A5052105510- Metabolomics and Mass Spectrometry Studies
- Phytochemistry and Biological Activities
- Natural product bioactivities and synthesis
- Analytical Chemistry and Chromatography
- Microbial Natural Products and Biosynthesis
- Plant biochemistry and biosynthesis
- Essential Oils and Antimicrobial Activity
- Fungal Biology and Applications
- Phytochemicals and Antioxidant Activities
- Chromatography in Natural Products
- Traditional and Medicinal Uses of Annonaceae
- Chemical synthesis and alkaloids
- Plant Pathogens and Fungal Diseases
- Traditional Chinese Medicine Analysis
- Computational Drug Discovery Methods
- Natural Compound Pharmacology Studies
- Trypanosoma species research and implications
- Bioactive Compounds and Antitumor Agents
- Phytochemistry and Bioactive Compounds
- Phytochemical compounds biological activities
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Marine Sponges and Natural Products
- Advanced Chemical Sensor Technologies
- Insect-Plant Interactions and Control
University of Geneva
2016-2025
HES-SO University of Applied Sciences and Arts Western Switzerland
2020-2025
University of Lausanne
2011-2020
University of New Caledonia
2020
Charles Humbert 8
2014-2016
Nantes Université
2016
Universidade Estadual Paulista (Unesp)
2015
Ateneo de Manila University
2012
DSM (Netherlands)
2012
ZHAW Zurich University of Applied Sciences
2012
Dereplication represents a key step for rapidly identifying known secondary metabolites in complex biological matrices. In this context, liquid-chromatography coupled to high resolution mass spectrometry (LC-HRMS) is increasingly used and, via untargeted data-dependent MS/MS experiments, massive amounts of detailed information on the chemical composition crude extracts can be generated. An efficient exploitation such data sets requires automated treatment and access dedicated fragmentation...
A new metabolite profiling approach combined with an ultrarapid sample preparation procedure was used to study the temporal and spatial dynamics of wound-induced accumulation jasmonic acid (JA) its oxygenated derivatives in Arabidopsis thaliana. In addition well known jasmonates, including hydroxyjasmonates (HOJAs), jasmonoyl-isoleucine (JA-Ile), 12-hydroxy derivative (12-HOJA-Ile), a dicarboxyjasmonate, 12-carboxyjasmonoyl-l-isoleucine (12-HOOCJA-Ile) discovered. HOJAs 12-HOOCJA-Ile were...
Leishmaniasis are diseases caused by parasites belonging to Leishmania genus. The treatment with pentavalent antimonials present high toxicity. Secondary line drugs, such as amphotericin B and miltefosine also have a narrow therapeutic index. Therefore, there is an urgent need develop new drugs treat leishmaniasis. Here, we the in vitro anti-leishmanial activity of unusual dimeric flavonoids purified from Arrabidaea brachypoda. Three compounds were tested against Leishmana sp. Compound 2 was...
Contemporary bioinformatic and chemoinformatic capabilities hold promise to reshape knowledge management, analysis interpretation of data in natural products research. Currently, reliance on a disparate set non-standardized, insular, specialized databases presents series challenges for access, both within the discipline integration interoperability between related fields. The fundamental elements exchange are referenced structure-organism pairs that establish relationships distinct molecular...
Abstract A major aim of ecology is to upscale attributes individuals understand processes at population, community and ecosystem scales. Such are typically described using functional traits, that is, standardised characteristics impact fitness via effects on survival, growth and/or reproduction. However, commonly used traits (e.g. wood density, SLA) becoming increasingly criticised for not being truly mechanistic questionable predictors ecological processes. This Special Feature reviews...
Abstract microbeMASST, a taxonomically informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging curated database of >60,000 monocultures, users can known and unknown MS/MS spectra link them to their respective producers via fragmentation patterns. Identification microbe-derived metabolites relative without priori knowledge will vastly enhance the understanding microorganisms’ role ecology human health.
African plants have long been the source of important products with nutritional and therapeutical value. Coffee originates from Ethiopia, Strophanthus species are strong arrow poisons supply cardenolides for use against cardiac insufficiency, Catharanthus roseus alkaloids well-known antileukaemic agents - just to mention a few examples. Research is continuing on vegetable material this continent in an endeavour find new compounds therapeutic interest. An outline presented here covering...
The wound response prohormone jasmonic acid (JA) accumulates rapidly in tissues both proximal and distal to injury sites plants. Using quantitative liquid chromatography-mass spectrometry after flash freezing of tissues, we found that JA accumulated within 30 s wounded Arabidopsis leaves (p = 3.5 e(-7)). augmentation wounds was strongest unwounded with direct vascular connections wherein levels increased significantly 120 wounding 0.00027). This gave conservative statistically robust...
In monocotyledonous plants, 1,4-benzoxazin-3-ones, also referred to as benzoxazinoids or hydroxamic acids, are one of the most important chemical barriers against herbivores. However, knowledge about their behavior after attack, mode action and potential detoxification by specialized insects remains limited. We chose an innovative analytical approach understand role maize 1,4-benzoxazin-3-ones in plant-insect interactions. By combining unbiased metabolomics screening simultaneous...
Summary Damage‐inducible defenses in plants are controlled part by jasmonates, fatty acid‐derived regulators that start to accumulate within 30 s of wounding a leaf. Using liquid chromatography–tandem mass spectrometry, we sought identify the 13‐lipoxygenases (13‐ LOX s) initiate wound‐induced jasmonate synthesis 190‐s timeframe Arabidopsis thaliana 19 single, double, triple and quadruple mutant combinations derived from four 13‐ genes this plant. All were found contribute wounded leaves:...
Metabolomics is playing an increasingly important role in plant science. It aims at the comprehensive analysis of metabolome which consists both primary and secondary metabolites. The goal metabolomics ultimately to identify quantify this wide array small molecules biological samples. This new science included several systems biology approaches based primarily on unbiased acquisition mass spectrometric (MS) or nuclear magnetic resonance (NMR) data from carefully selected approach provides...
Plants respond to herbivory by reprogramming their metabolism. Most research in this context has focused on locally induced compounds that function as toxins or feeding deterrents. We developed an ultra-high-pressure liquid chromatography time-of-flight mass spectrometry (UHPLC-TOF-MS)-based metabolomics approach evaluate local and systemic herbivore-induced changes maize leaves, sap, roots root exudates without any prior assumptions about function. Thirty-two differentially regulated were...
Natural products represent an inexhaustible source of novel therapeutic agents. Their complex and constrained three-dimensional structures endow these molecules with exceptional biological properties, thereby giving them a major role in drug discovery programs. However, the search for new bioactive metabolites is hampered by chemical complexity matrices which they are found. The purification single constituents from such requires significant amount work that it should be ideally performed...