A5048085886- Gut microbiota and health
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Antimicrobial Resistance in Staphylococcus
- Microbial Metabolites in Food Biotechnology
- Bacterial biofilms and quorum sensing
- Polysaccharides and Plant Cell Walls
- Streptococcal Infections and Treatments
- Aquaculture disease management and microbiota
- Enzyme Production and Characterization
- Fermentation and Sensory Analysis
- Food composition and properties
- Infective Endocarditis Diagnosis and Management
- Polysaccharides Composition and Applications
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Metabolomics and Mass Spectrometry Studies
- Bacterial Identification and Susceptibility Testing
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Infant Nutrition and Health
- Antimicrobial Peptides and Activities
- Biochemical Analysis and Sensing Techniques
- Biosensors and Analytical Detection
- bioluminescence and chemiluminescence research
Norwegian University of Life Sciences
2012-2024
University of Life Sciences in Lublin
2021
The University of Texas Health Science Center at Houston
2015-2016
The University of Texas at Austin
2014
Abstract Microbial and viral communities transform the chemistry of Earth's ecosystems, yet specific reactions catalyzed by these biological engines are hard to decode due absence a scalable, metabolically resolved, annotation software. Here, we present DRAM (Distilled Refined Annotation Metabolism), framework translate deluge microbiome-based genomic information into catalog microbial traits. To demonstrate applicability across diverse genomes, evaluated performance on defined, in silico...
Abstract β-Mannans are plant cell wall polysaccharides that commonly found in human diets. However, a mechanistic understanding into the key populations degrade this glycan is absent, especially for dominant Firmicutes phylum. Here, we show prominent butyrate-producing Firmicute Roseburia intestinalis expresses two loci conferring metabolism of β-mannans. We combine multi-“omic” analyses and detailed biochemical studies to comprehensively characterize loci-encoded proteins involved β-mannan...
Abstract microbeMASST, a taxonomically informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging curated database of >60,000 monocultures, users can known and unknown MS/MS spectra link them to their respective producers via fragmentation patterns. Identification microbe-derived metabolites relative without priori knowledge will vastly enhance the understanding microorganisms’ role ecology human health.
Beneficial modulation of the gut microbiome has high-impact implications not only in humans, but also livestock that sustain our current societal needs. In this context, we have tailored an acetylated galactoglucomannan (AcGGM) fibre to match unique enzymatic capabilities Roseburia and Faecalibacterium species, both renowned butyrate-producing commensals. Here, test accuracy AcGGM within complex endogenous pigs, wherein resolve 355 metagenome-assembled genomes together with quantitative...
The architecture of the gut bacterial ecosystem has a profound effect on physiology and well-being host. Modulation microbiota intestinal microenvironment via administration prebiotics represents valuable strategy to promote host health. This work provides insights into ability two novel wood-derived preparations, AcGGM AcAGX, influence human composition activity. These compounds were selectively fermented by commensal bacteria such as Bifidobacterium , Bacteroides - Prevotella F....
Increasing popularity of sour beer urges the development novel solutions for controlled fermentations both fast acidification and consistency in product flavor quality. One possible approach is use Saccharomyces cerevisiae co-fermentation with Lactobacillus species, which produce lactic acid as a major end-product carbohydrate catabolism. The ability lactobacilli to ferment determined by their capacity sustain brewing-related stresses, including hop iso-α acids, low pH ethanol. Here, we...
Commensal butyrate-producing bacteria belonging to the Firmicutes phylum are abundant in human gut and crucial for maintaining health. Currently, insight is lacking into how they target otherwise indigestible dietary fibers trophic interactions establish with other glycan degraders competitive environment.
Diet-microbe interactions play a crucial role in modulation of the early life microbiota and infant health. Bifidobacterium dominates breast-fed gut may persist individuals during transition from milk-based to more diversified diet. Here, we investigated adaptation longum changing nutritional environment. Genomic characterization 75 strains isolated nine either exclusively breast- or formula-fed (pre-weaning) infants their first 18 months revealed subspecies- strain-specific intra-individual...
β-mannans and xylans are important components of the plant cell wall they acetylated to be protected from degradation by glycoside hydrolases. widely present in human animal diets as fiber leguminous plants thickeners stabilizers processed foods. There many fully characterized acetylxylan esterases (AcXEs); however, enzymes deacetylating mannans less understood. Here we two carbohydrate esterases, Ri CE2 CE17, Firmicute Roseburia intestinalis , which together deacetylate complex...
EfbA is a PavA-like fibronectin adhesin of Enterococcus faecalis previously shown to be important in experimental urinary tract infection. Here, we expressed and purified the E. OG1RF confirmed that this protein binds with high affinity immobilized fibronectin, collagen I, V. We constructed an efbA deletion mutant demonstrated its virulence was significantly attenuated (P < 0.0006) versus wild type mixed inoculum rat endocarditis model. Furthermore, resulted diminished ability bind 0.0001)...
