A5017267821- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant responses to water stress
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Plant Physiology and Cultivation Studies
- Light effects on plants
- Plant tissue culture and regeneration
- Rice Cultivation and Yield Improvement
- Phytase and its Applications
- Plant Micronutrient Interactions and Effects
- Plant Growth Enhancement Techniques
- Plant responses to elevated CO2
- Plant-Microbe Interactions and Immunity
- RNA and protein synthesis mechanisms
- Lipid metabolism and biosynthesis
- Microbial Metabolites in Food Biotechnology
- Growth and nutrition in plants
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Mycotoxins in Agriculture and Food
- Coenzyme Q10 studies and effects
University of Nottingham
2015-2024
Biocenter Finland
2018
University of Freiburg
2010
Vlaams Instituut voor Biotechnologie
2005-2010
Ghent University
2005-2010
Max Planck Institute for Developmental Biology
2010
University of Maryland, College Park
2010
University of Warwick
1991-2008
University of Oxford
2005-2008
John Radcliffe Hospital
2008
Lateral root development inArabidopsis provides a model for the study of hormonal signals that regulate postembryonic organogenesis in higher plants.Lateral roots originate from pairs pericycle cells, several cell files positioned opposite xylem pole, initiate series asymmetric, transverse divisions.The auxin transport inhibitor N -1-naphthylphthalamic acid (NPA) arrests lateral by blocking first division(s).We investigated basis NPA action using cell-specific reporter to demonstrate pole...
Auxins represent an important class of plant hormone that regulate development. Plants use specialized carrier proteins to transport the auxin indole-3-acetic acid (IAA) target tissues. To date, efflux carrier-mediated polar has been assumed sole mode long distance IAA movement. Localization permease AUX1 in Arabidopsis root apex revealed a novel phloem-based pathway. AUX1, asymmetrically localized plasma membrane protophloem cells, is proposed promote acropetal, post-phloem movement apex....
In plants, the developmental mechanisms that regulate positioning of lateral organs along primary root are currently unknown. We present evidence on how initiation is controlled in a spatiotemporal manner model plant Arabidopsis thaliana. First, roots spaced main axis regular left-right alternating pattern correlates with gravity-induced waving and depends AUX1, an auxin influx carrier essential for gravitropic response. Second, we found priming pericycle cells might take place basal...
Abstract In Arabidopsis thaliana, lateral roots are formed from root pericycle cells adjacent to the xylem poles. Lateral development is regulated antagonistically by plant hormones auxin and cytokinin. While a great deal known about how promotes development, mechanism of cytokinin repression still unclear. Elevating levels was observed disrupt initiation regular pattern divisions that characterizes in Arabidopsis. To identify stage sensitive cytokinins, we targeted expression Agrobacterium...
Abstract Ethylene represents an important regulatory signal for root development. Genetic studies in Arabidopsis thaliana have demonstrated that ethylene inhibition of growth involves another hormone signal, auxin. This study investigated why auxin was required by to regulate growth. We initially observed positively controls biosynthesis the apex. subsequently ethylene-regulated is dependent on (1) transport from apex via lateral cap and (2) responses occurring multiple elongation zone...
Summary Much of humanity relies on rice ( Oryza sativa ) as a food source, but cultivation is water intensive and the crop vulnerable to drought high temperatures. Under climate change, periods reduced availability temperature are expected become more frequent, leading detrimental effects yields. We engineered high‐yielding cultivar ‘ IR 64’ produce fewer stomata by manipulating level developmental signal. overexpressed epidermal patterning factor Os EPF 1 , creating plants with...
Our aim was to generate and prove the concept of "smart" plants monitor plant phosphorus (P) status in Arabidopsis. Smart can be genetically engineered by transformation with a construct containing promoter gene up-regulated specifically P starvation an accessible tissue upstream marker such as beta-glucuronidase (GUS). First, using microarrays, we identified genes whose expression changed more than 2.5-fold shoots growing hydroponically when P, but not N or K, withheld from nutrient...
Auxin transport, which is mediated by specialized influx and efflux carriers, plays a major role in many aspects of plant growth development. AUXIN1 (AUX1) has been demonstrated to encode high-affinity auxin carrier. In Arabidopsis thaliana, AUX1 belongs small multigene family comprising four highly conserved genes (i.e., LIKE [LAX] LAX1, LAX2, LAX3). We report that all members this AUX/LAX display uptake functions. Despite the conservation their biochemical function, AUX1, LAX3 have...
