A5017637521- Plant Molecular Biology Research
- Polysaccharides and Plant Cell Walls
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Tree Root and Stability Studies
- Plant Surface Properties and Treatments
- Advanced Cellulose Research Studies
- RNA and protein synthesis mechanisms
- Autophagy in Disease and Therapy
- Plant Parasitism and Resistance
- RNA modifications and cancer
- Plant biochemistry and biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Lipid metabolism and biosynthesis
- Plant Gene Expression Analysis
- Mitochondrial Function and Pathology
- Microtubule and mitosis dynamics
- Leaf Properties and Growth Measurement
- RNA Research and Splicing
- Soybean genetics and cultivation
- Plant Physiology and Cultivation Studies
- Postharvest Quality and Shelf Life Management
- Cellular Mechanics and Interactions
Max Planck Institute of Molecular Plant Physiology
2013-2025
Institut national de recherche en informatique et en automatique
2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2023
Centre National de la Recherche Scientifique
2023
California Institute of Technology
2014-2016
Pasadena City College
2016
Sainsbury Laboratory
2013-2014
University of Cambridge
2013-2014
Max Planck Society
2008-2012
Although it is a central question in biology, how cell shape controls intracellular dynamics largely remains an open question. Here, we show that the of Arabidopsis pavement cells creates stress pattern microtubule orientation, which then guides wall reinforcement. Live-imaging, combined with modeling mechanics, shows microtubules align along maximal tensile direction within cells, and atomic force microscopy demonstrates this leads to reinforcement parallel microtubules. This feedback loop...
In plants, regulation of cellulose synthesis is fundamental for morphogenesis and plant growth. Cellulose synthesized at the plasma membrane, orientation guided by cortical microtubules; however, guiding mechanism currently unknown. We show that conditional root elongation pom2 mutants are impaired in cell elongation, fertility, microtubule-related functions. Map-based cloning POM-POM2 locus revealed it allelic to CELLULOSE SYNTHASE INTERACTING1 (CSI1). Fluorescently tagged POM2/CSI1s...
Cellulose synthase-interactive protein 1 (CSI1) was identified in a two-hybrid screen for proteins that interact with cellulose synthase (CESA) isoforms involved primary plant cell wall synthesis. CSI1 encodes 2,150-amino acid contains 10 predicted Armadillo repeats and C2 domain. Mutations cause defective elongation hypocotyls roots reduce content. is associated CESA complexes, csi1 mutants affect the distribution movement of complexes plasma membrane.
The actin and microtubule cytoskeletons regulate cell shape across phyla, from bacteria to metazoans. In organisms with walls, the wall acts as a primary constraint of shape, generation specific depends on cytoskeletal organization for deposition and/or expansion. higher plants, cortical microtubules help organize construction by positioning delivery cellulose synthase (CesA) complexes guiding their trajectories orient newly synthesized microfibrils. cytoskeleton is required normal...
Plant cells are encased by a cellulose-containing wall that is essential for plant morphogenesis. Cellulose consists of β-1,4-linked glucan chains assembled into paracrystalline microfibrils synthesized plasma membrane–located cellulose synthase (CESA) complexes. Associations with hemicelluloses important microfibril spacing and maintaining cell tensile strength. Several components associated synthesis have been identified; however, the biological functions many them remain elusive. We show...
Abstract In eukaryotic cells, the actin and microtubule (MT) cytoskeletal networks are dynamic structures that organize intracellular processes facilitate their rapid reorganization. plant filaments (AFs) MTs essential for cell growth morphogenesis. However, interactions between these two components in live cells have not been explored. Here, we use spinning-disc confocal microscopy to dissect interaction cooperation cortical AFs Arabidopsis thaliana, utilizing fluorescent reporter...
Cellulose biosynthesis in plant secondary cell walls forms the basis of vascular development land plants, with xylem tissues constituting vast majority terrestrial biomass. We used lines that contained an inducible master transcription factor controlling fate to quantitatively image fluorescently tagged cellulose synthase enzymes during deposition living protoxylem cells. The formation wall thickenings was associated a redistribution and enrichment CESA7-containing complexes (CSCs) into...
An epidermis control of plant shoot stem cells can explain the scaling and position niche expression domains.
