A5054276215- Metabolomics and Mass Spectrometry Studies
- Lipid metabolism and biosynthesis
- Sphingolipid Metabolism and Signaling
- Lipid Membrane Structure and Behavior
- Trypanosoma species research and implications
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Gut microbiota and health
- Ultrasound and Hyperthermia Applications
- ATP Synthase and ATPases Research
- Liver Disease Diagnosis and Treatment
- Immune cells in cancer
- Tuberculosis Research and Epidemiology
- Cellular transport and secretion
- Cancer-related molecular mechanisms research
- Bacteriophages and microbial interactions
- Photosynthetic Processes and Mechanisms
- Advanced Proteomics Techniques and Applications
- Research on Leishmaniasis Studies
- Advanced biosensing and bioanalysis techniques
- Insect symbiosis and bacterial influences
- Peroxisome Proliferator-Activated Receptors
- Plant biochemistry and biosynthesis
Nanyang Technological University
2015-2024
Harbin Medical University
2012-2024
Xinqiao Hospital
2022-2024
Army Medical University
2020-2024
University of Jinan
2024
First Affiliated Hospital of Harbin Medical University
2012-2022
Liaoning University of Traditional Chinese Medicine
2020
Army Medical College
2020
Swiss Tropical and Public Health Institute
2012-2015
University of Basel
2012-2015
Recognition of lipids by proteins is important for their targeting and activation in many signaling pathways, but the mechanisms that regulate such interactions are largely unknown. Here, we found binding to ubiquitous lipid phosphatidic acid (PA) depended on intracellular pH protonation state its phosphate headgroup. In yeast, a rapid decrease response glucose starvation regulated PA transcription factor, Opi1, coordinately repressed phospholipid metabolic genes. This enabled coupling...
Abstract microbeMASST, a taxonomically informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging curated database of >60,000 monocultures, users can known and unknown MS/MS spectra link them to their respective producers via fragmentation patterns. Identification microbe-derived metabolites relative without priori knowledge will vastly enhance the understanding microorganisms’ role ecology human health.
Sterols and sphingolipids are limited to eukaryotic cells, their interaction has been proposed favor formation of lipid microdomains. Although there is abundant biophysical evidence demonstrating in simple systems, convincing lacking show that they function together cells. Using analysis by mass spectrometry a genetic approach on mutants sterol metabolism, we cells adjust membrane composition response mutant structures preferentially changing sphingolipid composition. Systematic combination...
Recent rapid growth of lipidomics is mainly attributed to technological advances in mass spectrometry. Development soft ionization techniques, combination with computational tools, has spurred subsequent development various methods for lipid analysis. However, none these existing approaches can cover major cellular lipids a single run. Here we demonstrate that method liquid chromatography coupled spectrometry (LCMS) be used simultaneous profiling including glycerophospholipids (PLs),...
The mitochondrial inner membrane contains different translocator systems for the import of presequence-carrying proteins and carrier proteins. assembly maintenance protein 41 (Tam41/mitochondrial matrix 37) was identified as a new member by its role in maintaining integrity activity presequence translocase (TIM23 complex). Here we demonstrate that imported translocase, TIM22 complex, is even more strongly affected lack Tam41. Moreover, respiratory chain supercomplexes potential are impaired...
Influenza virus acquires a host-derived lipid envelope during budding, yet convergent view on the role of host metabolism infection is lacking. Using mass spectrometry-based lipidomics approach, we provide systems-scale perspective membrane dynamics infected human lung epithelial cells and purified influenza virions. We reveal enrichment minor peroxisome-derived ether-linked phosphatidylcholines relative to bulk ester-linked in virions as unique pathogenicity-dependent signature for not...
Altered lipid metabolism in macrophages is associated with various important inflammatory conditions. Although an target for therapeutic intervention, the metabolic requirement involved accumulation during pro-inflammatory activation of remains incompletely characterized. We show here that macrophage IFNγ results increased aerobic glycolysis, iNOS-dependent inhibition respiration, and triacylglycerol. Surprisingly, metabolite tracing 13C-labeled glucose revealed contributed to glycerol...
The rapid increase in lipidomic studies has led to a collaborative effort within the community establish standards and criteria for producing, documenting, disseminating data. Creating dynamic easy-to-use checklist that condenses key information about experiments into common terminology will enhance field's consistency, comparability, repeatability. Here, we describe structure rationale of established Lipidomics Minimal Reporting Checklist transparency lipidomics research.
Abstract Lipids are rapidly moving to centre stage in many fields of biological sciences. Lipidomics, the systems‐level scale analysis lipids and their interacting factors, is thus an emerging field which holds great promise for drug biomarker discovery. Here we present a mass spectrometry‐based approach profiling polar lipids, particular phospholipids sphingolipids, Saccharomyces cerevisiae . The first step includes semi‐quantitative surveys untargeted fashion, particularly powerful...
Kainate is a glutamate analog that has been widely used in pharmacological studies of neuronal injury related to ischemic conditions and epilepsy. While altered lipid metabolism implicated kainate action, no study yet investigated the associated changes metabolites on systems scale. Here we describe mass spectrometry-based approach for profiling mixtures nontargeted fashion. Combined with tandem spectrometry, this method aims identify lipids are between two conditions, kainate-treated...
Autophagy is a lysosomal-mediated degradation process that promotes cell survival during nutrient-limiting conditions. However, excessive autophagy results in death. In Drosophila, regulated nutritionally, hormonally and developmentally several tissues, including the fat body, nutrient-storage organ. Here, we use proteomics approach to identify components of starvation-induced autophagic responses Drosophila body. Using cICATTM labeling mass spectrometry, differences protein expression...
Newly assembled dengue viruses (DENV) undergo maturation to become infectious particles. The process involves major rearrangement of virus surface premembrane (prM) and envelope (E) proteins. prM-E complexes on immature are first as trimeric spikes in the neutral pH environment endoplasmic reticulum. When is transported low exosomes, these rearrange into dimeric structures, which lie parallel lipid envelope. proteins involved driving this unknown. Previous cryoelectron microscopy studies...
Abstract The immune response to mycobacteria is characterized by granuloma formation, which features multinucleated giant cells as a unique macrophage type. We previously found that result from Toll-like receptor-induced DNA damage and cell autonomous cycle modifications. However, the progenitor identity remained unclear. Here, we show cell-forming potential particular trait of monocyte progenitors. Common progenitors potently produce cytokines in their immune-active molecules. In addition,...
Synthetic genetic array analyses identify powerful interactions between a thermosensitive allele (sec14-1 ts ) of the structural gene for major yeast phosphatidylinositol transfer protein (SEC14) and deletion (tlg2Δ) Tlg2 target membrane-soluble N-ethylmaleimide-sensitive factor attachment receptor. The data further demonstrate Sec14 is required proper trans-Golgi network (TGN)/endosomal dynamics in yeast. Paradoxically, combinatorial depletion activities elicits trafficking defects from...
Phosphatidic acid (PA) is a key regulated intermediate and precursor for de novo biosynthesis of all glycerophospholipids. PA can be synthesized through the acylation lysophosphatidic (LPA) by 1-acyl-3-phosphate acyltransferase (also called acyltransferase, LPAAT). Recent findings have substantiated essential roles acyltransferases in various biological functions.We used flow-injection-based lipidomic approach with approximately 200 multiple reaction monitoring (MRM) transitions to...