A5040574845- Animal testing and alternatives
- Computational Drug Discovery Methods
- Toxic Organic Pollutants Impact
- Metabolomics and Mass Spectrometry Studies
- Effects and risks of endocrine disrupting chemicals
- Insect and Pesticide Research
- Nutrition, Genetics, and Disease
- Poxvirus research and outbreaks
- Microtubule and mitosis dynamics
- Genetics, Bioinformatics, and Biomedical Research
- Hormonal and reproductive studies
- Contact Dermatitis and Allergies
- Microplastics and Plastic Pollution
- Oil Spill Detection and Mitigation
- Immune Cell Function and Interaction
- Isotope Analysis in Ecology
- Antimicrobial agents and applications
- Water Treatment and Disinfection
- Coastal and Marine Management
- Dye analysis and toxicity
- Immune Response and Inflammation
- Erythrocyte Function and Pathophysiology
- Immune cells in cancer
- Adipose Tissue and Metabolism
- Chemistry and Chemical Engineering
National Institute of Environmental Health Sciences
2022-2025
National Institutes of Health
2022-2025
Duke University
2020-2023
Research Triangle Park Foundation
2023
Durham Technical Community College
2021
Background: Modern chemical toxicology is facing a growing need to Reduce, Refine, and Replace animal tests (Russell 1959) for hazard identification. The most common type of assays acute toxicity assessment chemicals used as pesticides, pharmaceuticals, or in cosmetic products known “6-pack” battery tests, including three topical (skin sensitization, skin irritation corrosion, eye corrosion) systemic (acute oral toxicity, inhalation dermal toxicity) end points. Methods: We compiled, curated,...
Arginase 1 (Arg1), the enzyme catalyzing conversion of arginine to ornithine, is a hallmark IL-10-producing immunoregulatory M2 macrophages. However, its expression in T cells disputed. Here, we demonstrate that induction Arg1 key feature lung CD4+ during mouse vivo influenza infection. Conditional ablation accelerated both virus-specific helper (Th1) effector responses and resolution, resulting efficient viral clearance reduced pathology. Using unbiased transcriptomics metabolomics, found...
Abstract Even though raw mass spectrometry data is information rich, the vast majority of underutilized. The ability to interrogate these rich datasets handicapped by limited capability and flexibility existing software. We introduce Mass Spec Query Language (MassQL) that addresses issues enabling an expressive set patterns be queried directly from data. MassQL open-source query language for flexible spectrometer manufacturer-independent mining MS envision flexibility, scalability, ease use...
A comprehensive, non-targeted analysis of polar organic pollutants using high resolution/accurate mass (HR/AM) spectrometry approaches has been applied to water samples from San Francisco (SF) Bay, a major urban estuary on the western coast United States, assess occurrence emerging contaminants and inform future monitoring management activities. Polar Organic Chemical Integrative Samplers (POCIS) were deployed selectively evaluate influence three contaminant pathways: stormwater runoff (San...
Tumor dissemination is increasingly recognized to begin early in tumor development. Although most of these disseminated cells are cleared, some survive and persist below clinical detection, acting as reservoirs for metastatic relapse. Metastatic often rely on interactions with local stromal support their colonization. In this study, we propose that pericyte-tumor cell promote dormancy induction the lung, enhancing (DTC) persistence. Extravital imaging demonstrated DTCs interact pericytes...
Ethoxylated surfactants are ubiquitous organic environmental contaminants that have received continued attention over the past several decades, particularly as manufacturing rates increase worldwide and toxicity concerns grow regarding alcohol ethoxylates. Presence of these compounds in surface water has been considered primarily result contaminated wastewater effluent by ethoxylated surfactant degradates; a result, monitoring focused on small subset short-chain ethoxylates receiving waters....
Feature finding is a common way to process untargeted mass spectrometry (MS) data obtain list of chemicals present in sample. Most feature algorithms naïvely search for patterns unique descriptors (e.g., m/z, retention time, and mobility) provide unannotated features. There need solutions processing MS data, independent chemical or origin, assess features based on measurement quality with the aim improving interpretation. Here, we report signal response evaluation as method by which...
<p><a>Since 2009, animal testing for cosmetic products has been prohibited in Europe, and 2016, US EPA announced their intent to modernize the so-called "6-pack" of acute toxicity tests (acute oral toxicity, dermal inhalation skin irritation corrosion, eye sensitization) expand acceptance alternative methods reduce pesticides. We have compiled, curated, integrated largest publicly available dataset developed an ensemble QSAR models all six endpoints. All were validated according...
Since 2009, animal testing for cosmetic products has been prohibited in Europe, and 2016, US EPA announced their intent to modernize the so-called "6-pack" of acute toxicity tests (acute oral toxicity, dermal inhalation skin irritation corrosion, eye sensitization) expand acceptance alternative methods reduce pesticides. We have compiled, curated, integrated largest publicly available dataset developed an ensemble QSAR models all six endpoints. All were validated according OECD principles...
Abstract Context Pubertal girls with higher total body fat (TBF) demonstrate androgen levels. The cause of this association is unknown but hypothesized to relate insulin resistance. Objective This work aimed investigate the between TBF and androgens in pubertal using untargeted metabolomics. Methods Serum were determined a quantitative mass spectrometry (MS)–based assay. Metabolomic samples analyzed liquid chromatography high-resolution MS. Associations or index (BMI) z score (exposure)...
ABSTRACT Vaccinia virus (VACV) infection induces prominent changes in host cell metabolism. Little is known about the global metabolic reprogramming that takes place whole tissue during viral infection. Here, we performed a longitudinal metabolomics study VACV-infected mouse skin to investigate We assessed metabolites homogenized of ear pinnae over time presence or absence antigen-specific T cells using untargeted mass spectrometry. VACV induced several significant tissue, including levels...
Abstract Disclosure: M. Calvert: None. S. Molsberry: A.K. Jarmusch: K.E. Overdahl: N. Shaw: Introduction: The Body Weight and Puberty Study (BWPS) was a 4-year longitudinal study of 90 healthy pubertal, pre-menarchal girls consisting dual-energy x-ray absorptiometry to calculate total body fat (TBF), Tanner staging, breast ultrasound, hormone tests. We observed that with time, higher TBF demonstrated serum free testosterone androstenedione levels. cause this association is unknown but...
Abstract Disclosure: L. Levine: None. K. Overdahl: S. Molsberry: Shekhar: A. Jarmusch: J.E. Hall: Introduction: Metabolomics can elucidate metabolic responses to dietary interventions and has been used in energy restriction studies, primarily overweight/obese men women. Although the menstrual cycle affects metabolome, most intervention studies have not controlled for phase, nor they examined effect of moderate, short-term on profile healthy, normal-weight Objective: To characterize...
On August 9-10, 2023, a workshop was convened at the Pacific Northwest National Laboratory (PNNL) in Richland, WA that brought together group of internationally recognized experts metabolomics, natural products discovery, chemical ecology, and biological threat assessment, cheminformatics, computational chemistry, cloud computing, artificial intelligence, novel technology development. These were invited to assess value feasibility grand-scale project create new technologies would allow...