A5102881708- Immune Cell Function and Interaction
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Advanced battery technologies research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cholesterol and Lipid Metabolism
- Systemic Lupus Erythematosus Research
- Immunotherapy and Immune Responses
- Fatty Acid Research and Health
- Asthma and respiratory diseases
- Cytokine Signaling Pathways and Interactions
- Advanced Battery Materials and Technologies
- Protein Kinase Regulation and GTPase Signaling
- Antimicrobial Peptides and Activities
- Advancements in Battery Materials
- Cardiomyopathy and Myosin Studies
- RNA Interference and Gene Delivery
- Psoriasis: Treatment and Pathogenesis
- Atherosclerosis and Cardiovascular Diseases
- PARP inhibition in cancer therapy
- Electrostatic Discharge in Electronics
- Fibroblast Growth Factor Research
- Cell death mechanisms and regulation
- Tissue Engineering and Regenerative Medicine
- Cancer, Lipids, and Metabolism
University of Science and Technology of China
2022-2024
National Heart Lung and Blood Institute
2014-2024
National Institutes of Health
2014-2024
Collaborative Innovation Center of Chemistry for Energy Materials
2023-2024
Hefei National Center for Physical Sciences at Nanoscale
2022-2023
State Council of the People's Republic of China
2021
Tunxis Community College
2021
Hefei University of Technology
2021
Hefei University
2021
Core Laboratories (United States)
2010-2014
Reactive oxygen species (ROS) have been implicated as potential modulators of apoptosis. Conversely, experiments under hypoxic conditions suggested that apoptosis could occur in the absence ROS. We sought to determine whether a central modulator apoptosis, p53, regulates levels intracellular ROS and rise is required for induction p53-dependent transiently overexpressed wild-type using adenoviral gene transfer, identified cell types were sensitive or resistant p53-mediated Cells produced...
In a variety of non-phagocytic cell types, there is marked increase in intracellular levels reactive oxygen species (ROS), including superoxide and H2O2, after ligand stimulation. We demonstrate that NIH 3T3 cells transient expression constitutively activated forms the small GTP-binding proteins Ras or Rac1 leads to significant ROS. An ROS also demonstrated growth factor [platelet-derived (PDGF) epidermal (EGF)] cytokine [tumour necrosis factor-α (TNF-α) interleukin (IL)-1β] stimulation...
Room-temperature sodium-sulfur (RT Na-S) batteries, as promising next-generation energy storage candidates, are drawing more and attention due to the high density abundant elements reserved in earth. However, native downsides of RT Na-S batteries (i.e., enormous volume changes, polysulfide shuttle, insulation low reactivity S) impede their further application. To conquer these challenges, hierarchical porous hollow carbon polyhedrons embedded with uniform Mo2 C nanoparticles designed...
ABSTRACT Leakage of mitochondrial oxidants contributes to a variety harmful conditions ranging from neurodegenerative diseases cellular senescence. We describe here, however, physiological and heretofore unrecognized role for oxidant release. Mitochondrial metabolism pyruvate is demonstrated activate the c-Jun N-terminal kinase (JNK). This metabolite-induced rise in cytosolic JNK1 activity shown be triggered by increased release H 2 O . further demonstrate that turn, redox-dependent...
The signal transduction pathway leading to the activation of transcription factor NF-kappaB remains incompletely characterized. We demonstrate that in HeLa cells, transient expression a constitutively active mutant small GTP-binding protein rac1 (V12rac1) leads significant increase transcriptional activity. In addition, dominant-negative (N17rac1) inhibits basal and interleukin 1beta-stimulated Gel shift analysis using nuclear extract prepared from cells infected with recombinant adenovirus...
We used targeted gene disruption in mice to ablate nonmuscle myosin heavy chain B (NMHC-B), one of the two isoforms II present all vertebrate cells. Approximately 65% NMHC-B-/- embryos died prior birth, and those that were born suffered from congestive heart failure during first day. No abnormalities detected NMHC-B+/- mice. The absence NMHC-B resulted a significant increase transverse diameters cardiac myocytes 7.8 +/- 1.8 micron (right ventricle) 1.3 (left NMHC-B+/+ B+/- 14.7 1.1 13.8 2.3...
IL-21 is a pleiotropic type I cytokine that shares the common receptor γ chain and plays important roles for normal Ig production, terminal B cell differentiation to plasma cells, Th17 differentiation. elevated in several autoimmune diseases, blocking its action has attenuated disease MRL/lpr mice collagen-induced arthritis. The diabetes-associated Idd3 locus at Il2 / Il21 locus, was observed nonobese diabetic (NOD) mouse suggested contribute diabetes by augmenting T homeostatic...
Type III IFN lambdas (IFN-λ) have recently been described as important mediators of immune responses at barrier surfaces. However, their role in autoimmune diseases such systemic lupus erythematosus (SLE), a condition characterized by aberrant type I signaling, has not determined. Here, we identify nonredundant for IFN-λ dysregulation and tissue inflammation model TLR7-induced lupus. protein is increased murine receptor (Ifnlr1) deficiency significantly reduces cell activation associated...
