Felina Hildebrand
A5063512720- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Gut microbiota and health
- Analytical Chemistry and Chromatography
- Genomics and Phylogenetic Studies
- Advanced Proteomics Techniques and Applications
- Diet and metabolism studies
University of Vienna
2020-2025
Faculty (United Kingdom)
2021
Abstract microbeMASST, a taxonomically informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging curated database of >60,000 monocultures, users can known and unknown MS/MS spectra link them to their respective producers via fragmentation patterns. Identification microbe-derived metabolites relative without priori knowledge will vastly enhance the understanding microorganisms’ role ecology human health.
In this study, we developed a customized high-resolution mass spectrometry metabolomics workflow integrating the dual sugar test employing lactulose and mannitol as probes for intestinal permeability assessment with untargeted screening of small molecules. Urine samples were collected from patients major depression healthy controls part clinical study at psychiatric department. Using injection/dual chromatography setup, quantified by hydrophilic interaction liquid (HILIC) in targeted assay,...
A fully automated novel workflow for lipidomics based on flow injection, followed by reversed phase liquid chromatography–high-resolution mass spectrometry in combination with LILY as internal standard, enabling accurate quantification<italic>via</italic>RP-LC.
Abstract MicrobeMASST, a taxonomically-informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging curated database of >60,000 monocultures, users can known and unknown MS/MS spectra link them to their respective producers via fragmentation patterns. Identification microbial-derived metabolites relative producers, without priori knowledge, will vastly enhance the understanding microorganisms’ role ecology...
Ion mobility-mass spectrometry (IM-MS) offers benefits for lipidomics by obtaining IM-derived collision cross sections (CCS), a conditional physicochemical parameter of an ion which can enhance lipid identification. While drift tube (DT) IM-MS retains direct link to the primary experimental method derive CCS values, other IM technologies rely solely on external calibration, posing challenges due dissimilar chemical properties between lipids and calibrants. To address this, we introduce...
Ion mobility–mass spectrometry (IM-MS) offers benefits for lipidomics by obtaining IM-derived collision cross sections (CCS), a conditional property of an ion that can enhance lipid identification. While drift tube (DT) IM-MS retains direct link to the primary experimental method derive CCS values, other IM technologies rely solely on external calibration, posing challenges due dissimilar chemical properties between lipids and calibrants. To address this, we introduce MobiLipid, novel tool...
Abstract MicrobeMASST, a taxonomically-informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging curated database of >60,000 monocultures, users can known and unknown MS/MS spectra link them to their respective producers via fragmentation patterns. Identification microbial-derived metabolites relative producers, without priori knowledge, will vastly enhance the understanding microorganisms' role ecology...
ABSTRACT We propose a fully automated novel workflow for lipidomics based on flow injection-followed by liquid chromatography high resolution mass spectrometry (FI/LC-HRMS). The combined in-depth characterization of the lipidome achieved via reversed phase LC-HRMS with absolute quantification as obtained number lipid species-specific- and/or retention time (RT) matched/class-specific calibrants. 13 C labelled yeast (LILY) provided cost efficient, large panel internal standards covering...