A5035889786- Advanced Proteomics Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- vaccines and immunoinformatics approaches
- Cancer, Lipids, and Metabolism
- Melanoma and MAPK Pathways
- Immune cells in cancer
- Glycosylation and Glycoproteins Research
- Computational Drug Discovery Methods
- Mass Spectrometry Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Peptidase Inhibition and Analysis
- Neuroblastoma Research and Treatments
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Nuclear Structure and Function
- Click Chemistry and Applications
- Skin and Cellular Biology Research
- S100 Proteins and Annexins
- Endoplasmic Reticulum Stress and Disease
- Protein Degradation and Inhibitors
- Proteoglycans and glycosaminoglycans research
- RNA modifications and cancer
University of Vienna
2016-2025
Medical University of Vienna
2013-2025
Creative Commons
2022
Robert Bosch (Germany)
2021
Czech Academy of Sciences, Institute of Analytical Chemistry
2018-2020
Vienna Biocenter
2016-2019
Life University
2016-2019
Austrian Economics Center
2017
Comprehensive Cancer Center Vienna
2009-2016
Christian Doppler Laboratory for Thermoelectricity
2009-2014
Members of the caspase family cysteine proteases play central roles in coordinating stereotypical events that occur during apoptosis. Because major executioner caspases, caspase-3 and caspase-7, exhibit almost indistinguishable activity toward certain synthetic peptide substrates, this has led to widespread view these occupy functionally redundant within cell death machinery. However, distinct phenotypes mice deficient either as well both, is at odds with view. These could be related...
WNT2 acts as a pro-angiogenic factor in placental vascularization and increases angiogenesis liver sinusoidal endothelial cells (ECs) other ECs. Increased expression is detectable many carcinomas participates tumor progression. In human colorectal cancer (CRC), selectively elevated cancer-associated fibroblasts (CAFs), leading to increased invasion metastasis. However, if there role for colon cancer, was not addressed so far. We demonstrate that enhances EC migration/invasion, while it...
Improving coverage, robustness and sensitivity is crucial for routine phosphoproteomics analysis by single-shot liquid chromatography tandem mass spectrometry (LC-MS/MS) from minimal peptide inputs. Here, we systematically optimized key experimental parameters automated on-beads sample preparation with focus on low input samples. Assessing the number of identified phosphopeptides, enrichment efficiency, site localization scores relative multiply-phosphorylated peptides pinpointed critical...
Uremia impairs the atheroprotective properties of HDL, but mechanisms underlying why this occurs are unknown. Here, we observed that HDL isolated from healthy individuals inhibited production inflammatory cytokines by peripheral monocytes stimulated with a Toll-like receptor 2 agonist. In contrast, majority patients ESRD did not show anti-inflammatory property; many samples even promoted cytokines. To investigate difference, used shotgun proteomics to identify 49 HDL-associated proteins in...
Abstract Organometallic metal(arene) anticancer agents require ligand exchange for their activity and this is generally believed to confer low selectivity potential cellular targets. However, using an integrated proteomics‐based target‐response profiling approach as a potent hypothesis‐generating procedure, we found unexpected target of ruthenium(arene) pyridinecarbothioamide (plecstatin) plectin, scaffold protein cytolinker, which was validated in plectin knock‐out model vitro. Plectin...
Cell surface metalloproteases coordinate signaling during development, tissue homeostasis, and disease. TACE (TNF-α-converting enzyme), is responsible for cleavage ("shedding") of membrane-tethered molecules, including the cytokine TNF, activating ligands EGFR. The trafficking within secretory pathway requires its binding to iRhom2, which mediates exit from endoplasmic reticulum. An important, but mechanistically unclear, feature biology ability be stimulated rapidly on cell by numerous...
The mevalonate pathway provides metabolites for post-translational modifications such as farnesylation, which are critical the activity of RAS downstream signaling. Subsequently occurring regulatory processes can induce an aberrant stimulation DNA methyltransferase (DNMT1) well changes in histone deacetylases (HDACs) and microRNAs many cancer cell lines. Inhibitors increasingly recognized anticancer drugs. Extensive evidence indicates intense cross-talk between signaling pathways, affect...
The remarkable feature of Schwann cells (SCs) to transform into a repair phenotype turned the spotlight on this powerful cell type. SCs provide regenerative environment for axonal re-growth after peripheral nerve injury (PNI) and play vital role in differentiation neuroblastic tumors benign subtype neuroblastoma, tumor originating from neural crest-derived neuroblasts. Hence, understanding their mode-of-action is utmost interest new approaches medicine, but also neuroblastoma therapy....
