A5054492162- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Cardiac Imaging and Diagnostics
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Peripheral Artery Disease Management
- Eicosanoids and Hypertension Pharmacology
- Adipose Tissue and Metabolism
- Lipoproteins and Cardiovascular Health
- Plant Molecular Biology Research
- Diagnosis and Treatment of Venous Diseases
- Fatty Acid Research and Health
- Cardiac Ischemia and Reperfusion
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Coronary Interventions and Diagnostics
- Cancer, Lipids, and Metabolism
- ATP Synthase and ATPases Research
- Algal biology and biofuel production
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Pharmacology and Obesity Treatment
- Renal function and acid-base balance
- Pericarditis and Cardiac Tamponade
- Diabetic Foot Ulcer Assessment and Management
- Antiplatelet Therapy and Cardiovascular Diseases
University of Oregon
2006-2020
Protein Research Foundation
2004-2020
Osaka University
2004-2020
Cornell University
2009-2013
University of Tsukuba
1993-1999
National Cerebral and Cardiovascular Center
1992-1998
Saiseikai Kumamoto Hospital
1973
Hiroshima University
1969
Abstract Plastids contain multiple copies of the plastid chromosome, folded together with proteins and RNA into nucleoids. The degree to which components gene expression protein biogenesis machineries are nucleoid associated, factors involved in DNA organization, repair, replication, poorly understood. To provide a conceptual framework for function, we characterized proteomes highly enriched fractions proplastids mature chloroplasts isolated from maize (Zea mays) leaf base tip, respectively,...
Abstract C4 grasses, such as maize (Zea mays), have high photosynthetic efficiency through combined biochemical and structural adaptations. photosynthesis is established along the developmental axis of leaf blade, leading from an undifferentiated base just above ligule into highly specialized mesophyll cells (MCs) bundle sheath (BSCs) at tip. To resolve kinetics development differentiation to obtain a systems-level understanding formation, accumulation profiles proteomes isolated BSCs with...
Chloroplasts import thousands of nucleus-encoded preproteins synthesized in the cytosol through TOC and TIC translocons on outer inner envelope membranes, respectively. Preprotein translocation across membrane requires ATP; however, motor has remained unclear. Here, we report that a 2-MD heteromeric AAA-ATPase complex associates with functions as motor, directly interacting various translocating preproteins. This consists protein encoded by previously enigmatic chloroplast gene ycf2 five...
The clpr2-1 mutant is delayed in development due to reduction of the chloroplast ClpPR protease complex. To understand role Clp proteases plastid biogenesis and homeostasis, leaf proteomes young seedlings wild type were compared using large scale mass spectrometry-based quantification an LTQ-Orbitrap spectral counting with significance determined by G-tests. Virtually only chloroplast-localized proteins significantly affected, indicating that molecular phenotype was confined chloroplast. A...
Chloroplasts in angiosperms contain at least seven nucleus-encoded members of the DEAD box RNA helicase family. Phylogenetic analysis shows that five these plastid (RH22, -39, -47, -50, and -58) form a single clade RH3 forms with two mitochondrial RH proteins (PMH1 -2) functioning intron splicing. The function chloroplast maize (Zea mays; ZmRH3) Arabidopsis (Arabidopsis thaliana; AtRH3) was determined. ZmRH3 AtRH3 are both under strong developmental control, abundance sharply peaked...
Whereas the plastid caseinolytic peptidase (Clp) P protease system is essential for plant development, substrates and substrate selection mechanisms are unknown. Bacterial ClpS involved in N-degron delivery to ClpAP protease. Through phylogenetic analysis, we show that all angiosperms contain ClpS1 some species also ClpS1-like protein(s). In silico analysis suggests functional homolog of bacterial ClpS. We Arabidopsis thaliana interacts with ClpC1,2 chaperones. The null mutant (clps1) lacks...
Abstract Chloroplast genomes in plants and green algae contain numerous group II introns, large ribozymes that splice via the same chemical steps as spliceosome-mediated splicing nucleus. Most chloroplast introns are degenerate, requiring interaction with nucleus-encoded proteins to vivo. Genetic approaches maize (Zea mays) Chlamydomonas reinhardtii have elucidated distinct sets of assemble facilitate splicing. Little information is available, however, concerning these processes Arabidopsis...
