A5084765698- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Virus Research Studies
- Heat shock proteins research
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- RNA Research and Splicing
- Light effects on plants
- Plant biochemistry and biosynthesis
- CRISPR and Genetic Engineering
- Plant Reproductive Biology
- RNA modifications and cancer
- Plant nutrient uptake and metabolism
- Microtubule and mitosis dynamics
- Insect and Pesticide Research
- Polyamine Metabolism and Applications
- Plant-Microbe Interactions and Immunity
- Seed Germination and Physiology
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Transgenic Plants and Applications
- Animal Virus Infections Studies
- Plant Genetic and Mutation Studies
- Agronomic Practices and Intercropping Systems
Centre for Plant Biotechnology and Genomics
2015-2024
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2016-2024
Universidad Politécnica de Madrid
2016-2024
Centro de Investigaciones Biológicas Margarita Salas
2012-2019
Imperial College London
2012
John Innes Centre
2004-2008
Universidad Autónoma de Madrid
1998-2007
Consejo Superior de Investigaciones Científicas
1998-2007
Centro de Biología Molecular Severo Ochoa
1998-2004
In plants, the expression of 14-3-3 genes reacts to various adverse environmental conditions, including cold, high salt, and drought. Although these results suggest that proteins have potential regulate plant responses abiotic stresses, their role in such remains poorly understood. Previously, we showed RARE COLD INDUCIBLE 1A (RCI1A) gene encodes psi isoform. Here, present genetic molecular evidence implicating RCI1A response low temperature. Our demonstrate functions as a negative regulator...
Heat stress is one of the most prominent and deleterious environmental threats affecting plant growth development. Upon high temperatures, plants launch specialized gene expression programs that promote protection survival. These involve global specific changes at transcriptional translational levels. However, coordination these processes their role in establishment heat response not fully elucidated. We have carried out a genome-wide analysis to monitor translation efficiency individual...
In eukaryotic cells, the function of DNA replication licensing components (Cdc6 and Cdt1, among others) is crucial for cell proliferation genome stability. However, little known about their role in whole organisms whether control interfaces with differentiation developmental programs. Here, we study Arabidopsis thaliana CDT1, its regulation, consequences overriding control. The availability AtCDT1 strictly regulated at two levels: (1) transcription level, by E2F growth-arresting signals, (2)...
Studies on the CDC6 protein, which is crucial to control of DNA replication in yeast and animal cells, are lacking plants. We have isolated an Arabidopsis cDNA encoding AtCDC6 protein studied its possible connection occurrence developmentally regulated endoreplication cycles. The gene expressed maximally early S-phase, promoter contains E2F consensus site that mediates binding a plant E2F/DP complex. Transgenic plants carrying promoter–β-glucuronidase fusion revealed it active proliferating...
Abstract In yeast and animals, SM-like (LSM) proteins typically exist as heptameric complexes are involved in different aspects of RNA metabolism. Eight LSM proteins, LSM1 to 8, highly conserved form two distinct heteroheptameric complexes, LSM1-7 LSM2-8,that function mRNA decay splicing, respectively. A search the Arabidopsis thaliana genome identifies 11 genes encoding related eight LSMs, putative LSM1, LSM3, LSM6 being duplicated. Here, we report molecular functional characterization gene...
Abstract In many plant species, an exposure to a sublethal temperature triggers adaptative response called acclimation. This involves extensive molecular reprogramming that allows the further survive otherwise lethal increase of temperature. A related is also launched under abrupt and heat stress that, in this case, unable successfully promote thermotolerance therefore ends up death. Although these programmes are expected have common players, overlapping degree specific regulators each...
AN1 is a regulatory gene that promotes anthocyanin biosynthesis in potato tubers and encodes R2R3 MYB transcription factor. However, no clear evidence implicates production leaves, where these pigments might enhance environmental stress tolerance. In our study we found displays intraspecific sequence variability both coding/non-coding regions the promoter, its expression associated with high content leaves of commercial potatoes. Expression analysis provided leaf pigmentation to StJAF13 acts...
The control of precursor-messenger RNA (pre-mRNA) splicing is emerging as an important layer regulation in plant responses to endogenous and external cues. In eukaryotes, pre-mRNA governed by the activity a large ribonucleoprotein machinery, spliceosome, whose protein core composed Sm ring related Sm-like 2-8 complex. Recently, Arabidopsis (Arabidopsis thaliana) complex has been characterized. However, role proteins remains largely unknown. Here, we present functional characterization E1...
For years, the study of gene expression regulation plants in response to stress conditions has been focused mainly on analysis transcriptional changes. However, knowledge translational is very scarce these organisms, despite plants, as rest eukaryotes, proven play a pivotal role different stresses. Regulation protein synthesis under abiotic was thought be conserved process, since, general, both translation factors and process are basically similar eukaryotes. this conservation not so clear...
Plants have developed versatile strategies to deal with the great variety of challenging conditions they are exposed to. Among them, regulation translation is a common target finely modulate gene expression both under biotic and abiotic stress situations. Upon environmental challenges, regulated reduce consumption energy selectively synthesize proteins involved in proper establishment tolerance response. In case viral infections, situation more complex, as viruses evolved unconventional...
HSP70-HSP90 organizing protein (HOP) is a well-studied family of cytosolic cochaperones. However, the possible role HOP during endoplasmic reticulum (ER) stress response and identity its interactors within ER were not previously addressed in any eukaryote. We have demonstrated that Arabidopsis HOP3, whose function was studied before, interacts vivo with HSP90 HSP70, and, unexpectedly, binding immunoglobulin (BiP), HSP70 ER-resident protein. Although BiP lacks domain described other...
HSP70-HSP90 organizing protein (HOP) is a family of cytosolic cochaperones whose molecular role in thermotolerance quite unknown eukaryotes and unexplored plants. In this article, we describe that the three members AtHOP display different induction pattern under heat, being HOP3 highly regulated during challenge attenuation period. Despite most heat-regulated member, analysis hop1 hop2 hop3 triple mutant demonstrates HOP proteins act redundantly to promote long-term acquired Arabidopsis....
Summary Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA important root responses. Mutations FIP1 , a component machinery, affects development, cell division elongation, different abiotic stresses. Salt treatment increases amount poly(A) site usage within coding region 5′ untranslated regions (5′‐UTRs), lack activity reduces these...
The highly conserved Cdc6 protein is required for initiation of eukaryotic DNA replication and, in yeast and Xenopus, the coupling to mitosis. Herein, we show that human rapidly destroyed by a p53-independent, proteasome-, ubiquitin-dependent pathway during early stages programmed cell death induced DNA-damaging drug adozelesin, or separate caspase-dependent cells undergoing apoptosis through an extrinsic tumor necrosis factor-α cycloheximide. proteasome-dependent adozelesin budding...
Living organisms have the capacity to respond environmental stimuli, including warm conditions. Upon sensing mild temperature, plants launch a transcriptional response that promotes morphological changes, globally known as thermomorphogenesis. This is orchestrated by different hormonal networks and activity of transcription factors, heat shock factor A1 (HSFA1) family. Members this family interact with protein 70 (HSP70) 90 (HSP90); however, effect binding on regulation HSFA1 or role...