Abstract Adult Schwann cells (SCs) possess an inherent plastic potential. This plasticity allows SCs to acquire repair-specific functions essential for peripheral nerve regeneration. Here, we investigate whether stromal in benign-behaving neuroblastic tumors adopt a similar cellular state. We profile ganglioneuromas and neuroblastomas, rich poor SC stroma, respectively, nerves after injury, repair SCs. Indeed, share the expression of repair-associated genes. Neuroblastoma cells, derived from...
Metabolic biomonitoring in humans is typically based on the sampling of blood, plasma or urine. Although established clinical routine, these procedures are often associated with a variety compliance issues, which impeding time-course studies. Here, we show that metabolic profiling minute amounts sweat sampled from fingertips addresses this challenge. Sweat non-invasive, robust and can be accomplished repeatedly by untrained personnel. The matrix represents rich source for phenotyping. We...
To investigate long COVID-19 syndrome (LCS) pathophysiology, we performed an exploratory study with blood plasma derived from three groups: 1) healthy vaccinated individuals without SARS-CoV-2 exposure; 2) asymptomatic recovered patients at least months after infection and; 3) symptomatic 3 chronic fatigue or similar symptoms, here designated as (LCS). Multiplex cytokine profiling indicated slightly elevated pro-inflammatory levels in contrast to LCS. Plasma proteomics demonstrated low of...
Metastasis is the major cause of cancer-related deaths. Neuroblastoma (NB), a childhood tumor has been molecularly defined at primary cancer site, however, bone marrow (BM) as metastatic niche NB poorly characterized. Here we perform single-cell transcriptomic and epigenomic profiling BM aspirates from 11 subjects spanning three subtypes compare these to five age-matched metastasis-free BM, followed by in-depth single cell analyses tissue diversity cell-cell interactions, well functional...
The fate of cytosolic proteins was studied during Fas-induced cell death Jurkat T-lymphocytes by proteome analysis. Among 1000 spots resolved in two-dimensional gels, comparison control versus apoptotic cells revealed that the signal intensity 19 decreased or even disappeared, whereas 38 novel emerged. These were further analyzed with respect to de novo protein synthesis, phosphorylation status, and intracellular localization metabolic labeling analysis subcellular fractions combination...
Summary Atherosclerotic plaque rupture with subsequent mural thrombus formation is considered the main event compromising epicardial flow in acute myocardial infarction (AMI). However, precise mechanisms underlying coronary occlusion are unknown. We compared proteomic profiles of systemic plasma and derived from site patients AMI by two-dimensional gel electrophoresis ELISA. identified a local activation complement system, selective accumulation activator Creactive protein (CRP) downstream...
Hepatocytes are known to express a large number of characteristic proteins. Transformed and cultured hepatocytes only partially maintain functional cell differentiation characteristics, which can be assessed by proteome profiling. Here, we applied 2D-PAGE analysis in addition shotgun proteomics assess the state primary human (PHH), HepG2 Hep3B cells. Out total 1995 proteins identified cytoplasm these cells, filtered 107 for hepatocytes. A 104 those were hepatocytes, 20 HepG2, 6 Hep3B....
Endothelial cells represent major effectors in inflammation and angiogenesis, processes that drive a multitude of pathological states such as atherosclerosis cancer. Both angiogenesis are interconnected with each other the sense many pro-inflammatory proteins possess proangiogenic properties vice versa. To elucidate this interplay further, we present comparative proteome study inflammatory angiogenic activated endothelial cells. HUVEC were stimulated interleukin 1-β VEGF, respectively....
Polyphenol oxidase (PPO) is a type-3 copper enzyme catalyzing the oxidation of phenolic compounds to their quinone derivates, which are further converted melanin, ubiquitous pigment in living organisms. In this study plant originated tyrosinase was isolated from walnut leaves (Juglans regia) and biochemically characterized. It possible isolate purify by means an aqueous two-phase extraction method followed chromatographic purification identification. Interestingly, showed rather high...
The apical inflammatory cytokine TNF regulates numerous important biological processes including inflammation and cell death, drives diseases. secretion requires TACE (also called ADAM17), which cleaves from its transmembrane tether. trafficking of to the surface, stimulation proteolytic activity, depends on membrane proteins, iRhoms. To delineate how TNF/TACE/iRhom axis is regulated, we performed an immunoprecipitation/mass spectrometry screen identify iRhom-binding proteins. This...
Immune-evasion and immune checkpoints are promising new therapeutic targets for several cancer entities. In ovarian cancer, the clinical role of programmed cell death receptor ligand 1 (PD-L1) expression as mechanism to escape recognition has not been clarified yet. We analyzed PD-L1 primary peritoneal tumor tissues together with other parameters (whole transcriptomes isolated cells, local systemic cytokines metabolites) compared between recurrences. All expressed major histocompatibility...