A5009604211- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Plant Parasitism and Resistance
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Light effects on plants
- Plant and animal studies
- Allelopathy and phytotoxic interactions
- Plant Virus Research Studies
- Polysaccharides and Plant Cell Walls
- Legume Nitrogen Fixing Symbiosis
- Plant responses to water stress
- Seed Germination and Physiology
- Plant Gene Expression Analysis
- Insect-Plant Interactions and Control
- Plant Physiology and Cultivation Studies
- Marine and coastal plant biology
- Plant Growth Enhancement Techniques
- Agronomic Practices and Intercropping Systems
- Plant and Biological Electrophysiology Studies
- Education, Psychology, and Social Research
- Fungal Biology and Applications
Palacký University Olomouc
2016-2025
Czech Academy of Sciences, Institute of Experimental Botany
2016-2025
Swedish University of Agricultural Sciences
2014-2024
Umeå Plant Science Centre
2014-2024
Czech Academy of Sciences
2005-2022
Charles University
2019-2022
Institut des Sciences des Plantes de Montpellier
2020
University of Utah
2009-2019
National Institute of Mental Health
2019
Centre of the Region Haná for Biotechnical and Agricultural Research
2016-2018
We used loss-of-function mutants to study three Arabidopsis thaliana sensor histidine kinases, AHK2, AHK3, and CRE1/AHK4, known be cytokinin receptors. Mutant seeds had more rapid germination, reduced requirement for light, decreased far-red light sensitivity, unraveling functions in seed germination control. Triple mutant were than twice as large wild-type seeds. Genetic analysis indicated a cytokinin-dependent endospermal and/or maternal control of embryo size. Unchanged red sensitivity...
Optimizing root system architecture can overcome yield limitations in crop plants caused by water or nutrient shortages. Classic breeding approaches are difficult because the trait is governed many genes and to score. We generated transgenic Arabidopsis thaliana tobacco (Nicotiana tabacum) with enhanced root-specific degradation of hormone cytokinin, a negative regulator growth. These form larger system, whereas growth development shoot similar. Elongation primary root, branching, biomass...
Vegetative shoot-based propagation of plants, including mass elite genotypes, is dependent on the development shoot-borne roots, which are also called adventitious roots. Multiple endogenous and environmental factors control complex process rooting. In past few years, we have shown that auxin response ARF6 ARF8, targets microRNA miR167, positive regulators rooting, whereas ARF17, a target miR160, negative regulator. We showed these genes overlapping expression profiles during rooting they...
Phytohormones are physiologically important small molecules that play essential roles in intricate signaling networks regulate diverse processes plants. We present a method for the simultaneous targeted profiling of 101 phytohormone-related analytes from minute amounts fresh plant material (less than 20 mg). Rapid and nonselective extraction, fast one-step sample purification, extremely sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry enable concurrent...
The effects of auxins on plant growth and development have been known for more than 100 years, yet our understanding how plants synthesize this essential hormone is still fragmentary at best. Gene loss- gain-of-function studies conclusively implicated three gene families, CYTOCHROME P450 79B2/B3 (CYP79B2/B3), YUCCA (YUC), TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS1/TRYPTOPHAN AMINOTRANSFERASE-RELATED (TAA1/TAR), in the production reference Arabidopsis thaliana. Each these families believed...
Coordination of cell division and pattern formation is central to tissue organ development, particularly in plants where walls prevent migration. Auxin cytokinin are both critical for patterning, but it unknown how these hormones converge upon development. We identify a genetic network that reinforces an early embryonic bias auxin distribution create local, nonresponding source within the root vascular tissue. Experimental theoretical evidence shows cells act as organizer by positioning...
Cytokinins (CKs) are plant hormones affecting numerous developmental processes. Zeatin and its derivatives the most important group of isoprenoid CKs. occurs as two isomers: while trans-zeatin (transZ) was found to be a bioactive substance, cis-zeatin (cisZ) reported have weak biological impact. Even though cisZ abundant in various materials their role is still unknown. The comprehensive screen land plants presented here suggests that cisZ-type CKs occur ubiquitously kingdom but abundance...
