A5003393368- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- RNA Research and Splicing
- RNA modifications and cancer
- Plant biochemistry and biosynthesis
- Lipid metabolism and biosynthesis
- Chromosomal and Genetic Variations
- Estrogen and related hormone effects
- Light effects on plants
- Plant Micronutrient Interactions and Effects
- Heat shock proteins research
- Plant Genetic and Mutation Studies
- Legume Nitrogen Fixing Symbiosis
- Algal biology and biofuel production
- RNA and protein synthesis mechanisms
- Radiopharmaceutical Chemistry and Applications
- Polysaccharides and Plant Cell Walls
- Cancer Research and Treatments
- Drug Transport and Resistance Mechanisms
- Enzyme Catalysis and Immobilization
- Plant-Microbe Interactions and Immunity
University of Maryland, College Park
2012-2023
Anhui Agricultural University
2022-2023
Jinan University
2021
Jiangsu University of Science and Technology
2017-2019
Hunan Agricultural University
2017-2018
Zhejiang University
2016
State Key Laboratory of Plant Physiology and Biochemistry
2016
First Affiliated Hospital of Sichuan Medical University
2014
Southwest Medical University
2014
University of California, Riverside
2004-2010
Summary The abiotic stresses of drought, salinity and freezing are linked by the fact that they all decrease availability water to plant cells. This decreased is quantified as a in potential. Plants resist low potential related modifying uptake loss avoid potential, accumulating solutes properties cell walls dehydration induced using protective proteins mechanisms tolerate reduced content preventing or repairing damage. Salt stress also alters ion homeostasis, under many conditions this may...
Drought, high salinity and freezing impose osmotic stress on plants. Plants respond to the in part by modulating gene expression, which eventually leads restoration of cellular homeostasis, detoxification toxins recovery growth. The signal transduction pathways mediating these adaptations can be dissected combining forward reverse genetic approaches with molecular, biochemical physiological studies. Arabidopsis is a useful model system for this purpose its relatives including halophyte...
Abstract Nuclear factor Y (NF-Y) is a ubiquitous transcription composed of three distinct subunits (NF-YA, NF-YB, and NF-YC). We found that the Arabidopsis thaliana NFYA5 transcript strongly induced by drought stress in an abscisic acid (ABA)-dependent manner. Promoter:β-glucuronidase analyses showed was highly expressed vascular tissues guard cells part induction transcriptional. contains target site for miR169, which targets mRNAs cleavage or translational repression. miR169 downregulated...
Abstract Salt cress (Thellungiella halophila) is a small winter annual crucifer with short life cycle. It has genome (about 2 × Arabidopsis) high sequence identity (average 92%) Arabidopsis, and can be genetically transformed by the simple floral dip procedure. capable of copious seed production. an extremophile native to harsh environments reproduce after exposure extreme salinity (500 mm NaCl) or cold −15°C. typical halophyte that accumulates NaCl at controlled rates also dramatic levels...
microRNAs (miRNAs) play important roles in plant growth and development. Previous studies have shown that down-regulation of miR398 response to oxidative stress permits up-regulation one its target genes, CSD2 (copper/zinc superoxide dismutase), thereby helps plants cope with stress. We report here heat rapidly induces reduces transcripts genes CSD1, CCS (a gene encoding a copper chaperone for both CSD1 CSD2). Transgenic expressing miR398-resistant forms under the control their native...
An Arabidopsis thaliana mutant, cryophyte, was isolated and found to have an enhanced cold stress-induction of the master regulator tolerance, C-repeat binding factor 2 (CBF2), its downstream target genes. The mutant is more tolerant chilling freezing stresses but sensitive heat stress. Under warm not growth temperatures, has a reduced stature flowers earlier. long day conditions, flowering insensitive vernalization. also hypersensitive phytohormone abscisic acid. mutation in DEAD box RNA...
Cold acclimation involves precise signaling and regulation of the transcriptome. The plasma membrane may be primary cold‐stress sensor, FRY1/HOS2 inositol polyphosphate 1‐phosphatase regulates cytosolic inositol‐1,4,5‐triphosphate levels, which in turn control Ca 2+ signatures cold acclimation. Cold‐induced reactive oxygen species activate a mitogen‐activated protein kinase cascade (AtMEKK1‐AtMKK2‐AtMPK4/6) that tolerance to freezing other abiotic stresses. induces expression C‐repeat...
