A5087668404- Microbial Natural Products and Biosynthesis
- Marine Sponges and Natural Products
- Essential Oils and Antimicrobial Activity
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Microbial Metabolism and Applications
- Aquatic Ecosystems and Phytoplankton Dynamics
- Computational Drug Discovery Methods
- Chemical Synthesis and Analysis
- Alkaloids: synthesis and pharmacology
- Bacterial Genetics and Biotechnology
- Silymarin and Mushroom Poisoning
- Biocrusts and Microbial Ecology
- Cholinesterase and Neurodegenerative Diseases
- Enzyme Production and Characterization
- Chemical synthesis and alkaloids
- Natural product bioactivities and synthesis
- Polysaccharides Composition and Applications
- Microbial Community Ecology and Physiology
- Food composition and properties
- Phytochemistry and Biological Activities
- Phytochemicals and Antioxidant Activities
- Bioactive Compounds and Antitumor Agents
- Medicinal Plants and Neuroprotection
- Biochemical and Structural Characterization
University of Michigan
2015-2024
Washtenaw Community College
2014-2024
Cairo University
2024
Ann Arbor Center for Independent Living
2017
Nanyang Technological University
2008-2012
Swiss Tropical and Public Health Institute
2010
University of Basel
2010
University of Waikato
2008-2010
Council of Scientific and Industrial Research
2008
National Institute of Education
2008
Lagunamides A (1) and B (2) are new cyclic depsipeptides isolated from the marine cyanobacterium Lyngbya majuscula obtained Pulau Hantu Besar, Singapore. The planar structural characterization of these molecules was achieved by extensive spectroscopic analysis, including 2D NMR experiments. In addition to Marfey's method 3JH−H coupling constant values, a modified based on Mosher's reagents analysis using LC-MS deployed for determination absolute configuration. displayed significant...
Cyanobacterial harmful algal blooms (cyanoHABs) degrade freshwater ecosystems globally. Microcystis aeruginosa often dominates cyanoHABs and produces microcystin (MC), a class of hepatotoxins that poses threats to human animal health. Microcystin toxicity is influenced by distinct structural elements across diversity related molecules encoded variant
High-throughput experimentation (HTE) is an increasingly important tool in reaction discovery. While the hardware for running HTE chemical laboratory has evolved significantly recent years, there remains a need software solutions to navigate data-rich experiments. Here we have developed phactor™, that facilitates performance and analysis of laboratory. phactor™ allows experimentalists rapidly design arrays reactions or direct-to-biology experiments 24, 96, 384, 1,536 wellplates. Users can...
Siderophores are high-affinity iron chelators produced by microorganisms and frequently contribute to the virulence of human pathogens. Targeted inhibition biosynthesis siderophores staphyloferrin B Staphylococcus aureus petrobactin Bacillus anthracis hold considerable potential as a single or combined treatment for methicillin-resistant S. (MRSA) anthrax infection, respectively. The biosynthetic pathways both involve nonribosomal peptide synthetase independent siderophore (NIS) synthetase,...
Abstract Pathogenic microorganisms often have the ability to attach a surface, building complex matrix where they colonize form biofilm. This cellular superstructure can display increased resistance antibiotics and cause serious, persistent health problems in humans. Here we describe high-throughput vitro screen identify inhibitors of Acinetobacter baumannii biofilms using library natural product extracts derived from marine microbes. Analysis Streptomyces gandocaensis results discovery...
Malbrancheamide is a dichlorinated fungal indole alkaloid isolated from both Malbranchea aurantiaca and graminicola that belongs to family of natural products containing characteristic bicyclo[2.2.2]diazaoctane core. The introduction chlorine atoms on the ring malbrancheamide differentiates it other members this contributes significantly its biological activity. In study, we characterized two flavin-dependent halogenases involved in late-stage halogenation different strains. MalA MalA′...
Abstract Even though raw mass spectrometry data is information rich, the vast majority of underutilized. The ability to interrogate these rich datasets handicapped by limited capability and flexibility existing software. We introduce Mass Spec Query Language (MassQL) that addresses issues enabling an expressive set patterns be queried directly from data. MassQL open-source query language for flexible spectrometer manufacturer-independent mining MS envision flexibility, scalability, ease use...
