A5014356088- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- Optical Imaging and Spectroscopy Techniques
- Advanced Biosensing Techniques and Applications
- Mosquito-borne diseases and control
- Photosynthetic Processes and Mechanisms
- Antimicrobial Peptides and Activities
- Spectroscopy and Quantum Chemical Studies
- Cell Image Analysis Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cellular Mechanics and Interactions
- Monoclonal and Polyclonal Antibodies Research
- Sphingolipid Metabolism and Signaling
- Bacterial biofilms and quorum sensing
- Receptor Mechanisms and Signaling
- Viral Infections and Vectors
- Analytical Chemistry and Sensors
- Immune Response and Inflammation
- Spectroscopy and Chemometric Analyses
- Wnt/β-catenin signaling in development and cancer
- Developmental Biology and Gene Regulation
- Cellular transport and secretion
- Zebrafish Biomedical Research Applications
- Photoreceptor and optogenetics research
- Aquaculture disease management and microbiota
National University of Singapore
2016-2025
Singapore-MIT Alliance for Research and Technology
2008-2018
Singapore Bioimaging Consortium
2016
International Centre of Biodynamics
2009
Okinawa Institute of Science and Technology Graduate University
2007
Genome Institute of Singapore
2006
École Polytechnique Fédérale de Lausanne
1999-2003
Stanford University
2002
Howard Hughes Medical Institute
2002
Much has been learned about the role of exofacial phosphatidylserine (PS) in apoptosis and blood clotting using annexin V. However, because annexins are impermeant unable to bind PS at low calcium concentration, they unsuitable for intracellular use. Thus little is known topology dynamics endomembranes normal cells. We used two new probes-green fluorescent protein (GFP)-LactC2, a genetically encoded biosensor, 1-palmitoyl-2-(dipyrrometheneboron...
Abstract Background Early events in vertebrate liver development have been the major focus previous studies, however, late of organogenesis remain poorly understood. Liver vasculogenesis vertebrates occurs through interaction endoderm-derived epithelium and mesoderm-derived endothelial cells (ECs). In zebrafish, although it has found that ECs are not required for budding, how when spatio-temporal pattern growth is coordinated with remains to be elucidated. Results To study process vivo , a...
Abstract Molecular diffusion in biological membranes is a determining factor cell signaling and function. In the past few decades, three main fluorescence spectroscopy techniques have emerged that are capable of measuring molecular artificial at very different concentration ranges spatial resolutions. The widely used methods recovery after photobleaching (FRAP) single‐particle tracking (SPT) can determine absolute coefficients high (>100 μm −2 ) low surface concentrations (single‐molecule...
The electronic properties of graphene can be modulated by charged lipid bilayer adsorbing on the surface. Biorecognition events which lead to changes in membrane integrity monitored electrically using an electrolyte-gated biomimetic membrane−graphene transistor. Here, we demonstrate that bactericidal activity antimicrobial peptides sensed based a complex interplay biomolecular doping and ionic screening effect.
The life sciences require new highly sensitive imaging tools, which allow the quantitative measurement of molecular parameters within a physiological three-dimensional (3D) environment. Therefore, we combined single plane illumination microscopy (SPIM) with camera based fluorescence correlation spectroscopy (FCS). SPIM-FCS provides contiguous particle number and diffusion coefficient images high spatial resolution in homo- heterogeneous 3D specimens live zebrafish embryos. Our recorded up to...
ABSTRACT Morphogen gradients provide essential positional information to gene networks through their spatially heterogeneous distribution, yet how they form is still hotly contested, with multiple models proposed for different systems. Here, we focus on the transcription factor Bicoid (Bcd), a morphogen that forms an exponential gradient across anterior-posterior (AP) axis of early Drosophila embryo. Using fluorescence correlation spectroscopy find there are spatial differences in Bcd...
The 5-hydroxytryptamine receptor of type 3 was investigated by fluorescence correlation spectroscopy (FCS). Binding constants fluorescently labeled ligands, the stoichiometry, and mass are readily accessible this technique, while duration measurement is on order seconds to minutes. antagonist 1,2,3,9-tetrahydro-3-[(5-methyl-1H-imidazol-4-yl)methyl]-9-(3-aminopropyl)-4H-carbazol-4-one (GR-H) with fluorophores rhodamine 6G, fluorescein, N-[7-nitrobenz-2-oxa-1,3-diazol-4-yl], cyanine dye Cy5....
A spatially resolved total internal reflection fluorescence correlation microscopy (TIR-FCM) system is constructed with an electron multiplying charge-coupled device (EMCCD) camera. The was used to determine diffusion coefficients of lipid molecules in a planar bilayer, and lipids epidermal growth factor receptor (EGFR) proteins on cell membranes Chinese Hamster Ovary (CHO) cells. evaluation the "cross talk" between neighboring pixels suggests that higher degree multiplexing can be achieved...
Imaging fluorescence correlation spectroscopy (FCS) performed using array detectors has been successfully used to quantify the number, mobility, and organization of biomolecules in cells organisms. However, there have not any systematic studies on errors these estimates that are introduced due instrumental experimental factors. State-of-the-art still restricted number frames can be recorded per unit time, sensitivity noise characteristics, total realistically recorded. These limitations...
Single plane illumination microscopy based fluorescence correlation spectroscopy (SPIM-FCS) is a new method for imaging FCS in 3D samples, providing diffusion coefficients, transport, flow velocities and concentrations an mode.SPIM-FCS records functions over whole sample, which requires array detectors recording the signal.Several types of image sensors are suitable FCS.They differ properties such as effective area per pixel, quantum efficiency, noise level read-out speed.Here we compare...
Macromolecular crowding (MMC) is a biophysical effect that governs biochemical processes inside and outside of cells. Since standard cell culture media lack this effect, the physiological performance differentiated progenitor cells, including extracellular matrix (ECM) deposition, impaired in vitro. To bring back crowdedness to vitro systems, we have previously introduced carbohydrate-based macromolecules achieved marked improvements with mixed MMC terms ECM deposition differentiation...
Dengue virus serotype 2 (DENV2) alone undergoes structural expansion at 37 °C (associated with host entry), despite high sequence and homology among the four known serotypes. The basis for this differential across strains serotypes is unknown necessitates mapping of dynamics dengue whole viral particles to describe their coordinated motions conformational changes when exposed host-like environments. Here we capture intact two serotypes, DENV1 DENV2, by amide hydrogen/deuterium exchange mass...
Abstract Super-resolution microscopy and single molecule fluorescence spectroscopy require mutually exclusive experimental strategies optimizing either temporal or spatial resolution. To achieve both, we implement a GPU-supported, camera-based measurement strategy that highly resolves structures (~100 nm), dynamics (~2 ms), molecular brightness from the exact same data set. Simultaneous super-resolution of details leads to an improved precision in estimating diffusion coefficient actin...