A5007388877- Circadian rhythm and melatonin
- Hemoglobin structure and function
- Receptor Mechanisms and Signaling
- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Retinal Development and Disorders
- Magnetism in coordination complexes
- Ion channel regulation and function
- Protein Structure and Dynamics
- Metal complexes synthesis and properties
- Hemoglobinopathies and Related Disorders
- Neuroscience and Neuropharmacology Research
- MicroRNA in disease regulation
- Sperm and Testicular Function
- Nicotinic Acetylcholine Receptors Study
- Gene expression and cancer classification
- Cellular transport and secretion
- Genomics, phytochemicals, and oxidative stress
- Molecular Biology Techniques and Applications
- Metal-Organic Frameworks: Synthesis and Applications
- Protist diversity and phylogeny
- RNA Interference and Gene Delivery
- Erythrocyte Function and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
- Cholesterol and Lipid Metabolism
University of Geneva
2009-2020
SIB Swiss Institute of Bioinformatics
2013
Genomics (United Kingdom)
2005-2006
Tohoku University
2004
University of Lausanne
1996-1997
Institut Pasteur
1997
Roche (Switzerland)
1996
National Institute of Diabetes and Digestive and Kidney Diseases
1993
National Institutes of Health
1993
Inserm
1990
The mammalian circadian timing system consists of a master pacemaker in neurons the suprachiasmatic nucleus (SCN) and clocks similar molecular makeup most peripheral body cells. Peripheral oscillators are self-sustained cell autonomous, but they have to be synchronized by SCN ensure phase coherence within organism. In principle, rhythmic expression genes organs could thus driven not only local oscillators, also systemic signals. To discriminate between these mechanisms, we engineered mouse...
The three-dimensional solution structure of a complex between the DNA binding domain chicken erythroid transcription factor GATA-1 and its cognate site has been determined with multidimensional heteronuclear magnetic resonance spectroscopy. consists core which contains zinc coordinated by four cysteines carboxyl-terminal tail. is composed two irregular antiparallel β sheets an α helix, followed long loop that leads into amino-terminal part core, including similar in structure, although not...
In mammals, many aspects of behavior and physiology, in particular cellular metabolism, are coordinated by the circadian timing system. Molecular clocks thought to rely on negative feedback loops clock gene expression that engender oscillations accumulation transcriptional regulatory proteins, such as orphan receptor REV-ERBα. Circadian transcription factors then drive daily rhythms clock-controlled output genes, for example genes encoding enzymes regulators metabolism. To gain insight into...
In liver, most metabolic pathways are under circadian control, and hundreds of protein-encoding genes thus transcribed in a cyclic fashion. Here we show that rhythmic transcription extends to the locus specifying miR-122, highly abundant, hepatocyte-specific microRNA. Genetic loss-of-function gain-of-function experiments have identified orphan nuclear receptor REV-ERBalpha as major regulator mir-122 transcription. Although due its long half-life mature miR-122 accumulates at nearly constant...
The segmental ligand 2-{6-[N,N-diethylcarbamoyl]pyridin-2-yl}-1,1'-dimethyl-5,5'-methylene-2'-(5-methylpyridin-2-yl)bis[1H-benzimidazole] (L2) reacts with stoichiometric amounts of Ln(III) (Ln = La−Nd, Sm−Tb, Tm−Lu, Y) and Zn(II) in acetonitrile to yield quantitatively selectively the heterodinuclear triple-helical complexes [LnZn(L2)3]5+ under thermodynamic control. crystal structure [EuZn(L2)3](ClO4)(CF3SO3)4(CH3CN)4 (13; EuZnC111H111N25O19F12S4Cl, monoclinic, C2/c, Z 8) shows wrapping...
Sterols and sphingolipids are limited to eukaryotic cells, their interaction has been proposed favor formation of lipid microdomains. Although there is abundant biophysical evidence demonstrating in simple systems, convincing lacking show that they function together cells. Using analysis by mass spectrometry a genetic approach on mutants sterol metabolism, we cells adjust membrane composition response mutant structures preferentially changing sphingolipid composition. Systematic combination...
Abstract The cerebral cortex is organized into specialized sensory areas, whose initial territory determined by intracortical molecular determinants. Yet, cortical area size appears to be fine tuned during development respond functional adaptations. Here we demonstrate the existence of a prenatal sub-cortical mechanism that regulates areas in mice. This mediated spontaneous thalamic calcium waves propagate among sensory-modality nuclei up and provide means communication systems. Wave pattern...
Mouse sex determination provides an attractive model to study how regulatory genetic networks and signaling pathways control cell specification fate decisions. This characterizes in detail the essential role played by insulin receptor (INSR) IGF type I (IGF1R) adrenogenital development primary determination. Constitutive ablation of insulin/IGF pathway led reduced proliferation rate somatic progenitor cells both XX XY gonads prior together with downregulation hundreds genes associated...