Histone modification in chromatin is one of the key control points gene regulation eukaryotic cells. Protein complexes composed histone acetyltransferase or deacetylase, WD40 repeat protein, and many other components have been implicated this process. Here, we report identification functional characterization HOS15, a WD40-repeat protein crucial for repression genes associated with abiotic stress tolerance through deacetylation Arabidopsis . HOS15 shares high sequence similarity human...
Summary Apple ( Malus × domestica ) trees are vulnerable to freezing temperatures. However, there has been only limited success in developing cold‐hardy cultivars. This lack of progress is due at least partly understanding the molecular mechanisms tolerance apple. In this study, we evaluated potential roles for two R2R3 MYB transcription factors TF s), 88 and paralogous FLP 124), cold stress apple Arabidopsis . We found that 124 positively regulate cold‐responsive gene expression both...
Abstract The production of anthocyanin is regulated by light and corresponding photoreceptors. In this study, we found that exposure to blue overexpression CRY1a are associated with increased accumulation in tomato ( Solanum lycopersicum L.). These responses the result changes mRNA protein levels SlHY5 , which a transcription factor. vitro vivo experiments using electrophoretic mobility shift assay ChIP‐qPCR assays revealed could directly recognize bind G‐box ACGT‐containing element...
To investigate essential components mediating stress signaling in plants, we initiated a large-scale response screen using Arabidopsis plants carrying the firefly luciferase reporter gene under control of stress-responsive RD29A promoter. Here report identification and characterization mutant, hos9-1 (for high expression o s motically responsive genes), which construct was hyperactivated by low temperature, but not abscisic acid or salinity stress. The mutants grow more slowly, flower later,...
The adverse effects of high salt on plants include Na(+) toxicity and hyperosmotic oxidative stresses. plasma membrane-localized Na(+)/H(+) antiporter SOS1 functions in the extrusion toxic from cells is essential for plant tolerance. We report here that, under or stress, interacts through its predicted cytoplasmic tail with RCD1, a regulator oxidative-stress responses. Without stress treatment, RCD1 localized nucleus. Under found not only nucleus but also cytoplasm. Like rcd1 mutants, sos1...
Post-translational modifications of histones play crucial roles in the genetic and epigenetic regulation gene expression from chromatin. Studies mammals yeast have found conserved at some residues as well non-conserved other sites. Although plants been excellent systems to study regulation, histone are known critical roles, modification sites patterns poorly defined. In present we used mass spectrometry combination with high performance liquid chromatography (HPLC) separation phospho-peptide...
The Arabidopsis DNA glycosylase/lyase ROS1 participates in active demethylation by a base-excision pathway [1Gong Z. Morales-Ruiz T. Ariza R.R. Roldan-Arjona David L. Zhu J.K. ROS1, repressor of transcriptional gene silencing Arabidopsis, encodes glycosylase/lyase.Cell. 2002; 111: 803-814Abstract Full Text PDF PubMed Scopus (476) Google Scholar, 2Agius F. Kapoor A. Role the demethylation.Proc. Natl. Acad. Sci. USA. 2006; 103: 11796-11801Crossref (208) Scholar]. has been shown to be required...
To identify the genetic loci that control salt tolerance in higher plants, a large-scale screen was conducted with bialaphos marker-based T-DNA insertional collection of Arabidopsis ecotype C24 mutants. One line, osm1 (for osmotic stress-sensitive mutant), exhibited increased sensitivity to both ionic (NaCl) and nonionic (mannitol) stress root-bending assay. The mutant displayed more branched root pattern or without hypersensitive inhibition by Na(+), K(+), Li(+) but not Cs(+). Plants also...
AbstractSOS2, a class 3 sucrose-nonfermenting 1-related kinase, has emerged as an important mediator of salt stress response and signaling through its interactions with proteins involved in membrane transport regulation responses. We have identified additional SOS2-interacting that suggest connection between SOS2 reactive oxygen signaling. was found to interact the H2O2 protein nucleoside diphosphate kinase 2 (NDPK2) inhibit autophosphorylation activity. A sos2-2 ndpk2 double mutant more...
Abstract In plants, many gene transcripts are very unstable, which is important for the tight control of their temporal and spatial expression patterns. To identify cellular factors controlling stability unstable mRNAs in we used luciferase imaging Arabidopsis thaliana to isolate a recessive mutant, stabilized1-1 (sta1-1), with enhanced normally transcript. The sta1-1 mutation also causes stabilization some endogenous has range developmental stress response phenotypes. STA1 encodes nuclear...