Gravity profoundly influences plant growth and development. Plants respond to changes in orientation by using gravitropic responses modify their growth. Cholodny Went hypothesized over 80 years ago that plants bend response a gravity stimulus generating lateral gradient of regulator at an organ's apex, later found be auxin. Auxin regulates root targeting Aux/IAA repressor proteins for degradation. We used Aux/IAA-based reporter, domain II (DII)-VENUS, conjunction with mathematical model...
Auxin regulates several aspects of plant growth and development. is unique among hormones for exhibiting polar transport. Indole-3-acetic acid (IAA), the major form auxin in higher plants, a weak its intercellular movement facilitated by influx efflux carriers. Polarity provided asymmetric localization carriers (mainly PIN carriers). PIN-FORMED (PIN) P-GLYCOPROTEIN (PGP) family proteins are whereas AUXIN1/LIKE-AUX1 (AUX/LAX) Genetic biochemical evidence show that each member AUX/LAX...
Dark-grown dicotyledonous seedlings form a hook-like structure at the top of hypocotyl, which is controlled by hormones auxin and ethylene. Hook formation dependent on an signal gradient, whereas hook exaggeration part triple response provoked ethylene in dark-grown Arabidopsis seedlings. Several other light are also known to be involved development, but molecular mechanisms that lead initial installation gradient still poorly understood. In this study, we aimed unravel cross-talk between...
Abstract Auxin is a key regulator of plant growth and development. Within the root tip, auxin distribution plays crucial role specifying developmental zones coordinating tropic responses. Determining how organ-scale pattern regulated at cellular scale essential to understanding these processes are controlled. In this study, we developed an transport model based on actual cell geometries carrier subcellular localizations. We tested predictions using DII-VENUS sensor in conjunction with...
Auxin represents a key signal in plants, regulating almost every aspect of their growth and development. Major breakthroughs have been made dissecting the molecular basis auxin transport, perception, response. In contrast, how plants control metabolism homeostasis major form indole-3-acetic acid (IAA), remains unclear. this paper, we initially describe function Arabidopsis thaliana gene DIOXYGENASE FOR AUXIN OXIDATION 1 (AtDAO1). Transcriptional translational reporter lines revealed that...
Abstract Phosphate (P) is an essential macronutrient for plant growth. Roots employ adaptive mechanisms to forage P in soil. Root hair elongation particularly important since immobile. Here we report that auxin plays a critical role promoting root growth Arabidopsis response low external P. Mutants disrupting synthesis ( taa1 ) and transport aux1 attenuate the response. Conversely, targeting AUX1 expression lateral cap epidermal cells rescues this . Hence from apex differentiation zone...
Summary We report here the molecular characterisation of Arabidopsis MALE STERILITY1 gene, which is a critical sporophytic controlling factor for anther and pollen development. Homozygous ms1 mutants do not produce viable pollen, but are otherwise phenotypically normal. Degeneration occurs soon after microspore release from tetrads, at time tapetum also appears abnormally vacuolated. The MS1 gene expressed low levels in anthers closed buds, with expression stage release. No seen open...
Abstract We have investigated the subcellular localization, domain topology, and amino acid residues that are critical for function of presumptive Arabidopsis thaliana auxin influx carrier AUX1. Biochemical fractionation experiments confocal studies using an N-terminal yellow fluorescent protein (YFP) fusion observed AUX1 colocalized with plasma membrane (PM) markers. Because its PM we were able to take advantage steep pH gradient exists across plant cell investigate topology YFP as a...
One of the most striking features plant architecture is regular arrangement leaves and flowers around stem, known as phyllotaxis. Peaks in concentration hormone auxin, generated by polar localization PIN1 auxin efflux carrier, provide instructive signal for primordium initiation. This mechanism generates spacing between neighboring primordia, which results Studies role transport phyllotactic patterning have focused on PIN1-mediated efflux. Recent computer simulations indicate an additional...
The directional flow of the plant hormone auxin mediates multiple developmental processes, including patterning and tropisms. Apical basal plasma membrane localization AUXIN-RESISTANT1 (AUX1) PIN-FORMED1 (PIN1) transport components underpins directionality intercellular in Arabidopsis thaliana roots. Here, we examined mechanism polar trafficking AUX1. Real-time live cell analysis along with subcellular markers revealed that AUX1 resides at apical protophloem cells highly dynamic...