A5070589231- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Genetic Mapping and Diversity in Plants and Animals
- Vector-Borne Animal Diseases
- Plant Molecular Biology Research
- Animal Virus Infections Studies
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Chromosomal and Genetic Variations
- Genetic and phenotypic traits in livestock
- Plant Stress Responses and Tolerance
- Cancer-related molecular mechanisms research
- Gut microbiota and health
- Plant Gene Expression Analysis
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Gene expression and cancer classification
- MicroRNA in disease regulation
- Plant responses to elevated CO2
- RNA Research and Splicing
- Circular RNAs in diseases
- Meat and Animal Product Quality
- Lipid metabolism and biosynthesis
- Virus-based gene therapy research
Lanzhou Veterinary Research Institute
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Lanzhou University
2022-2025
Shandong Agricultural University
2016-2025
Nanjing Agricultural University
2015-2024
Ministry of Agriculture and Rural Affairs
2022-2024
Gansu Provincial Center for Disease Control and Prevention
2024
The Synergetic Innovation Center for Advanced Materials
2020-2022
South China Agricultural University
2022
Aarhus University
2022
Completion of genome sequences for the diploid Setaria italica reveals features C4 photosynthesis that could enable improvement polyploid biofuel crop switchgrass (Panicum virgatum). The genetic basis biotechnologically relevant traits, including drought tolerance, photosynthetic efficiency and flowering control, is also highlighted. We generated a high-quality reference sequence foxtail millet (Setaria italica). ∼400-Mb assembly covers ∼80% >95% gene space. was anchored to 992-locus map...
In stark contrast to Arabidopsis, a related species, Thellungiella halophila (Thellungiella salsuginea; salt cress), displays extreme tolerance high salinity, low humidity and freezing. High nucleotide sequence identity permits the use of tools developed for Arabidopsis transcript profiling, which microarray platform with >25,000 DNA elements (70-mer oligonucleotides) was used. Microarray profiling intensity analysis, quantitative RT-PCR, metabolite profiles define genes pathways that showed...
Abstract Salt cress (Thellungiella halophila) is a small winter annual crucifer with short life cycle. It has genome (about 2 × Arabidopsis) high sequence identity (average 92%) Arabidopsis, and can be genetically transformed by the simple floral dip procedure. capable of copious seed production. an extremophile native to harsh environments reproduce after exposure extreme salinity (500 mm NaCl) or cold −15°C. typical halophyte that accumulates NaCl at controlled rates also dramatic levels...
Abstract The gut microbiota plays an important role in nutrient digestibility animals. To examine changes the pig across growth stages and its effects on digestion, population pigs at 28 days (before weaning) 60, 90 150 of age was assessed by 16S rDNA gene sequencing. apparent crude fiber (CF), neutral detergent (NDF), acid (ADF), protein (CP) ether extract (EE) also these pigs. A total 19,875 operational taxonomic units (OTUs) were identified from all samples. Both bacterial abundance...
Abstract Cassava is a major tropical food crop in the Euphorbiaceae family that has high carbohydrate production potential and adaptability to diverse environments. Here we present draft genome sequences of wild ancestor domesticated variety cassava comparative analyses with partial inbred line. We identify 1,584 1,678 gene models specific varieties, respectively, discover heterozygosity millions single-nucleotide variations. Our reveal genes involved photosynthesis, starch accumulation...
Abstract The transcription regulatory network inside a eukaryotic cell is defined by the combinatorial actions of factors (TFs). However, TF binding studies in plants are too few number to produce general picture this complex network. In study, we use large-scale ChIP-seq reconstruct it maize leaf, and train machine-learning models predict co-localization. resulting covers 77% expressed genes, shows scale-free topology functional modularity like real-world sequence preferences conserved...
Abstract The Farm Animal Genotype-Tissue Expression (FarmGTEx) project has been established to develop a public resource of genetic regulatory variants in livestock, which is essential for linking polymorphisms variation phenotypes, helping fundamental biological discovery and exploitation animal breeding human biomedicine. Here we show results from the pilot phase PigGTEx by processing 5,457 RNA-sequencing 1,602 whole-genome sequencing samples passing quality control pigs. We build pig...
