A5023333157- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Potato Plant Research
- Plant-Microbe Interactions and Immunity
- Phytochemicals and Antioxidant Activities
- Plant responses to water stress
- Plant biochemistry and biosynthesis
- Plant Pathogens and Resistance
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- RNA Research and Splicing
- Plant Virus Research Studies
- Polysaccharides and Plant Cell Walls
- Adipose Tissue and Metabolism
- Plant Reproductive Biology
- Mechanisms of cancer metastasis
- Plant Water Relations and Carbon Dynamics
- Magnetic and Electromagnetic Effects
- ATP Synthase and ATPases Research
- RNA modifications and cancer
- Plant Disease Resistance and Genetics
- Plant responses to elevated CO2
National Research Council
2013-2024
Institute of Biosciences and Bioresources
2014-2024
University of Turin
2022
Purdue University West Lafayette
2014-2016
Instituto di Biofisica
2016
National Academies of Sciences, Engineering, and Medicine
2013
King Abdullah University of Science and Technology
2010
University of California, Riverside
2007-2010
Université de Strasbourg
2009
University of Naples Federico II
2007
Sucrose nonfermenting 1 (SNF1)-related protein kinase 2s (SnRK2s) are central components of abscisic acid (ABA) signaling pathways. The snrk2.2/2.3/2.6 triple-mutant plants nearly completely insensitive to ABA, suggesting that most the molecular actions ABA triggered by SnRK2s-mediated phosphorylation substrate proteins. Only a few proteins SnRK2s known. To identify additional and provide insight into we used quantitative phosphoproteomics compare global changes in phosphopeptides WT triple...
The salt overly sensitive (SOS) pathway is critical for plant stress tolerance and has a key role in regulating ion transport under stress.To further investigate factors regulated by the SOS pathway, we expressed an N-terminal fusion of improved tandem affinity purification tag to SOS2 (NTAP-SOS2) sos2-2 mutant plants.Expression NTAP-SOS2 rescued defect plants, indicating that protein was functional vivo.Tandem NTAP-SOS2-containing complexes subsequent liquid chromatography-tandem mass...
AbstractSOS2, a class 3 sucrose-nonfermenting 1-related kinase, has emerged as an important mediator of salt stress response and signaling through its interactions with proteins involved in membrane transport regulation responses. We have identified additional SOS2-interacting that suggest connection between SOS2 reactive oxygen signaling. was found to interact the H2O2 protein nucleoside diphosphate kinase 2 (NDPK2) inhibit autophosphorylation activity. A sos2-2 ndpk2 double mutant more...
Abstract Anthocyanins are major pigments in colored grape (Vitis vinifera) berries, and most of them monomethoxylated or dimethoxylated. We report here the functional characterization an anthocyanin O-methyltransferase (AOMT) from grapevine. The expression pattern two cultivars with different methylation profiles (Syrah Nebbiolo) showed a peak at start ripening (véraison), when concentrations all methylated anthocyanins begin to increase. purified recombinant AOMT protein was active on both...
Drought is a major constraint for plant growth and crop productivity that receiving an increased attention due to global climate changes. Chloroplasts act as environmental sensors, however, only partial information available on stress-induced mechanisms within plastids. Here, we investigated the chloroplast response severe drought treatment subsequent recovery cycle in tomato through physiological, metabolite proteomic analyses. Under stress conditions, plants showed stunted growth, elevated...
Phenylpropanoids are major secondary metabolites in eggplant (Solanum melongena) fruits. Chlorogenic acid (CGA) accounts for 70-90% of total phenolics flesh tissues, while anthocyanins mainly present the fruit skin. As a contribution to understanding peculiar accumulation these health-promoting eggplant, we report on metabolite abundance, regulation CGA and anthocyanin biosynthesis, characterization candidate biosynthetic genes S. melongena. Higher contents CGA, Delphinidin 3-rutinoside,...
Tomato is a major crop in the Mediterranean basin, where cultivation open field often vulnerable to drought. In order adapt and survive naturally occurring cycles of drought stress recovery, plants employ coordinated array physiological, biochemical, molecular responses. Transcriptomic studies on tomato responses subsequent recovery are few number. As search for novel traits improve genetic tolerance increases, better understanding these required. To address this need we designed study which...
AN1 is a regulatory gene that promotes anthocyanin biosynthesis in potato tubers and encodes R2R3 MYB transcription factor. However, no clear evidence implicates production leaves, where these pigments might enhance environmental stress tolerance. In our study we found displays intraspecific sequence variability both coding/non-coding regions the promoter, its expression associated with high content leaves of commercial potatoes. Expression analysis provided leaf pigmentation to StJAF13 acts...