The plant hormone auxin is believed to influence almost every aspect of growth and development. Auxin transport, biosynthesis degradation combine form gradients the that a range key developmental environmental response processes. There abundant genetic evidence for existence multiple pathways degradation. complexity these makes it difficult obtain clear picture relative importance specific metabolic during We have developed sensitive mass spectrometry-based method simultaneously profile...
The plant hormone auxin (indole-3-acetic acid, IAA) has a crucial role in development. Its spatiotemporal distribution is controlled by combination of biosynthetic, metabolic and transport mechanisms. Four families transporters have been identified that mediate across the plasma or endoplasmic reticulum membrane. Here we report discovery functional characterization first vacuolar transporter. We demonstrate WALLS ARE THIN1 (WAT1), plant-specific protein dictates secondary cell wall thickness...
Abstract Plants are necessarily highly competitive and have finely tuned mechanisms to adjust growth development in accordance with opportunities limitations their environment. Sugars from photosynthesis form an integral part of this control process, acting as both energy source signaling molecules areas targeted for growth. The plant hormone auxin similarly functions a molecule driver developmental processes. Here, we show that not only do the two act concert but metabolism is itself...
Significance The maintenance of boundaries between neighboring groups distinct cell types is vital during development multicellular organisms, as cells with functions must be kept physically separated to guarantee correct control organ and body growth function. In the Arabidopsis root, transition zone a developmental boundary in meristem that separates dividing from differentiating cells. Here, we infer well-defined tightly controlled minimum hormone auxin acts signal establish position by...
Responses to drought, heat, and combined stress were compared in tobacco (Nicotiana tabacum L.) plants ectopically expressing the cytokinin oxidase/dehydrogenase CKX1 gene of Arabidopsis thaliana L. under control either predominantly root-expressed WRKY6 promoter or constitutive 35S promoter, wild type. WRKY6:CKX1 exhibited high CKX activity roots conditions. Under stress, was down-regulated concomitantly reduced degradation coincided with raised bioactive levels during early phase response,...
Strigolactones (SL) contribute to drought acclimatization in shoots, because SL-depleted plants are hypersensitive due stomatal hyposensitivity abscisic acid (ABA). However, under drought, SL biosynthesis is repressed roots, suggesting organ specificity their metabolism and role. Because can be transported acropetally, such a drop may also affect as systemic indication of stress. We investigated this hypothesis by analysing molecularly physiologically wild-type (WT) tomato (Solanum...
Auxin represents a key signal in plants, regulating almost every aspect of their growth and development. Major breakthroughs have been made dissecting the molecular basis auxin transport, perception, response. In contrast, how plants control metabolism homeostasis major form indole-3-acetic acid (IAA), remains unclear. this paper, we initially describe function Arabidopsis thaliana gene DIOXYGENASE FOR AUXIN OXIDATION 1 (AtDAO1). Transcriptional translational reporter lines revealed that...
Abstract Plant beneficial microbes mediate biocontrol of diseases by interfering with pathogens or via strengthening the host. Although phytohormones, including cytokinins, are known to regulate plant development and physiology as well immunity, their production microorganisms has not been considered a mechanism. Here we identify ability Pseudomonas fluorescens G20-18 efficiently control P. syringae infection in Arabidopsis , allowing maintenance tissue integrity ultimately biomass yield....
Arabidopsis growth and reproduction are stimulated by the endophytic fungus Piriformospora indica. The produces low amounts of auxins, but auxin levels expression auxin-regulated genes not altered in colonized roots. Also, mutants with reduced (ilr1-1, nit1-3, tfl2, cyp79 b2b3) respond to P. However, rescues dwarf phenotype overproducer sur1-1 converting free into conjugates, which also results downregulation auxin-induced IAA6 upregulation indica-induced LRR1 gene. relatively high...