A5012239105- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Polysaccharides and Plant Cell Walls
- Selenium in Biological Systems
- Arsenic contamination and mitigation
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photosynthetic Processes and Mechanisms
- Plant Physiology and Cultivation Studies
- Aluminum toxicity and tolerance in plants and animals
- Allelopathy and phytotoxic interactions
- Edible Oils Quality and Analysis
- Lipid metabolism and biosynthesis
- Plant responses to water stress
- Glycosylation and Glycoproteins Research
- Enzyme-mediated dye degradation
- Mycorrhizal Fungi and Plant Interactions
- Trace Elements in Health
- Environmental Toxicology and Ecotoxicology
- Legume Nitrogen Fixing Symbiosis
- Garlic and Onion Studies
- Birth, Development, and Health
- Horticultural and Viticultural Research
- Essential Oils and Antimicrobial Activity
Universidad de Extremadura
2013-2024
Centre for Plant Biotechnology and Genomics
2012-2014
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2012-2014
Centro de Biología Molecular Severo Ochoa
2012
Universidad Politécnica de Madrid
2012
Ghent University
2012
Vlaams Instituut voor Biotechnologie
2012
University of Freiburg
2010
University of Nottingham
2010
Lateral root development inArabidopsis provides a model for the study of hormonal signals that regulate postembryonic organogenesis in higher plants.Lateral roots originate from pairs pericycle cells, several cell files positioned opposite xylem pole, initiate series asymmetric, transverse divisions.The auxin transport inhibitor N -1-naphthylphthalamic acid (NPA) arrests lateral by blocking first division(s).We investigated basis NPA action using cell-specific reporter to demonstrate pole...
In plants, the developmental mechanisms that regulate positioning of lateral organs along primary root are currently unknown. We present evidence on how initiation is controlled in a spatiotemporal manner model plant Arabidopsis thaliana. First, roots spaced main axis regular left-right alternating pattern correlates with gravity-induced waving and depends AUX1, an auxin influx carrier essential for gravitropic response. Second, we found priming pericycle cells might take place basal...
Abstract In Arabidopsis thaliana, lateral roots are formed from root pericycle cells adjacent to the xylem poles. Lateral development is regulated antagonistically by plant hormones auxin and cytokinin. While a great deal known about how promotes development, mechanism of cytokinin repression still unclear. Elevating levels was observed disrupt initiation regular pattern divisions that characterizes in Arabidopsis. To identify stage sensitive cytokinins, we targeted expression Agrobacterium...
Arabidopsis root architecture is regulated by shoot-derived signals such as nitrate and auxin. We report that mutations in the putative auxin influx carrier AUX1 modify a result of disruption hormone transport between indole-3-acetic acid (IAA) source sink tissues. Gas chromatography–selected reaction monitoring–mass spectrometry measurements revealed aux1 mutant exhibited altered IAA distribution young leaf tissues, major organs, respectively, developing seedling. Expression studies using...
Auxin transport, which is mediated by specialized influx and efflux carriers, plays a major role in many aspects of plant growth development. AUXIN1 (AUX1) has been demonstrated to encode high-affinity auxin carrier. In Arabidopsis thaliana, AUX1 belongs small multigene family comprising four highly conserved genes (i.e., LIKE [LAX] LAX1, LAX2, LAX3). We report that all members this AUX/LAX display uptake functions. Despite the conservation their biochemical function, AUX1, LAX3 have...
Abstract SHORT-ROOT (SHR) is a well-characterized regulator of radial patterning and indeterminacy the Arabidopsis (Arabidopsis thaliana) primary root. However, its role during elaboration root system architecture remains unclear. We report that indeterminate wild-type was transformed into determinate in shr mutant when growing soil or agar. The growth behavior results from apical meristem failing to initiate cell division following germination. inability reactivate mitotic activity...
Roots are important to plants for a wide variety of processes, including nutrient and water uptake, anchoring mechanical support, storage functions, as the major interface between plant various biotic abiotic factors in soil environment. Therefore, understanding development architecture roots holds potential manipulation root traits improve productivity sustainability agricultural systems better understand manage natural ecosystems. While lateral is traceable process along primary different...