The CRS1–YhbY domain (also called the CRM domain) is represented as a stand-alone protein in Archaea and Bacteria, family of single- multidomain proteins plants. function this unknown, but structural data presence several known to interact with RNA have led proposal that it binds RNA. Here we describe phylogenetic analysis domain, its incorporation into diverse plants, biochemical properties prokaryotic eukaryotic representative family. We show bacterial member family, Escherichia coli YhbY,...
The CRM domain is a recently recognized RNA binding found in three group II intron splicing factors chloroplasts, bacterial protein that associates with ribosome precursors, and family of uncharacterized proteins plants. To elucidate the functional repertoire domains, we studied CFM2 (for Family Member 2), which harbors four domains. coimmunoprecipitation assays showed maize (Zea mays) chloroplasts associated I pre-trnL-UAA introns ndhA ycf3 pre-mRNAs. T-DNA insertions Arabidopsis thaliana...
In photosynthetic eukaryotes, thousands of proteins are translated in the cytosol and imported into chloroplast through concerted action two translocons-termed TOC TIC-located outer inner membranes envelope, respectively. The degree to which molecular composition TIC complexes is conserved over phylogenetic distances has remained controversial. Here, we combine transcriptomic, biochemical, genetic tools green alga Chlamydomonas (Chlamydomonas reinhardtii) demonstrate that, despite a lack...
Abstract A chloroplast signal recognition particle (SRP) that is related to the SRP involved in secretion bacteria and eukaryotic cells used for insertion of light-harvesting chlorophyll proteins (LHCPs) into thylakoid membranes. conserved component mechanism a membrane-bound receptor, denoted FtsY bacteria. Plant genomes encode homologs are targeted (cpFtsY). To investigate vivo roles cpFtsY, we characterized maize cpFtsY mutants having Mu transposon corresponding gene (chloroplast...
Chloroplast genomes in angiosperms encode ∼20 group II introns, approximately half of which are classified as subgroup IIB. The splicing all but one the IIB introns requires a heterodimer containing peptidyl-tRNA hydrolase homolog CRS2 and two closely related proteins, CAF1 or CAF2, that harbor recently recognized RNA binding domain called CRM domain. Two CRS2/CAF-dependent require, addition, protein CFM2 is only distantly to CAF2. Here, we show CFM3, close relative CFM2, associates vivo...
The insertion of light-harvesting chlorophyll proteins (LHCPs) into the thylakoid membrane chloroplast is cpSRP-dependent, and requires stromal components cpSRP54 cpSRP43, membrane-bound SRP receptor cpFtsY integral protein Alb3. Previous studies demonstrated that Arabidopsis mutant lacking both cpSRP43 had pale yellow leaves, but was viable, whereas mutants Alb3 exhibit an albino phenotype more severe seedling lethality. We previously showed a maize yellow-green lethal. To compare in vivo...
The effect of glycemic control on the incidence restenosis after percutaneous transluminal coronary angioplasty (PTCA) in non-insulin-dependent diabetes mellitus (NIDDM) has not been well analysed.Out 1282 consecutive patients who had undergone elective and successful PTCA over 8 years, 86 known to have NIDDM 117 non-diabetic cases were analysed for following PTCA. Definition is an increase from 50% 75% diameter stenosis at same lesion within 1 year angioplasty. Those with familial...
To investigate insulin insensitivity and its reversibility, we performed an sensitivity test using the steady state plasma glucose (SSPG) method in 10 lean hypertensive subjects with normal tolerance before after treatment witz α1-blocker bunazosin, 14 age body mass index-adjusted healthy control subjects. Steady was significantly higher compared group (182 α mg/dL ν 104 7, P < .01, mean standard error of (SEM). decreased to 136 12 (P .01) a decrease blood pressure. Hypertensive had shown...
The chloroplast RNA splicing and ribosome maturation (CRM) domain is a RNA-binding found in plant-specific protein family whose characterized members play essential roles group I II introns mitochondria chloroplasts. Together, these proteins are required for of the majority approximately 20 land plants. Here, we provide evidence from Setaria viridis maize that an uncharacterized member this family, CRM Family Member1 (CFM1), promotes most had not previously been shown to require protein. A...
Ibudilast is a prostacyclin-mediated vasodilator and antiplatelet agent. The hemodynamic effects of ibudilast were evaluated in 41 patients with non-insulin-dependent diabetes mellitus by means two-dimensional Doppler ultrasonography laser blood flowmetry. Before one hour after oral administration (10 mg), or elastase (1800 U) as control, the cross-sectional area (CSA) dorsal pedis artery, its flow index (BFI), dermal microcirculatory volume (MBV) measured. In group, all parameters (CSA,...