molecular machine that directs the activation of cells, allowing immune system to fight pathogens and cancer cells. Despite decades investigation, mechanism TCR still controversial. One leading hypotheses allosteric model. This model posits binding pMHC at extracellular domain triggers dynamic change in transmembrane (TM) subunits, which leads signaling cytoplasmic side. We sought test this hypothesis by creating TM ligand for TCR. Previously...
Abstract Photosynthetic light harvesting requires efficient energy transfer within dynamic networks of complexes embedded phospholipid membranes. Artificial models are valuable tools for understanding the structural features underpinning absorption and chromophore arrays. Most artificial static or in solution phase, rather than a two-dimensional fluid environment as natural photosynthesis. We have developed method attaching protein-based model to supported lipid bilayer (SLB), which provides...