Overall root architecture is the combined result of primary and lateral growth influenced by both intrinsic genetic programs external signals. One main questions for biologists how plants control number primordia their emergence through root. We recently identified S-phase kinase-associated protein2 (SKP2B) as a new early marker development. Here, we took advantage its specific expression pattern in Arabidopsis (Arabidopsis thaliana) cell-sorting transcriptomic approach to generate...
Abstract The endogenous circadian clock enables organisms to adapt their growth and development environmental changes. Here we describe how the is employed coordinate responses key signal auxin during lateral root (LR) emergence. In model plant, Arabidopsis thaliana , LRs originate from a group of stem cells deep within root, necessitating that new organs emerge through overlying tissues. We report rephased LR development. Metabolite transcript profiling revealed controls levels...
In Arabidopsis , lateral roots originate from pericycle cells deep within the primary root. New root primordia (LRP) have to emerge through several overlaying tissues. Here, we report that auxin produced in new LRP is transported towards outer tissues where it triggers cell separation by inducing both influx carrier LAX3 and cell‐wall enzymes. expressed just two files LRP. To understand how this striking pattern of expression regulated, developed a mathematical model captures network...
Lateral root primordia (LRP) originate from pericycle stem cells located deep within parental tissues. LRP emerge through overlying tissues by inducing auxin-dependent cell separation and hydraulic changes in adjacent cells. The auxin-inducible auxin influx carrier LAX3 plays a key role concentrating this signal LRP. Delimiting expression to two files new is crucial ensure that auxin-regulated occurs solely along their shared walls. Multiscale modeling has predicted highly focused pattern of...
Abstract In plants, lateral roots originate from pericycle founder cells that are specified at regular intervals along the main root. Here, we show Arabidopsis (Arabidopsis thaliana) SKP2B (for S-Phase Kinase-Associated Protein2B), an F-box protein, negatively regulates cell cycle and root formation as it represses meristematic divisions. According to its function, is expressed in cells, primordia apical meristem. We identified a novel motif promoter required for specific expression...
The alterations induced by the toxicity of antimony (Sb) in roots and leaves sunflower plants were determined. grown hydroponically with different concentrations Sb, a heavy metal which reduces biomass production growth. There was preferential accumulation Sb tissues roots, being much lower. other mineral elements also altered, especially that Fe Zn. Chlorophyll content declined, as did photosynthetic efficiency, but carotenoid remained unaltered. total phenolics, flavonoids, phenylpropanoid...
Roots of intact olive seedlings, axenically cultured, were alternatively placed in contact with Rhizophagus irregularis (mycorrhizal) or Verticillim dahliae (pathogenic) fungi. MeJA treatments also included. In vivo redox activities the apoplast roots (anion superoxide generation, dismutase and peroxidase activities) measured. All our results showed that apoplastic seedling compatible mycorrhizal fungus clearly attenuated comparison pathogenic treated MeJA, even at early stages treatment...
Summary Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA important root responses. Mutations FIP1 , a component machinery, affects development, cell division elongation, different abiotic stresses. Salt treatment increases amount poly(A) site usage within coding region 5′ untranslated regions (5′‐UTRs), lack activity reduces these...
This research studies the effects that Sb toxicity (0.0, 0.5, and 1.0 mM) has on growth, reactive oxygen nitrogen species, antioxidant systems in tomato plants. is accumulated preferentially roots, with little capacity for its translocation to leaves where concentration much lower. The growth of seedlings reduced, alteration content other nutrients. There a decrease Fe, Mg, Mn, while Cu Zn increase. contents chlorophyll b decrease, as does photosynthetic efficiency. On contrary carotenoids...
Abstract A key impediment to studying water-related mechanisms in plants is the inability non-invasively image water fluxes cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modelling, can achieve this goal - monitoring hydrodynamics within living root tissues cell- sub-second-scale resolutions. imaging of water-transporting xylem vessels Arabidopsis thaliana mutant roots reveals faster transport endodermal diffusion...