The rapid spread of bacterial infection caused by Staphylococcus aureus has become a problem to public health despite the presence past trials devoted controlling infection. Thus, current study aimed explore chemical composition extract endophytic fungus Aspergillus fumigatus, isolated from Albizia lucidior leaves, and investigate antimicrobial activity metabolites their probable mode actions. investigation fungal via UPLC/MS/MS led identification at least forty-two metabolites, as well...
Alzheimer's disease poses a global health concern with unmet demand requiring creative approaches to discover new medications. In this study, we investigated the chemical composition and anticholinesterase activity of Aspergillus niveus Fv-er401 isolated from Foeniculum vulgare (Apiaceae) roots. Fifty-eight metabolites were identified using UHPLC-MS/MS analysis crude extract. The fungal extract showed acetylcholinesterase (AChE) butyrylcholinesterase (BuChE) inhibitory effects IC50 53.44 ±...
Abstract Agri-food wastes, produced following industrial food processing, are mostly discarded, leading to environmental hazards and losing the nutritional medicinal values associated with their bioactive constituents. In this study, we performed a comprehensive analytical biological evaluation of selected vegetable by-products (potato, onion, garlic peels). The phytochemical analysis included UHPLC-ESI-qTOF-MS/MS in combination molecular networking determination total flavonoid phenolic...
Abstract Broccoli is commonly consumed as food and medicine. However, comprehensive metabolic profiling of two broccoli varieties, Romanesco (RB) purple (PB), in relation to their anticholinergic activity has not been fully disclosed. A total 110 compounds were tentatively identified using UPLC-Q-TOF-MS metabolomics. Distinctively different metabolomic profiles the varieties revealed by principal component analysis (PCA). Furthermore, volcano diagram analysis, it was found that PB had a...
Abstract Even though legumes are valuable medicinal plants with edible seeds that extensively consumed worldwide, there is little information available on the metabolic variations between different dietary beans and their influence as potential anti-cholinesterase agents. High-resolution liquid chromatography coupled mass spectrometry in positive negative ionization modes combined multivariate analysis were used to explore differences profiles of five commonly seeds, fava bean, black-eyed...
Indole alkaloids are a diverse class of natural products known for their wide range biological activities and complex chemical structures. Rarely observed in this indolic nitrones, such as avrainvillamide waikialoid, which possess potent bioactivities. Herein the oxa gene cluster from marine-derived fungus Penicillium oxalicum F30 is described along with characterization OxaD, flavin-dependent oxidase that generates roquefortine L, nitrone-bearing intermediate biosynthesis oxaline. Nitrone...
Onion peels are often discarded, representing an unlimited amount of food by-products; however, they a valuable source bioactive phenolics. Thus, we utilized UPLC-MS/MS to analyze the metabolomic profiles red (RO) and yellow (YO) onion peel extracts. The cytotoxic (SRB assay), anti-inflammatory (Griess antimicrobial (sensitivity test, MIC, antibiofilm, SP-SDS tests) properties were assessed in vitro. Additionally, histological analysis, immunohistochemistry, ELISA tests conducted investigate...
A case-control study of sporadic amyotrophic lateral sclerosis (ALS) in a mountainous village the French Alps discovered an association cases with history eating wild fungi (false morels) collected locally and initially identified erroneously reported as Gyromitra gigas. Specialist re-examination dried specimens ALS-associated demonstrated they were members G. esculenta group, namely venenata esculenta, species that has been to contain substantially higher concentrations gyromitrin than...
Chemical investigation of the marine cyanobacterium Lyngbya majuscula from Pulau Hantu Besar, Singapore, has led to isolation a cyclodepsipeptide, hantupeptin A (1). The planar structure 1 was assigned on basis extensive 1D and 2D NMR spectroscopic experiments. absolute configuration amino hydroxyl acid residues in molecule determined by application advanced Marfey method, chiral HPLC analysis, Mosher's method. Hantupeptin showed cytotoxicity MOLT-4 leukemia cells MCF-7 breast cancer with...
Filamentous benthic marine cyanobacteria are a prolific source of structurally unique bioactive secondary metabolites. A total 12 metabolites, belonging to the mixed polyketide–polypeptide structural class, were isolated from cyanobacterium, Lyngbya majuscula, and tested determine if they showed activity against barnacle larval settlement. The assays revealed four compounds, dolastatin 16 (1), hantupeptin C (4), majusculamide (10), isomalyngamide (12), that moderate potent anti-larval...