The robust physiology of Enterococcus faecalis facilitates tolerance to various stresses. We here report the transcriptional response E. V583 growth in presence 6.5% NaCl. Among early responses observed was an immediate down-regulation mscL, accompanied by up-regulation genes predicted be involved uptake extracellular potassium and glycine betaine. high NaCl concentration also induced expression chaperons cell envelope related traits, such as enterococcal polysaccharide antigen (epa) locus....
ABSTRACT A novel antimicrobial peptide designated enterocin O16 was purified from Enterococcus faecalis . Mass spectrometry showed a monoisotopic mass of 7,231 Da, and N-terminal Edman degradation identified 29-amino-acid sequence corresponding to residues 90 119 the EF_1097 protein. Bioinformatic analysis that is composed 68 most C-terminal Introduction an in-frame isogenic deletion in ef1097 gene abolished production O16. Enterocin has narrow inhibitory spectrum, as it inhibits mostly...
ABSTRACT The WxL domain recently has been identified as a novel cell wall binding found in numerous predicted proteins within multiple Gram-positive bacterial species. However, little is known about the function of containing this domain. Here, we identify and characterize 6 Enterococcus faecium which, by reverse transcription-PCR (RT-PCR) genomic analyses, are located three similarly organized operons, deemed loci A, B, C. Western blotting, electron microscopy, enzyme-linked immunosorbent...
Non-carbohydrate modifications such as acetylations are widespread in food stuffs well they play important roles diverse biological processes. These meet the gut environment and removed from their carbohydrate substrates by resident microbiota. Among most abundant O-acetylations, contributing to polysaccharides physico-chemical properties viscosity gelling ability, reducing accessibility for glycosyl hydrolases, thus hindering polysaccharide degradation. Of particular note, O-acetylations...
Cytolysin and gelatinase are prominent pathogenicity determinants associated with highly virulent Enterococcus faecalis strains. In an effort to explore the expression profiles of these virulence traits in vivo, we have employed E. variants expressing luxABCDE cassette under control either P16S, cytolysin, or promoter for infections Galleria mellonella caterpillars mice. Systemic infection G. bioluminescence-tagged MMH594 revealed temporal regulation both cytolysin promoters demonstrated...
ABSTRACT The endocarditis and biofilm-associated pili (Ebp) are important in Enterococcus faecalis pathogenesis, the pilus tip, EbpA, has been shown to play a major role biogenesis, biofilm formation, experimental infections. Based on silico analyses, we previously predicted that ATT is EbpA translational start codon, not ATG 120 bp downstream of ATT, which annotated as start. rarely used initiate protein synthesis, leading our hypothesis this codon participates regulation Ebp production. To...
Enterococcus faecalis is an opportunistic pathogen that ranks among the leading causes of biofilm-associated infections. We previously demonstrated endocarditis- and pili (Ebp) E. play a major role in biofilm formation, adherence to abiotic surfaces experimental In this study, derivatives strain OG1 were engineered further characterize functions Ebp pili. Loss resulted 36-fold decrease number closely associated cells when OG1RFΔebpABC was mixed with OG1SSpΔebpABC, compared mixing Ebp+...
Xylooligosaccharides (XOS) from woody biomass were evaluated as a substrate for secondary lactic acid bacteria (LAB) fermentation in sour beer production. XOS extracted birch (Betula pubescens) and added to promote the growth of Lactobacillus brevis BSO 464. Growth, pH, degradation, metabolic products monitored throughout fermentations, final was sensorically. utilized, compounds produced (1800 mg/L acid), pH reduced 4.1 3.6. Secondary changed sensory properties significantly, resulting...
ABSTRACT Microbial and viral communities transform the chemistry of Earth’s ecosystems, yet specific reactions catalyzed by these biological engines are hard to decode due absence a scalable, metabolically resolved, annotation software. Here, we present DRAM ( D istilled R efined A nnotation M etabolism), framework translate deluge microbiome-based genomic information into catalog microbial traits. To demonstrate applicability across diverse genomes, evaluated performance on defined, in...
ABSTRACT The interaction between bacteria and fibronectin is believed to play an important role in the pathogenicity of clinically Gram-positive cocci. In present study, we identified a gene encoding predicted f ibro n ectin-binding protein Enterococcus faeciu m ( fnm ), homologue Streptococcus pneumoniae pavA , genomes E. faecium strain TX82 all other sequenced isolates. Full-length recombinant Fnm from bound immobilized concentration-dependent manner also appeared bind collagen type V...