A5023148720- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Plant Molecular Biology Research
- Sirtuins and Resveratrol in Medicine
- Plant Stress Responses and Tolerance
- Amino Acid Enzymes and Metabolism
- Adenosine and Purinergic Signaling
- Soil and Environmental Studies
- Glioma Diagnosis and Treatment
- Click Chemistry and Applications
- Protein Degradation and Inhibitors
- RNA Interference and Gene Delivery
- Plant nutrient uptake and metabolism
- Trace Elements in Health
- Drug Transport and Resistance Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Folate and B Vitamins Research
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Cancer Genomics and Diagnostics
- Plant Reproductive Biology
University of California, Berkeley
2020-2023
University of North Carolina at Charlotte
2023
Princeton University
2017-2021
Czech Academy of Sciences, Institute of Experimental Botany
2013-2016
Cornell University
2013
Responses to drought, heat, and combined stress were compared in tobacco (Nicotiana tabacum L.) plants ectopically expressing the cytokinin oxidase/dehydrogenase CKX1 gene of Arabidopsis thaliana L. under control either predominantly root-expressed WRKY6 promoter or constitutive 35S promoter, wild type. WRKY6:CKX1 exhibited high CKX activity roots conditions. Under stress, was down-regulated concomitantly reduced degradation coincided with raised bioactive levels during early phase response,...
Chromatin landscape dictates remodeling Large, multicomponent molecular machines known as mammalian SWI/SNF (mSWI/SNF) chromatin-remodeling complexes play critical roles in governing the architecture of our genomes. These entities bind to chromatin (DNA assembled on proteins) inside nucleus and dictate which regions DNA, thus genes genome, are made accessible. It is that this process happens at right times for cells, because disruptions cause diseases such cancers neurodevelopmental...
Enhancer of Zeste Homolog 2 (EZH2) is the catalytic subunit Polycomb Repressor Complex (PRC2), enzyme that catalyzes monomethylation, dimethylation, and trimethylation lysine 27 on histone H3 (H3K27). Trimethylation at H3K27 (H3K27me3) associated with transcriptional silencing developmentally important genes. Intriguingly, H3K27me3 mutually exclusive H3K36 same tail. Disruptions in this cross-talk result aberrant H3K27/H3K36 methylation patterns altered profiles have been implicated...
One-carbon metabolism is an essential branch of cellular that intersects with epigenetic regulation. In this work, we show how formaldehyde (FA), a one-carbon unit derived from both endogenous sources and environmental exposure, regulates by inhibiting the biosynthesis
The AlkB family of nonheme Fe(II)/2-oxoglutarate-dependent oxygenases are essential regulators RNA epigenetics by serving as erasers one-carbon marks on with release formaldehyde (FA). Two major human members, FTO and ALKBH5, both act oxidative demethylases N6-methyladenosine (m6A) but furnish different products, N6-hydroxymethyladenosine (hm6A) adenosine (A), respectively. Here we identify foundational mechanistic differences between ALKBH5 that promote these distinct biochemical outcomes....
Significance Polycomb repressive complex 2 (PRC2) is a major epigenetic machinery that maintains transcriptionally silent heterochromatin in the nucleus and plays critical roles embryonic development oncogenesis. It generally thought PRC2 propagates histone marks by modifying neighboring nucleosomes strictly linear progression. However, behavior of on native-like chromatin substrates remains incompletely characterized, making precise mechanism PRC2-mediated maintenance elusive. Here we use...
In-depth chemical understanding of complex biological processes hinges upon the ability to systematically perturb individual systems. However, current approaches study impacts biologically relevant reactive small molecules involve bathing entire cell or isolated organelle with excess amounts, leading off-target effects. The resultant lack biochemical specificity has plagued our how electrophiles mediate signal transduction regulate responses that confer defense mechanisms cellular...
Cytokinins (CKs) are phytohormones regulating plant growth and development as well response to the environment. In order evaluate their function in heat stress (HS) responses, effect of CK elevation was determined during three types HS – targeted shoots, roots applied whole plant. The early (30min) longer term (3h) responses were followed at hormonal, transcriptomic proteomic levels Arabidopsis transformants with dexamethasone-inducible expression biosynthetic gene isopentenyltransferase...
Significance A subset of deadly pediatric brain tumors called diffuse intrinsic pontine gliomas express a mutant histone H3 with lysine-to-methionine substitution at position 27 (H3K27M), which has been shown to drive tumorigenesis in these cancers. While there is evidence that H3K27M inhibits the H3K27 methyltransferase known as polycomb repressive complex 2 (PRC2), precise mechanisms by acts remain matter debate. In this work, we used biochemical and cell assays characterize dependence...
Polycomb Repressive Complex 2 (PRC2) catalyzes mono-, di-, and trimethylation of lysine 27 on histone H3 (H3K27me1–3) to control expression genes important for differentiation maintenance cell identity. PRC2 activity is regulated by a number different inputs, including allosteric activation its product, H3K27me3. This positive feedback loop thought be the establishment large domains condensed heterochromatin. In addition other chromatin modifications, ancillary subunits PRC2, foremost...
<p>Abstract</p><p><br></p><p>The AlkB family of non-heme-Fe(II)/2-oxoglutarate(2OG)-dependent oxygenases are essential regulators RNA epigenetics by serving as erasers one-carbon marks on with release formaldehyde (FA). Two major human members, FTO and ALKBH5, both act oxidative demethylases N6 methyladenosine (m6A) but furnish different products, hydroxymethyladenosine (hm6A) adenosine (A), respectively. Here we identify foundational mechanistic...
Breast cancer represents the most frequently diagnosed malignancy among women with 10%–20% of all cases involving deadliest subtypes, triple-negative breast (TNBC).1Baranova A. Krasnoselskyi M. Starikov V. Kartashov S. Zhulkevych I. Vlasenko Oleshko K. Bilodid O. Sadchikova Vinnyk Y. Triple-negative cancer: current treatment strategies and factors negative prognosis.J. Med. Life. 2022; 15: 153-161https://doi.org/10.25122/jml-2021-0108Google Scholar The scarcity cell markers correlated TNBC...
ABSTRACT Polycomb repressive complex 2 (PRC2) maintains transcriptionally silent heterochromatin by installing and spreading histone methylation marks along nucleosome arrays. Despite extensive research, the mechanism which PRC2 engages with chromatin remains incompletely understood. Here we employ single-molecule force spectroscopy molecular dynamics simulations to dissect interactions of polynucleosome substrates. Our results reveal an unexpectedly diverse repertoire binding configurations...
Abstract The AlkB family of non-heme-Fe(II)/2-oxoglutarate(2OG)-dependent oxygenases are essential regulators RNA epigenetics by serving as erasers one-carbon marks on with release formaldehyde (FA). Two major human members, FTO and ALKBH5, both act oxidative demethylases N6 methyladenosine (m6A) but furnish different products, hydroxymethyladenosine (hm6A) adenosine (A), respectively. Here we identify foundational mechanistic differences between ALKBH5 that promote these distinct...