Thellungiella halophila, a close relative of Arabidopsis, is model halophyte used to study plant salt tolerance. The proteomic/physiological/transcriptomic analyses leaves subjected different salinity levels, reported herein, indicate an extraordinary ability adapt large concentrations exogenous saline by compartmentalizing Na+ into cell vacuoles and accumulating proline soluble sugars as organic osmolytes. Salinity stress stimulated the accumulation starch in chloroplasts, which resulted...
Abstract Motivation: RNA-seq technology has been widely adopted as an attractive alternative to microarray-based methods study global gene expression. However, robust statistical tools analyze these complex datasets are still lacking. By grouping genes with similar expression profiles across treatments, cluster analysis provides insight into functions and networks, hence is important technique for data analysis. Results: In this manuscript, we derive clustering algorithms based on...
Auxin plays critical roles in many aspects of plant growth and development. Although a number auxin biosynthetic pathways have been identified, their overlapping nature has prevented clear elucidation biosynthesis. Recently, Arabidopsis (Arabidopsis thaliana) mutants with supernormal phenotypes reported. These exhibit hyperactivation genes belonging to the YUCCA family, encoding putative flavin monooxygenase enzymes that result increased endogenous levels. Here, we report discovery fertile...
In plants, the basic helix-loop-helix (bHLH) transcription factors play key roles in diverse biological processes. Genome-wide comprehensive and systematic analyses of bHLH proteins have been well conducted Arabidopsis, rice, tomato other plant species. However, only few family genes functional characterized maize.In this study, our genome-wide analysis identified 208 putative (ZmbHLH proteins) maize (Zea mays). We classified these into 18 subfamilies by comparing ZmbHLHs with Arabidopsis...
Glucocorticoid (GC) induced osteoporosis (GIO) is caused by the long-term use of GC for treatment autoimmune and inflammatory diseases. The related disruption bone marrow microcirculation increased adipogenesis contribute to GIO development. However, neither currently available anti-osteoporosis agent completely addressed adipogenesis. Salvianolic acid B (Sal B) a polyphenolic compound from Chinese herbal medicine, Salvia miltiorrhiza Bunge. aim this study was determine effects Sal on...
Abstract Broomcorn millet ( Panicum miliaceum L.) has strong tolerance to abiotic stresses, and is probably one of the oldest crops, with its earliest cultivation that dated back ca . ~10,000 years. We report here genome assembly through a combination PacBio sequencing, BioNano, Hi-C (in vivo) mapping. The 18 super scaffolds cover ~95.6% estimated (~887.8 Mb). There are 63,671 protein-coding genes annotated in this tetraploid genome. About ~86.2% syntenic foxtail have two homologous copies...
Non-coding cis-regulatory variants in animal genomes are an important driving force the evolution of transcription regulation and phenotype diversity. However, cistrome dynamics plants remain largely underexplored. Here, we compare binding GOLDEN2-LIKE (GLK) factors tomato, tobacco, Arabidopsis, maize rice. Although function GLKs is conserved, most their sites species-specific. Conserved often found near photosynthetic genes dependent on GLK for expression, but non-differentially expressed...
Abstract To fully unlock the potential of pigs as both agricultural species for animal-based protein food and biomedical models human biology disease, a comprehensive understanding molecular cellular mechanisms underlying various complex phenotypes in how findings can be translated to other species, especially humans, are urgently needed. Here, within Farm animal Genotype-Tissue Expression (FarmGTEx) project, we build PigBiobank (http://pigbiobank.farmgtex.org) systematically investigate...
A Free-Air CO(2) Enrichment (FACE) experiment compared the physiological parameters, transcript and metabolite profiles of Arabidopsis thaliana Columbia-0 (Col-0) Cape Verde Island (Cvi-0) at ambient (approximately 0.375 mg g(-1)) elevated 0.550 ([CO(2)]). Photoassimilate pool sizes were enhanced in high [CO(2)] an ecotype-specific manner. Short-term growth stimulated carbon gain irrespective down-regulation plastid functions altered expression genes involved nitrogen metabolism resembling...
Abstract Background Elucidation of the pig transcriptome is essential for interpreting functional elements genome and understanding genetic architecture complex traits such as fat deposition, metabolism growth. Results Here we used massive parallel high-throughput RNA sequencing to generate a high-resolution map porcine mRNA miRNA in liver, longissimus dorsi abdominal from two full-sib F 2 hybrid pigs with segregated phenotypes on growth, blood physiological biochemical parameters,...