Several million people are exposed to dioxin and dioxin-like compounds, primarily through food consumption. Skin lesions historically called "chloracne" the most specific sign of abnormal exposure classically used as a key marker in humans. We followed for 5 years man who had been toxic dioxin, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), at single oral dose million-fold more than accepted daily general population. adopted molecular medicine approach, aimed identifying appropriate therapy....
Abstract Plastid protein homeostasis is critical during chloroplast biogenesis and responses to changes in environmental conditions. Proteases molecular chaperones involved plastid quality control are encoded by the nucleus except for catalytic subunit of ClpP, an evolutionarily conserved serine protease. Unlike its Escherichia coli ortholog, this protease essential cell viability. To study function, we used a recently developed system repressible gene expression alga Chlamydomonas...
Although reverse genetics has been used to elucidate the function of numerous chloroplast proteins, characterization essential plastid genes and their role in biogenesis cell survival not yet achieved. Therefore, we developed a robust repressible gene expression system unicellular alga Chlamydomonas reinhardtii based mainly on vitamin-repressible riboswitch, this study two genes: ribosomal protein S12 (rps12), encoding protein, rpoA, α-subunit bacterial-like RNA polymerase. Repression either...
The function of the nuclear receptor Rev-erbα (Nr1d1) in brain is, apart from its role circadian clock mechanism, unknown. Therefore, we compared gene expression profiles between wild-type and knock-out (KO) animals. We identified fatty acid binding protein 7 (Fabp7, Blbp) as a direct target repression by REV-ERBα. Loss manifested memory mood related behavioral phenotypes led to overexpression Fabp7 various areas including subgranular zone (SGZ) hippocampus, where neuronal progenitor cells...
Sertoli cells (SCs) are the central, essential coordinators of spermatogenesis, without which germ cell development cannot occur. We previously showed that Dicer, an RNaseIII endonuclease required for microRNA (miRNA) biogenesis, is absolutely to mature, survive, and ultimately sustain development. Here, using isotope-coded protein labeling, a technique relative quantification by mass spectrometry, we investigated impact cell-Dicer subsequent miRNA loss on testicular proteome. found that,...
Summary The disturbed erythropoiesis in patients with refractory anaemia ring‐sideroblasts (RARS) is characterized by intramedullary apoptosis of erythroid precursors and increased iron accumulation mitochondria. To gain insight into these pathophysiological mechanisms we compared the gene expression profile (GEP) from RARS to GEP normal precursors. Three hundred sixty four probe sets were up‐, 253 downregulated cells. Interestingly, Growth Differentiation factor 15 (GDF15), a cytokine TGFβ...
The transcriptional response to EGFR activation is rapidly initiated after receptor stimulation before degradative sorting occurs.
Autophagy is a eukaryotic catabolic process also participating in cell-autonomous defence. Infected host cells generate double-membrane autophagosomes that mature autolysosomes to engulf, kill and digest cytoplasmic pathogens. However, several bacteria subvert autophagy benefit from its machinery functions. Monitoring infection stages by genetics, pharmacology microscopy, we demonstrate the ESX-1 secretion system of Mycobacterium marinum, close relative M. tuberculosis, upregulates...
The regulatory pathways required to maintain eukaryotic lipid homeostasis are largely unknown. We developed a systematic approach uncover new players in the regulation of homeostasis. Through an unbiased mass spectrometry-based lipidomic screening, we quantified hundreds species, including glycerophospholipids, sphingolipids, and sterols, from collection 129 mutants protein kinase phosphatase genes Saccharomyces cerevisiae. Our successfully identified known kinases involved uncovered ones....
Abstract Pioneering studies within the last few years have allowed in vitro expansion of tissue‐specific adult stem cells from a variety endoderm‐derived organs, including stomach, small intestine, and colon. Expansion these requires activation receptor Lgr5 by its ligand R‐spondin 1 is likely facilitated fact that healthy adults organs are highly proliferative. In many other such as liver, proliferating normally not abundant adulthood. However, upon injury, liver has strong regenerative...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelf-Assembly of Heteronuclear Supramolecular Helical Complexes with Segmental LigandsClaude Piguet, Gerard Hopfgartner, Bernard Bocquet, Olivier Schaad, and Alan F. WilliamsCite this: J. Am. Chem. Soc. 1994, 116, 20, 9092–9102Publication Date (Print):October 1, 1994Publication History Published online1 May 2002Published inissue 1 October 1994https://pubs.acs.org/doi/10.1021/ja00099a027https://doi.org/10.1021/ja00099a027research-articleACS...
Failure of the testes to descend into scrotum (cryptorchidism) is one most common birth defects in humans. In utero exposure estrogens, such as 17β-estradiol (E2) or synthetic estrogen diethylstilbestrol (DES), down-regulates insulin-like 3 (Insl3) expression embryonic Leydig cells, which turn results cryptorchidism mice. To identify molecular mechanism whereby xenoestrogens block Insl3 gene transcription, we performed a microarray analysis wild-type receptor (ER) α-mutant exposed...