A5045030302- Plant Molecular Biology Research
- Seed Germination and Physiology
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Light effects on plants
- Allelopathy and phytotoxic interactions
- Plant Parasitism and Resistance
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Algal biology and biofuel production
- Plant Growth Enhancement Techniques
- Plant Physiology and Cultivation Studies
- Soybean genetics and cultivation
- Weed Control and Herbicide Applications
- Antioxidant Activity and Oxidative Stress
- Legume Nitrogen Fixing Symbiosis
- Arsenic contamination and mitigation
- Research in Cotton Cultivation
- RNA Research and Splicing
- Plant responses to elevated CO2
- Marine and coastal plant biology
- Biocrusts and Microbial Ecology
- Nitrogen and Sulfur Effects on Brassica
Palacký University Olomouc
2015-2025
Czech Academy of Sciences, Institute of Experimental Botany
2015-2025
University Hospital Olomouc
2015
Czech Academy of Sciences
2012-2015
Abstract The micropylar endosperm cap covering the radicle in mature seeds of most angiosperms acts as a constraint that regulates seed germination. Here, we report on comparative biology study with close Brassicaceae relatives Lepidium sativum and Arabidopsis thaliana showing ethylene biosynthesis signaling regulate germination by mechanism requires coordinated action cap. larger size allows direct tissue-specific biomechanical, biochemical, transcriptome analyses. We show promotes...
Responses to drought, heat, and combined stress were compared in tobacco (Nicotiana tabacum L.) plants ectopically expressing the cytokinin oxidase/dehydrogenase CKX1 gene of Arabidopsis thaliana L. under control either predominantly root-expressed WRKY6 promoter or constitutive 35S promoter, wild type. WRKY6:CKX1 exhibited high CKX activity roots conditions. Under stress, was down-regulated concomitantly reduced degradation coincided with raised bioactive levels during early phase response,...
Leaf senescence is an essential physiological process in plants that supports the recycling of nitrogen and other nutrients to support growth developing organs, including young leaves, seeds, fruits. Thus, regulation crucial for evolutionary success wild populations increasing yield crops. Here, we describe influence a NAC transcription factor, SlNAP2 (
Seed dormancy is an ecologically important adaptive trait in plants whereby germination repressed even under favorable conditions such as imbibition with water. In Arabidopsis and most plant species, absolutely requires unidentified seed coat germination-repressive activity constitutively higher abscisic acid (ABA) levels upon imbibition. The mechanisms underlying these processes their possible relationship are incompletely understood. We developed a “seed bedding” assay monitoring the...
Significance Mechanisms of plant seed dormancy evolved to delay germination a season favorable for seedling growth. Germination timing is an important adaptive early-life history trait which determines fitness in natural and agricultural ecosystems. The DELAY OF GERMINATION 1 ( DOG1 ) gene provides genetic variation dormancy, was the first dormancy-specific cloned, encodes protein unknown function. We show here that controls different species by setting optimal ambient temperature window...
Abstract Plant hormones, cytokinins (CKs), have been for a long time considered to be involved in plant responses stress. However, their exact roles processes linked stress signalization and acclimatization adverse environmental conditions are unknown. In this study, expression profiles of the entire gene families CK biosynthetic degradation genes maize (Zea mays) during development described. Transcript abundance particular is discussed relation levels different metabolites. Salt osmotic...
Phytochromes phyB and phyA mediate a remarkable developmental switch whereby, early upon seed imbibition, canopy light prevents phyB-dependent germination, whereas later on, it stimulates phyA-dependent germination. Using coat bedding assay where the growth of dissected embryos is monitored under influence endosperm, allowing combinatorial use mutant we show that specifically inactivates activity in endosperm to override signaling embryo. This interference involves abscisic acid (ABA)...
Summary The origin of phytohormones is poorly understood, and their physiological roles in microalgae remain elusive. Genome comparison photosynthetic autotrophic eukaryotes has revealed that the biosynthetic pathways abscisic acid ( ABA ) cytokinins CK s) emerged unicellular algae. While degradation mechanisms broadly algal lineages, complete vascular plant‐type conjugation prior to rise S treptophyta. In microalgae, a set proteins from canonical sensing signaling not essential, but...
miR156 is a conserved microRNA whose role and induction mechanisms under stress are poorly known. Strigolactones phytohormones needed in shoots for drought acclimation. They promote stomatal closure ABA-dependently independently; however, downstream effectors the former have not been identified. Linkage between strigolactones has reported. We compared ABA accumulation sensitivity as well performances of wt miR156-overexpressing (miR156-oe) tomato plants during drought. also quantified levels...
