A5002624697- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant responses to water stress
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Plant Water Relations and Carbon Dynamics
- Plant Physiology and Cultivation Studies
- Plant Disease Resistance and Genetics
- Plant Stress Responses and Tolerance
- Horticultural and Viticultural Research
- Potato Plant Research
- Light effects on plants
- Seed Germination and Physiology
- Phytoplasmas and Hemiptera pathogens
- Plant Gene Expression Analysis
- Transgenic Plants and Applications
- Plant-Microbe Interactions and Immunity
- Plant Taxonomy and Phylogenetics
- Insect-Plant Interactions and Control
- Phytase and its Applications
- Mycorrhizal Fungi and Plant Interactions
Cornell University
2013-2025
Purdue University West Lafayette
2014
University of North Texas
2004-2012
Shandong Agricultural University
2012
China Agricultural University
2011
University of California, San Diego
2007-2009
University of California, Berkeley
2009
Agriaquaculture Nutritional Genomic Center
2009
University of Colorado Boulder
2005
University of Illinois Urbana-Champaign
1994-1997
STRUCTURAL MATURATION OF LEAVES ... Basipetal Maturation .. . .... Timing of the Sink-Source Transition Development Minor Veins Bidirectional Transport
Mechanisms of phloem loading in the minor veins leaves are known for only a few species. We propose that there limited number strategies primary photoassimilates, sucrose and sugar alcohols. These can be predicted based on thermodynamic anatomical considerations identified by autoradiography following uptake (14)C-labeled compounds, analysis leaf solute composition concentrations, plasmodesmatal counting. Experiments 45 dicotyledonous species patterns. Over 50-fold differences concentrations...
Of all the intricate cell–cell interactions in nature, those between sieve element (SE) and its companion cell (CC) rank among most complex mysterious. Mature SEs are enucleate retain only a highly degenerate cytoplasm, yet they can remain viable functional for decades ([
Using noninvasive imaging techniques, we compared phloem unloading of the membrane-impermeant, fluorescent solute carboxyfluorescein (CF) with that potato virus X expressing gene for green protein. Although systemic transport took considerably longer to occur than did CF transport, both and occurred predominantly from class III vein network, a highly branched veinal system found between II veins. The minor veins (classes IV V) played no role in or import but were shown be functional xylem at...
Abstract C4 grasses, such as maize (Zea mays), have high photosynthetic efficiency through combined biochemical and structural adaptations. photosynthesis is established along the developmental axis of leaf blade, leading from an undifferentiated base just above ligule into highly specialized mesophyll cells (MCs) bundle sheath (BSCs) at tip. To resolve kinetics development differentiation to obtain a systems-level understanding formation, accumulation profiles proteomes isolated BSCs with...
Phloem loading, the active accumulation of photosynthate in minor veins, is thought to be motivating force for translocation. Can loading take place along an entirely symplastic route, through plasmodesmata, from mesophyll phloem? Transport small molecules plasmodesmata apparently passive, and concept phloem thus appears violate thermodynamic principles. Nonetheless, evidence such a pathway many plants has been accumulating steadily answer argument put forward. The proposed mechanism...
It is generally assumed that the primary role of phloem loading to drive long-distance transport by elevating hydrostatic pressure in sieve elements. This concept consistent with fact that, many plants, energy used increase concentrations photoassimilates leaf
Although transport of organic nutrients and regulatory molecules in the phloem underlies all aspects growth development, we still cannot say with confidence that adequately understand how it is motivated.A major problem difficult to reconcile theory pressure flow, proposed over 80 years ago by Ernst Mu ¨nch (1927), large trees.Significantly, does not scale plant size.Also, very high turgor herbs suggests has functions beyond sap conduction.A recent resurgence interest this field may lead a...
More than a quarter of the primary productivity on land, and large fraction food that humans consume, is contributed by plants fix atmospheric CO2 C4 photosynthesis. It has been estimated transferring pathway to C3 crops could boost yield 50% also increase water use efficiency reduce need for fertilizer, particularly in dry, hot environments. The high maize (Zea mays), sugarcane (Saccharum spp.) several emerging bioenergy grasses due largely photosynthesis, which enabled orderly arrangement,...
In planta RNAi against essential insect genes offers a promising route to control crop pests, but is constrained for many groups, notably phloem sap-feeding hemipterans, by poor efficacy. This study conducted on the phloem-feeding whitefly Bemisia tabaci reared tomato plants investigated causes of low efficacy and routes ameliorate problem. Experiments using transgenic lines containing ds-GFP (green fluorescent protein) revealed that full-length dsRNA phloem-mobile, ingested insects,...
Recent heterograft analyses showed that large-scale messenger RNA (mRNA) movement takes place in the phloem, but number of mobile transcripts reported varies widely. However, our knowledge mechanisms underlying mRNA remains limited. In this study, using a Nicotiana benthamiana/tomato (Solanum lycopersicum) system and transgenic approach involving potato tuberosum), we found that: (1) overall abundance leaf is not good indicator transcript mobility to root; (2) increasing expression levels...
Plant viruses encode movement proteins that are essential for infection of the host but not required viral replication or encapsidation. Squash leaf curl virus (SqLCV), a bipartite geminivirus with single-stranded DNA genome, encodes two proteins, BR1 and BL1, have been implicated in separate functions movement. To further elucidate these functions, we investigated nucleic acid binding properties cellular localization BL1. In this study, showed binds strongly to acids, higher affinity than...
The bacterial chloramphenicol acetyltransferase (CAT) gene was expressed in protoplasts of three important graminaceous plant species after introduction the by electroporation. Gene transfer occurred when high-voltage electric pulses were applied either directly or indirectly (without anode contact) to a solution containing plasmid DNA and rice, wheat, sorghum. indirect method more rapid, resulted higher protoplast viability, less subject contamination than direct-contact method. expression...
Plants load sugars from photosynthesizing leaves into the phloem of exporting veins either “apoplastically” (by using H + /sucrose symporters) or “symplastically” (through plasmodesmata). The ability to regulate photosynthesis in response light environment was compared among apoplastic loaders (pea and spinach) symplastic (pumpkin Verbascum phoeniceum ). were grown under low (LL) high (HL) transferred LL HL. Upon transfer, pea spinach up-regulated level found HL-acclimated plants, whereas...
Willow ( Salix babylonica L.) is representative of a large group plants that have extensive plasmodesmatal connections between minor vein phloem and adjoining cells. Because plasmodesmata provide diffusion pathway for small molecules, it unclear how sucrose could be loaded from the mesophyll into against concentration gradient. In studies reported here, willow leaves plasmolyzed in approximately same osmoticum as mesophyll. Sucrose concentrations cells were greater than those literature...
Photoperiod in plants is perceived by leaves and many species influences the transition to reproductive growth through long-distance signaling. CONSTANS (CO) implicated as a mediator between photoperiod perception flowering Arabidopsis. To test role of CO signaling, was expressed from promoter specific companion cells smallest veins mature leaves. This expression tissues at inception phloem translocation stream sufficient accelerate apical meristem under noninductive (short-day) conditions....
Our previous studies have shown that, while a persistent but potentially reversible suppression of the tumorous state appears to be characteristic feature vegetative phase teratoma shoot growth in crown gall disease plants, recovery from that occurs during reproductive phase. An analysis has now been made process an attempt define precise stage at which occurs. The results this diploid somatic cells teratoma-derived flower parts such as those found petals and filaments are inherently...