In nature, plants are constantly exposed to many transient, but recurring, stresses. Thus, complete their life cycles, require a dynamic balance between capacities recover following cessation of stress and maintenance memory. Recently, we uncovered new functional role for macroautophagy/autophagy in regulating recovery from heat (HS) resetting cellular memory HS Arabidopsis thaliana. Here, demonstrated that NBR1 (next BRCA1 gene 1) plays crucial as receptor selective autophagy during HS....
Plant organs can adopt a wide range of shapes, resulting from highly directional cell growth and divisions. We focus here on leaves leaf-like in Arabidopsis tomato, characterized by the formation thin, flat laminae. Combining experimental approaches with 3D mechanical modeling, we provide evidence that leaf shape depends cortical microtubule mediated cellulose deposition along main predicted stress orientations, particular, adaxial-abaxial axis internal walls. This behavior be explained...
Abstract Freezing triggers extracellular ice formation leading to cell dehydration and deformation during a freeze–thaw cycle. Many plant species increase their freezing tolerance exposure low, non‐freezing temperatures, process termed cold acclimation. In addition, mild temperatures after acclimation evokes further in (sub‐zero acclimation). Previous transcriptome proteome analyses indicate that wall remodelling may be particularly important for sub‐zero the present study, we used...
Mechanical stress influences cell- and tissue-scale processes across all kingdoms. It remains challenging to delineate how mechanical stress, originating at these different length scales, impacts cell tissue form. We combine growth tracking of cells, quantitative image analysis, as well molecular perturbations address this problem in pavement cells Arabidopsis thaliana cotyledon tissue. show that microtubule organization based on chemical signals cell-shape-derived varies during early stages...
Abstract Plants evolved efficient multifaceted acclimation strategies to cope with low temperatures. Chloroplasts respond temperature stimuli and participate in sensing acclimation. However, very little is known about the involvement of chloroplast genes their expression plant chilling tolerance. Here we systematically investigated cold tobacco seedlings over 2 days exposure temperatures by examining responses genome copy number, transcript accumulation translation, photosynthesis, cell...
Mechanical forces control development in plants and animals, acting as cues pattern formation the driving force of morphogenesis. In mammalian cells, molecular assemblies residing at interface cell membrane extracellular matrix play an important role perceiving transmitting external mechanical signals to trigger physiological responses. Similar processes occur plants, but there is little understanding mechanisms their genetic basis. Here, we show that number movement directions cellulose...
Postharvest physiological deterioration (PPD) reduces the availability and economic value of fresh produces, resulting in waste agricultural products becoming a worldwide problem. Therefore, many studies have been carried out at anatomical structural, biochemical levels molecular PPD produces to seek ways manage postharvest quality produce. The cell wall is outermost structure plant as such represents first barrier prevent external microorganisms other injuries. Many on crop storage organs...
Gene duplications generate new genes that can acquire similar but often diversified functions. Recent studies of gene coexpression networks have indicated that, not only genes, also pathways be multiplied and to perform related functions in different parts an organism. Identification such pathways, or modules, is needed expand our knowledge biological processes plants understand how evolve. However, systematic explorations modules remain scarce, no user-friendly platform identify them...
Abstract Glycolysis is one of the primordial pathways metabolism, playing a pivotal role in energy metabolism and biosynthesis. Glycolytic enzymes are known to form transient multi-enzyme assemblies. Here we examine wider protein-protein interactions plant glycolytic reveal moonlighting for specific mediating co-localization mitochondria chloroplasts. Knockout mutation phosphoglycerate mutase or enolase resulted significantly reduced association two organelles. We provide evidence that...
How organisms attain their specific shapes and modify growth patterns in response to environmental chemical signals has been the subject of many investigations. Plant cells are at high turgor pressure, surrounded by a rigid yet flexible cell wall, which is primary determinant plant morphogenesis. Cellulose microfibrils, synthesized plasma membrane-localized cellulose synthase complexes, major tension-bearing components wall that mediate directional growth. Despite advances understanding...
Abstract Plants are the tallest organisms on Earth; a feature sustained by solute-transporting xylem vessels in plant vasculature. The supported strong cell walls that assembled intricate patterns. Cortical microtubules direct wall deposition and need to rapidly re-organize during development. Here, we establish long-term live-cell imaging of single Arabidopsis cells undergoing proto-xylem trans -differentiation, resulting spiral patterns, understand microtubule re-organization. We find...