Endogenous cytokinins, auxins, and abscisic acid (ABA) were identified quantified in 11 red algae collected from the Brazilian coast. Field materials two isolates cultured laboratory extracted with various solvents buffers containing a mixture of appropriate internal standards, purified by solid‐phase extraction followed immunoaffinity chromatography, analyzed liquid chromatography–tandem mass spectrometry. Isoprenoid cytokinins (free conjugated forms isopentenyladenine [iP], cis ‐zeatin [ c...
Plant hormones become appropriate candidates for driving functional plant mycorrhization programs, including the processes that regulate formation of arbuscules in Arbuscular Mycorrhizal (AM) symbiosis. Here, we examine role played by ABA/GA interactions regulating AM tomato. We report differences ABA and GA metabolism between control mycorrhizal roots. Active synthesis catabolism occur GAs level increases as a consequence symbiosis-induced mechanism requires which turn is dependent on...
In eukaryotes, the decapping machinery is highly conserved and plays an essential role in controlling mRNA stability, a key step regulation of gene expression. Yet, shaping expression profiles response to environmental cues operating molecular mechanisms are poorly understood. Here, we provide genetic evidence that component machinery, LSM1-7 complex, critical plant tolerance abiotic stresses. Our results demonstrate that, depending on stress, complex from Arabidopsis thaliana interacts with...
Summary Salinity stress limits plant growth and has a major impact on agricultural productivity. Here, we identify NAC transcription factor SlTAF1 as regulator of salt tolerance in cultivated tomato ( Solanum lycopersicum ). While overexpression improves salinity compared with wild‐type, lowering expression causes stronger salinity‐induced damage. Under stress, shoots knockdown plants accumulate more toxic Na + ions, while overexpressors less accordance an altered the transporter genes...
Because carotenoids act as accessory pigments in photosynthesis, play a key photoprotective role and are of major nutritional importance, carotenogenesis has been target for crop improvement. Although important precursors phytohormones, previous genetic manipulations reported little if any effects on biomass production plant development, but resulted specific modifications carotenoid content. Unexpectedly, the expression carrot lycopene β-cyclase (DcLCYB1) Nicotiana tabacum cv. Xanthi not...
Myrica gale L. (sweet gale) fruit leachate contains myrigalone A (MyA), a rare C-methylated dihydrochalcone and putative allelochemical, which is known to be phytotoxin impeding seedling growth. We found that MyA inhibited Lepidium sativum seed germination in dose-dependent manner. did not affect testa rupture, but endosperm rupture the transition subsequent micropylar cap (CAP) weakening increase growth potential of radical/hypocotyl region (RAD) embryo, both being key processes required...
Abstract Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles photoprotection and antioxidative processes. β-Carotene is generated from lycopene by β-cyclase (LCYB). Previously, we demonstrated introduction Daucus carota (carrot) DcLCYB1 gene into tobacco (cv. Xanthi) resulted increased levels abscisic acid (ABA) especially gibberellins (GAs), resulting plant yield. In order to understand this phenomenon prior exporting genetic...
The Arabidopsis thaliana aldehyde oxidase 3 (AAO3) catalyzes the oxidation of abscisic (ABal) to acid (ABA). Besides ABal, plants generate other aldehydes that can be toxic above a certain threshold. AAO3 knockout mutants (aao3) exhibited earlier senescence but equivalent relative water content compared with wild-type (WT) during normal growth or upon application UV-C irradiation. Aldehyde profiling in leaves 24-day-old revealed higher accumulation acrolein, crotonaldehyde, 3Z-hexenal,...
Perennial para- and endo-dormancy are seasonally separate phenomena. Whereas para-dormancy is the suppression of axillary buds (AXBs) by a growing shoot, short-day elicited arrest terminal AXBs. In hybrid aspen (Populus tremula x P. tremuloides) compromising apex releases para-dormancy, whereas requires chilling. ABA GA implicated in both To untangle their roles, we blocked biosynthesis with fluridone (FD), which significantly reduced levels, downregulated GA-deactivation genes, upregulated...
Dormancy and heteromorphism are innate seed properties that control germination timing through adaptation to the prevailing environment. The degree of variation in dormancy depth within a population differs considerably depending on genotype maternal is therefore key trait annual weeds time seedling emergence across seasons. Seed heteromorphism, production distinct morphs (in color, mass or other morphological characteristics) same individual plant, considered be bet-hedging strategy...