Lisa Panzenboeck
A5067460377- Metabolomics and Mass Spectrometry Studies
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Sphingolipid Metabolism and Signaling
- Lipid Membrane Structure and Behavior
- Tea Polyphenols and Effects
- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
- Machine Learning in Materials Science
- Metabolism, Diabetes, and Cancer
- Proteoglycans and glycosaminoglycans research
- Advanced Proteomics Techniques and Applications
- Photoreceptor and optogenetics research
- Isotope Analysis in Ecology
- Lysosomal Storage Disorders Research
- Computational Drug Discovery Methods
- Spectroscopy and Quantum Chemical Studies
- Analytical Chemistry and Chromatography
- Microbial Metabolic Engineering and Bioproduction
- Traditional Chinese Medicine Analysis
- Lipid metabolism and biosynthesis
University of Vienna
2020-2024
Abstract microbeMASST, a taxonomically informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging curated database of >60,000 monocultures, users can known and unknown MS/MS spectra link them to their respective producers via fragmentation patterns. Identification microbe-derived metabolites relative without priori knowledge will vastly enhance the understanding microorganisms’ role ecology human health.
State-of-the-art quantitative metabolomics relies on isotope dilution using internal standards (IS) derived from fully
Abstract MicrobeMASST, a taxonomically-informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging curated database of >60,000 monocultures, users can known and unknown MS/MS spectra link them to their respective producers via fragmentation patterns. Identification microbial-derived metabolites relative producers, without priori knowledge, will vastly enhance the understanding microorganisms’ role ecology...
Gangliosides are an indispensable glycolipid class concentrated on cell surfaces with a critical role in stem differentiation. Nonetheless, owing to the lack of suitable methods for scalable analysis covering full scope ganglioside molecular diversity, their mechanistic properties signaling and differentiation remain undiscovered large extent. This work introduces sensitive comprehensive assay based liquid chromatography, high-resolution mass spectrometry, multistage fragmentation....
Abstract MicrobeMASST, a taxonomically-informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging curated database of >60,000 monocultures, users can known and unknown MS/MS spectra link them to their respective producers via fragmentation patterns. Identification microbial-derived metabolites relative producers, without priori knowledge, will vastly enhance the understanding microorganisms' role ecology...
We introduce TRAM, a triple acquisition strategy on high-speed, time-of-flight mass spectrometry for merging non-targeted and targeted metabolomics into one run. TRAM stands “quasi-simultaneous” of (1) full scan MS1, (2) top 30 data-dependent MS2 (DDA), (3) scheduled multiple reaction monitoring (MRM) within measurement cycles ~ 1 second. combines the selectivity sensitivity state-of-the-art MRM-based methods with scope analysis enabled by high-resolution spectrometry. In this work, we...
Covalent or non-covalent heterogeneous multimerization of molecules associated with extracts from biological samples analyzed via LC-MS are quite difficult to recognize/annotate and therefore the prevalence remains largely unknown. In this study, we utilized 13C labeled unlabeled Pichia pastoris recognize multimers. More specifically, between 0.8% 1.5% biologically-derived features detected in our experiments were confirmed be heteromers, about half which could successfully annotate...
Glycosyl inositol phospho ceramides (GIPCs) are the major sphingolipids on earth, as they account for a considerable fraction of total lipids in plants and fungi, which turn represent large portion biomass earth. Despite their obvious importance, GIPC analysis remains challenging due to lack commercial standards automated annotation software. In this work, we introduce novel glycolipidomics workflow based reversed-phase ultra-high pressure liquid chromatography coupled high-resolution mass...
We introduce TRAM, a triple acquisition strategy on high-speed quadrupole time-of-flight mass spectrometer for merging non-targeted and targeted metabolomics into one run. TRAM stands "quasi-simultaneous" of (1) full scan MS1, (2) top 30 data-dependent MS2 (DDA), (3) scheduled multiple reaction monitoring (MRM) within measurement cycles ∼1 s. combines the selectivity sensitivity state-of-the-art MRM-based methods with scope analysis enabled by high-resolution spectrometry.
Abstract Covalent or non-covalent heterogeneous multimerization of molecules associated with extracts from biological samples analyzed via LC-MS is quite difficult to recognize/annotate and therefore the prevalence remains largely unknown. In this study, we utilized 13C labeled unlabeled Pichia pastoris recognize multimers. More specifically, between 0.8% 1.5% biologically-derived features detected in our experiments were confirmed be heteromers, about half which could successfully annotate...
Abstract Gangliosides are an indispensable glycolipid class concentrated on cell surfaces with a critical role in stem differentiation. Nonetheless, owing to the lack of suitable methods for scalable analysis covering full scope ganglioside molecular diversity, their mechanistic properties signaling and differentiation remain undiscovered large extent. This work introduces sensitive comprehensive assay based liquid chromatography, high-resolution mass spectrometry, multistage fragmentation....