A5073236451- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Spaceflight effects on biology
- Monoclonal and Polyclonal Antibodies Research
- Force Microscopy Techniques and Applications
- Liver physiology and pathology
- Space Exploration and Technology
- Microtubule and mitosis dynamics
- Immune Response and Inflammation
- Platelet Disorders and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Planetary Science and Exploration
- Microfluidic and Bio-sensing Technologies
- Erythrocyte Function and Pathophysiology
- Blood properties and coagulation
- Pluripotent Stem Cells Research
- Polysaccharides and Plant Cell Walls
- Liver Disease Diagnosis and Treatment
- Plant Molecular Biology Research
- Biochemical and Structural Characterization
- Protease and Inhibitor Mechanisms
- Plant Reproductive Biology
- Microfluidic and Capillary Electrophoresis Applications
- Lipid Membrane Structure and Behavior
Institute of Mechanics
2016-2025
Chinese Academy of Sciences
2016-2025
University of Chinese Academy of Sciences
2016-2025
Hunan University
2023
State Key Laboratory of Chemobiosensing and Chemometrics
2023
Dartmouth College
2023
Columbia University
2023
Politecnico di Milano
2022
Lens Technology (China)
2021
National Physical Laboratory
2021
Replicate key structures and functions in a liver chip integrating shear flow primary hepatic cells.
Plant organs can adopt a wide range of shapes, resulting from highly directional cell growth and divisions. We focus here on leaves leaf-like in Arabidopsis tomato, characterized by the formation thin, flat laminae. Combining experimental approaches with 3D mechanical modeling, we provide evidence that leaf shape depends cortical microtubule mediated cellulose deposition along main predicted stress orientations, particular, adaxial-abaxial axis internal walls. This behavior be explained...
Lymphocyte function-associated antigen-1 (LFA-1) and macrophage-1 antigen (Mac-1) their counterreceptors such as intercellular cell adhesion molecules (ICAM-1 ICAM-2), junctional (JAM-A, JAM-C), receptors for advanced glycation end products (RAGE) are crucial promoting polymorphonuclear leukocyte (neutrophil, PMN) recruitment. The underlying mechanisms of ligand-specific bindings in this cascade remain incompletely known. We compared the dynamic force spectra various LFA-1/Mac-1-ligand bonds...
AIM: To study the viscoelastic properties of human hepatocytes and hepatocellular carcinoma (HCC) cells under cytoskeletal perturbation, to further adhesive mouse hepatoma (HTC) in different cell cycle. METHODS:Micropipette aspiration technique was adopted measure coefficients adhesion force collagen coated surface cells.Three kinds cytoskeleton perturbing agents, colchicines (Col), cytochalasin D (CD) vinblastine (VBL), were used treat HCC effects these treatment on investigated.The...
Surface presentation of adhesion receptors influences cell adhesion, although the mechanisms underlying these effects are not well understood. We used a micropipette frequency assay to quantify how molecular orientation and length on membrane affected two-dimensional kinetic rates interactions with surface ligands. Interactions P-selectin, E-selectin, CD16A their respective ligands or antibody were demonstrate such effects. Randomizing receptor lowering its ligand- antibody-binding domain...
Endothelial cells (ECs) are mechanosensitive undergoing morphological and functional changes in space. Ground-based study has provided a body of evidences about how ECs can respond to the effect simulated microgravity, however, these results need be confirmed by spaceflight experiments real microgravity. In this work, we cultured EA.hy926 on board SJ-10 Recoverable Scientific Satellite for 3 10 days, analyzed effects space microgravity ECs. Space suppressed glucose metabolism, modulated...
Macrophage-1 Ag (Mac-1) and lymphocyte function-associated Ag-1 (LFA-1), two β2 integrins expressed on neutrophils (PMNs), mediate PMN recruitment cascade by binding to intercellular adhesive molecule 1. Distinct functions of LFA-1-initiating slow rolling firm adhesion but Mac-1-mediating cell crawling are assumed be governed the differences in their affinities kinetic rates. In this study, we applied an frequency approach compare kinetics quiescent activated states using three molecular...
Article12 December 2019Open Access Source DataTransparent process Biomechanical stress regulates mammalian tooth replacement via the integrin β1-RUNX2-Wnt pathway Xiaoshan Wu Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School Stomatology, Beijing, China Department Oral Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, Search for more papers by this author Jinrong Hu Center Biomechanics Bioengineering,...
Abstract Atomic force microscopy (AFM) has been widely used to acquire surface topography upon different scanning modes and quantify mechanical properties of a cell using single-point ramp mode. However, these traditional measurements need massive curves originating from multiple points exclude the potential errors resulted limited factitious selections testing points, making time-consuming highly localized. PeakForce Quantitative NanoMechanics (PF QNM) is high-speed (faster by 3–4 order...
Extracellular matrix (ECM) rigidity has important effects on cell behaviors and increases sharply in liver fibrosis cirrhosis. Hepatic blood flow is essential maintaining hepatocytes' (HCs) functions. However, it still unclear how stiffness shear stresses orchestrate HC phenotype concert. A fibrotic three-dimensional (3-D) sinusoidal model constructed using a porous membrane sandwiched between two polydimethylsiloxane (PDMS) layers with respective channels. The HCs are cultured collagen gels...
Background & Aims: Partial hepatectomy (PHx)-induced liver regeneration causes the increase in relative blood flow rate within liver, which dilates hepatic sinusoids and applies mechanical stretch on sinusoidal endothelial cells (LSECs). Heparin-binding EGF-like growth factor (HB-EGF) is a crucial during regeneration. We aimed to investigate whether this dilation-induced promotes HB-EGF secretion LSECs what related molecular mechanism is. Approach Results: In vivo PHx, ex perfusion vitro...
The viscoelastic properties of both hepatocytes and hepatocellular carcinoma (HCC) cells were measured by means a micropipette aspiration technique. Experimental results analyzed with three-element standard linear solid model, in which an elastic element, K1, is parallel Maxwell element composed another K2, series viscous mu. Further, we investigated the relevance these two types to cytoskeleton structures treating three cytoskeletal perturbing agents, namely cytochalasin D (CD), colchicine...
Mechanics and surface microtopology of the molecular carrier influence cell adhesion, but mechanisms underlying these effects are not well understood. We used a micropipette adhesion frequency assay to quantify how stiffness affected two-dimensional kinetics interacting molecules on two apposing surfaces. Interactions P-selectin with glycoprotein ligand-1 (PSGL-1) were demonstrate such by presenting three systems: human red blood cells (RBCs), promyelocytic leukemia HL-60 cells, polystyrene...