A5001006603- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- High Altitude and Hypoxia
- Reproductive System and Pregnancy
- Atherosclerosis and Cardiovascular Diseases
- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- vaccines and immunoinformatics approaches
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Animal Virus Infections Studies
- Diet and metabolism studies
- Inflammasome and immune disorders
- Tryptophan and brain disorders
- Gut microbiota and health
Medical University of Vienna
2022-2025
To investigate long COVID-19 syndrome (LCS) pathophysiology, we performed an exploratory study with blood plasma derived from three groups: 1) healthy vaccinated individuals without SARS-CoV-2 exposure; 2) asymptomatic recovered patients at least months after infection and; 3) symptomatic 3 chronic fatigue or similar symptoms, here designated as (LCS). Multiplex cytokine profiling indicated slightly elevated pro-inflammatory levels in contrast to LCS. Plasma proteomics demonstrated low of...
In this study, we developed a customized high-resolution mass spectrometry metabolomics workflow integrating the dual sugar test employing lactulose and mannitol as probes for intestinal permeability assessment with untargeted screening of small molecules. Urine samples were collected from patients major depression healthy controls part clinical study at psychiatric department. Using injection/dual chromatography setup, quantified by hydrophilic interaction liquid (HILIC) in targeted assay,...
First-generation vaccines against SARS-CoV-2 do not provide adequate immune protection. Therefore, we engineered a divalent gene construct combining the receptor-binding domain (RBD) of spike protein and immunodominant region viral nucleocapsid. This fusion was produced in either E. coli or recombinant baculovirus system. Subsequently, mixed with adjuvant administered to mice prime-booster mode. Mice (72%) an IgG response both proteins (titer: 10−4–10−5) 14 days after first booster...
T follicular helper (Tfh) cells are essential for the development of germinal center B and high-affinity antibody-producing in humans mice. Here, we identify guanine nucleotide exchange factor (GEF) Rin-like (Rinl) as a negative regulator Tfh generation. Loss Rinl leads to an increase aging, upon vivo immunization acute LCMV Armstrong infection mice, human CD4+ cell vitro cultures. Mechanistically, adoptive transfer experiments using WT Rinl-KO naïve unraveled cell-intrinsic GEF-dependent...
Abstract First-generation vaccines against SARS-CoV-2 have been administered to more than 60% of the population in developed countries. However, monovalent currently available Europe do not confer adequate and durable immune protection. To satisfy need for a novel vaccine, we engineered divalent gene construct consisting receptor binding domain (RBD, 300-685 aa) spike protein immunodominant region nucleocapsid (100-300 aa). This fusion was cloned into pET-30a plasmid expressed either...
FOXP3 + regulatory T cells (Treg cells) are key for immune homeostasis. Here, we reveal that nuclear receptor corepressor 1 (NCOR1) controls naïve and effector Treg cell states. Upon NCOR1 deletion in cells, frequencies were elevated mice vitro-generated human cells. NCOR1-deficient failed to protect from severe weight loss intestinal inflammation associated with CD4 transfer colitis, indicating impaired suppressive function. the transcriptional integrity of since gene signatures already...
Abstract Despite the increasing prevalence of patients with Long Covid Syndrome (LCS), to date pathophysiology disease is still unclear, and therefore diagnosis therapy are a complex effort without any standardization. To address these issues, we performed broad exploratory screening study applying state-of-the-art post-genomic profiling methods blood plasma derived from three groups: 1) healthy individuals vaccinated against SARS-CoV-2 exposure full virus, 2) asymptomatic fully recovered at...
Despite the increasing prevalence of patients with Long Covid Syndrome (LCS), to date pathophysiology disease is still unclear, and therefore diagnosis therapy are a complex effort without any standardization. To address these issues, we performed broad exploratory screening study applying state-of-the-art post-genomic profiling methods blood plasma derived from three groups: 1) healthy individuals vaccinated against SARS-CoV-2 exposure full virus, 2) asymptomatic fully recovered at least...
Abstract FOXP3 + regulatory T cells (Treg cells) are key for immune homeostasis. Here, we reveal that nuclear receptor corepressor 1 (NCOR1) controls naïve and effector Treg cell states. Upon NCOR1 deletion in cells, frequencies were elevated mice vitro -generated human cells. NCOR1-deficient failed to protect from severe weight loss intestinal inflammation associated with CD4 transfer colitis, indicating impaired suppressive function. transcriptional integrity of since gene signatures...
Abstract T follicular helper (Tfh) cells are essential for the development of germinal center B and high-affinity antibody producing B-cells in human mice. Here, we identify guanine nucleotide exchange factor (GEF) Rin-like (Rinl) as a negative regulator Tfh generation. Loss Rinl leads to an increase aging, upon vivo immunization acute LCMV Armstrong infection mice, CD4 + cell vitro cultures. Further, adoptive transfer experiments using WT Rinl-KO naïve unraveled cell-intrinsic functions...