The phytohormone abscisic acid (ABA) regulates plant growth, development and responses to biotic abiotic stresses. core ABA signaling pathway consists of three major components: receptor (PYR1/PYLs), type 2C Protein Phosphatase (PP2C) SNF1-related protein kinase 2 (SnRK2). Nevertheless, the complexity remains be explored. To uncover new components signal transduction pathways, we performed a yeast two-hybrid screen for SnRK2-interacting proteins. We found that Type One 1 (TOPP1) its...
Summary DEAD ‐box RNA helicases are involved in many aspects of metabolism and diverse biological processes plants. Arabidopsis thaliana mutants two helicases, STRESS RESPONSE SUPPRESSOR 1 ( STRS 1) 2 were previously shown to exhibit tolerance abiotic stresses up‐regulated stress‐responsive gene expression. Here, we show that A rabidopsis ‐overexpressing lines displayed a less tolerant phenotype reduced expression stress‐induced genes confirming the s as attenuators stress responses. GFP –...
Salt and drought stress severely reduce plant growth crop productivity worldwide. The identification of genes underlying response tolerance is the subject intense research in biology. Through microarray analyses, we previously identified potato (Solanum tuberosum) StRGGA, coding for an Arginine Glycine (RGG) box-containing RNA-binding protein, whose expression was specifically induced cell cultures gradually exposed to osmotic stress. Here, show that Arabidopsis (Arabidopsis thaliana)...
Tomato is a high value crop and the primary model for fleshy fruit development ripening. Breeding priorities include increased quality, shelf life tolerance to stresses. To contribute towards this goal, we re-sequenced genomes of Corbarino (COR) Lucariello (LUC) landraces, which both possess traits plant adaptation water deficit, prolonged shelf-life good quality. Through newly developed pipeline Reconstructor, generated genome sequences COR LUC using datasets 65.8 M 56.4 30-150 bp...
Wild potato species are useful sources of allelic diversity and loci lacking in the cultivated potato. In these species, presence anthocyanins leaves has been associated with a greater tolerance to cold stress. However, molecular mechanisms that allow potatoes withstand exposure remain unclear. Here, we show expression AN2, MYB transcription factor, is induced by low temperatures wild, cold-tolerant Solanum commersonii, not susceptible tuberosum varieties. We found AN2 paralog anthocyanin...
The Mediterranean basin countries are considered secondary centres of tomato diversification. However, information on phenotypic and allelic variation local materials is still limited. Here we report the evaluation largest traditional collection, which includes 1499 accessions from Southern Europe. Analyses 70 traits revealed a broad range variability with different distributions among countries, culinary end use within each country being main driver Furthermore, eight types (phenoclusters)...
Despite intense research towards the understanding of abiotic stress adaptation in tomato, physiological adjustments and transcriptome modulation induced by combined salt low nitrate (low N) conditions remain largely unknown. Here, three traditional tomato genotypes were grown under long-term single stresses throughout a complete growth cycle. Physiological, molecular, measurements showed extensive morphophysiological modifications compared to control, conditions, resulting highest penalty...
Summary Modulation of growth in response to environmental cues is a fundamental aspect plant adaptation abiotic stresses. TIP 41 (TAP42 INTERACTING PROTEIN OF kDa) the Arabidopsis thaliana orthologue proteins isolated mammals and yeast that participate Target‐of‐Rapamycin ( TOR ) pathway, which modifies cell nutrient status conditions. Here, we characterized function Arabidopsis. Expression analyses showed constitutively expressed vascular tissues, induced following long‐term exposure NaCl,...
Abscisic acid (ABA) plays an important role in various aspects of plant growth and development, including adaptation to stresses, fruit development ripening. In seeds, ABA participates through its core signaling components dormancy instauration, longevity determination, inhibition germination unfavorable environmental conditions such as high soil salinity. Here, we show that seed pepper was delayed but only marginally reduced by or NaCl with respect control treatments. Through a similarity...
RNA splicing is a fundamental mechanism contributing to the definition of cellular protein population in any given environmental condition. DNA-DAMAGE REPAIR/TOLERATION PROTEIN111 (DRT111)/SPLICING FACTOR FOR PHYTOCHROME SIGNALING factor previously shown interact with phytochrome B and characterized for its role pre-mRNAs involved photomorphogenesis. Here, we show that DRT111 interacts Arabidopsis (Arabidopsis thaliana) Splicing Factor1, 3′ site recognition. Double- triple-mutant analysis...