A5008513224- Immune cells in cancer
- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Cancer, Hypoxia, and Metabolism
- Chemokine receptors and signaling
- MicroRNA in disease regulation
- Kruppel-like factors research
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- Inflammation biomarkers and pathways
- Neonatal Respiratory Health Research
- Cytokine Signaling Pathways and Interactions
- Cancer, Stress, Anesthesia, and Immune Response
- Renal and related cancers
- Microtubule and mitosis dynamics
- Fire dynamics and safety research
- Cancer Immunotherapy and Biomarkers
- Advanced Proteomics Techniques and Applications
- IL-33, ST2, and ILC Pathways
- Viral Infections and Vectors
- Angiogenesis and VEGF in Cancer
- Barrier Structure and Function Studies
National Institutes of Health
2015-2025
National Cancer Institute
2014-2024
Center for Cancer Research
2015-2022
University of Virginia
2007-2016
Walter Reed Army Institute of Research
2006
Maine Farmland Trust
2003
J.P. Morgan
2003
Abstract Metastatic tumors have been shown to establish microenvironments in distant tissues that are permissive disseminated tumor cells. Hematopoietic cells contribute this microenvironment, yet the precise initiating events responsible for establishing pre-metastatic niche remain unclear. Here, we tracked developmental fate of hematopoietic stem and progenitor (HSPC) tumor-bearing mice. We show a primary drives expansion HSPCs within bone marrow their mobilization bloodstream. Treatment...
Smooth muscle cell (SMC) phenotypic modulation in atherosclerosis and response to PDGF vitro involves repression of differentiation marker genes increases SMC proliferation, migration, matrix synthesis. However, SMCs within atherosclerotic plaques can also express a number proinflammatory genes, cultured the inflammatory cytokine IL-1β represses gene expression induces expression. Studies herein tested hypothesis that modulates phenotype distinct state relative PDGF-DD. Genome-wide analysis...
Tumor dissemination is increasingly recognized to begin early in tumor development. Although most of these disseminated cells are cleared, some survive and persist below clinical detection, acting as reservoirs for metastatic relapse. Metastatic often rely on interactions with local stromal support their colonization. In this study, we propose that pericyte-tumor cell promote dormancy induction the lung, enhancing (DTC) persistence. Extravital imaging demonstrated DTCs interact pericytes...
The prognosis of men with moderate-grade prostate cancer is uncertain. At present, there are few if any reliable molecular markers that can distinguish tumors from those behave more aggressively. To better understand the basis human and potentially provide information toward accurate prognosis, we measured analyzed gene expression profiles 13 high- using cDNA microarrays. 136 genes was observed to differ significantly (P < 0.001) between normal one-sample t testing Wilcoxon ranking....
Peptide sequence identification using tandem mass spectroscopy remains a major challenge for complex proteomic studies. matching algorithms require the accurate determination of both and charge precursor ion accommodate uncertainties in these properties by wide tolerance testing, each spectrum, several possible candidate charges. Using data acquisition strategy that includes obtaining narrow mass-range MS(1) "zoom" scans, we describe here post-acquisition algorithm dubbed (Z) inference...
Abstract Background Xenotropic Murine leukemia virus-Related Virus (XMRV) is a γ-retrovirus initially reported to be present within familial human prostate tumors and the blood of patients with chronic fatigue syndrome. Subsequent studies however were unable replicate these findings, there now compelling evidence that virus evolved through rare retroviral recombination events in tumor cell lines established murine xenograft experiments. There also no direct XMRV infection has any functional...
Abstract Pericytes function to maintain tissue homeostasis by regulating capillary blood flow and maintaining endothelial barrier function. Pericyte dysfunction is associated with various pathologies has recently been found aid cancer progression. Despite having critical functions in health disease, pericytes remain an understudied population due a lack of model systems which accurately reflect vivo biology. In this study we developed protocol isolate culture murine lung, brain, bone, liver...
Abstract Pericytes function to maintain tissue homeostasis by regulating capillary blood flow and maintaining endothelial barrier function. Pericyte dysfunction is associated with various pathologies has recently been found aid cancer progression. Despite having critical functions in health disease, pericytes remain an understudied population due a lack of model systems which accurately reflect vivo biology. In this study we developed protocol isolate culture murine lung, brain, bone, liver...
Abstract ALK is overexpressed on the surface of neuroblastoma (NB) and associated with high risk disease. We developed a second generation CAR based monoclonal antibody against ALK. T-cells significantly delay growth human NB cell lines in murine xenograft models, but animals eventually succumb to tumors. In order understand how target antigen density limits effectiveness this CAR, we created library NALM-6 B-cell leukemias variable amounts expressed each clone. vitro, lyse expressing have...
Abstract Metastasis is the major cause of cancer death, and therapies that effectively target this process are required in order to improve patient survival. Previous work by Kaplan et al demonstrated activated resident stromal cells recruited bone marrow derived (BMDCs) form an altered microenvironment at distant sites response primary tumor, termed pre-metastatic niche, promotes metastatic progression. Current on-going studies laboratory focus on in-depth investigations role, composition...
Abstract Metastasis remains the main cause of mortality for patients with cancer. The process whereby a localized tumor becomes metastatic poorly understood. was previously thought to be late-stage, unidirectional migration cells from primary distant site, due acquired genetic mutations that conferred necessary attributes cell. However, current research demonstrates dissemination is not late phenomenon and although loss metastasis suppressor genes or gain can contribute phenotype, cross-talk...
Abstract Metastatic tumors have been shown to establish microenvironments in distant tissues that are permissive disseminated tumor cells. Hematopoietic cells essential components of this microenvironment, yet the events initiate hematopoietic response remain unclear. Further, functional contribution stem and progenitor (HSPCs) metastatic progression has not addressed. Here, we utilized two highly C57bl/6 murine cell lines capable spontaneous metastasis following orthotopic injection study...
Abstract Tumor cells disseminate from the primary tumor site early in disease process. These disseminated may remain dormant for years and later become proliferative to cause clinical recurrence morbidity. The goal of this study is better understand biological genetic mechanisms driving switch. We have developed a 3D culture system using murine mammary cell line, D2.0R. D2.0R when cultured on basement membrane extract (BME), exit dormancy supplemented with collagen-1 (col-1). characterized...
Abstract Metastasis is often considered a late stage event, although the first changes in metastatic site occur very early during localized primary tumor development. Although understanding of these initiating events limited, immune suppression plays an essential role allowing for outgrowth disseminating cells. Targeting this suppressive milieu can hold promise to effectively inhibit progression. Colony stimulating factor-one (CSF-1) overexpressed by many diverse types and induce expansion...
<p>Myeloid-derived suppressor cells localize with spontaneous metastases</p>
<p>Legends for Supplementary Figures 1-6.</p>