The practical application of the room-temperature sodium-sulfur (RT Na-S) batteries is hindered by insulated sulfur, severe shuttle effect sodium polysulfides, and insufficient polysulfide conversion. Herein, on basis first principles calculations, single-atom vanadium anchored a 3D nitrogen-doped hierarchical porous carbon matrix (denoted as 3D-PNCV) designed fabricated to enhance sulfur reactivity, adsorption catalytic conversion performance polysulfide. 3D-PNCV host with abundant active V...
Potassium-ion batteries (KIBs) are promising candidates for large-scale energy storage devices due to their high density and low cost. However, the large potassium-ion radius leads its sluggish diffusion kinetics during intercalation into lattice of electrode material, resulting in pulverization poor cycle stability. Herein, vanadium trioxide anodes with different oxygen vacancy concentrations (V2O2.9, V2O2.8, V2O2.7 determined by neutron diffraction) developed KIBs. The V2O2.8 anode is...
Smooth muscle cell (SMC) proliferation and migration play pivotal roles in restenosis following angioplasty. c-myc is an immediate early response gene induced by various mitogens, several lines of evidence derived from experiments using transformed or hematopoietic lines, transgenic mice, suggest its protein product plays a role numerous signaling transduction pathways, including those modulating division. We therefore reasoned that strategy employing oligodeoxynucleotides (ODNs)...
The 42 amino acid form of beta amyloid (Abeta42) plays a pivotal role in neurotoxicity and the activation mononuclear phagocytes Alzheimer's disease (AD). Our recent study revealed that FPRL1, G-protein-coupled receptor, mediates chemotactic activating effect Abeta42 on (monocytes microglia), suggesting FPRL1 may be involved proinflammatory responses AD. We investigated cellular uptake subsequent fibrillar formation by using fluorescence confocal microscopy. found upon incubation with...
Abstract Prion diseases are transmissible and fatal neurodegenerative disorders which involve infiltration activation of mononuclear phagocytes at the brain lesions. A 20-aa acid fragment human cellular prion protein, PrP106–126, was reported to mimic biological activity pathologic isoform activates phagocytes. The cell surface receptor(s) mediating PrP106–126 is unknown. In this study, we show that chemotactic for monocytes through use a G protein-coupled receptor formyl peptide...
A role for mitochondrial dysfunction has been proposed in the immune dysregulation and organ damage characteristic of systemic lupus erythematosus (SLE). Idebenone is a coenzyme Q10 synthetic quinone analog an antioxidant that used humans to treat diverse diseases which function impaired. This study was undertaken assess whether idebenone ameliorates murine models.Idebenone administered orally MRL/lpr mice at 2 different doses (1 gm/kg or 1.5 idebenone-containing diet) 8 weeks. At peak...
Room‐temperature sodium–sulfur (RT Na–S) batteries are one of the most promising large‐scale energy storage systems due to their high density and abundant Na reserve. However, main challenges poor rate performance unsatisfactory capacity ascribing sluggish conversion reaction kinetics severe shuttling effect long‐chain sodium polysulfides (NaPSs) retard practical application. An ideal RT Na–S cathode host should concurrently incorporate strong adsorption capability catalytic activity....
Arginase 1 (Arg1), the enzyme catalyzing conversion of arginine to ornithine, is a hallmark IL-10-producing immunoregulatory M2 macrophages. However, its expression in T cells disputed. Here, we demonstrate that induction Arg1 key feature lung CD4+ during mouse vivo influenza infection. Conditional ablation accelerated both virus-specific helper (Th1) effector responses and resolution, resulting efficient viral clearance reduced pathology. Using unbiased transcriptomics metabolomics, found...
Formation of a bacterial biofilm within the endotracheal tube (ETT) after tracheal intubation is rapid and represents ready source lung colonization. The authors investigated colonization ventilator circuit, ETT, lungs when ETT was coated with silver-sulfadiazine chlorhexidine in polyurethane, using no bacterial/viral filter attached to ETT.Sixteen sheep were randomized into two groups. Eight intubated standard (control group), eight (study group). Animals mechanically ventilated for 24 h....
Compared to basic fibroblast growth factor (bFGF), a widely distributed, broad spectrum mitogen and mesoderm inducer, acidic (aFGF) is reported have an essentially neural distribution be undetectable in the early embryo. In present investigation, we used immunoblotting immunochemistry assess cellular tissue distributions of aFGF bFGF 11-20-d rat embryos. Immunoblotting crude heparin-bound embryo extracts revealed faint bands at expected 17-18-kD predominant apparent molecular mass 26 28-kD...
Because cytomegalovirus (CMV) may contribute to restenosis and atherosclerosis because smooth muscle cells (SMCs) are involved in these disease processes, we examined CMV-SMC interactions. Using confocal microscopy identify a redox-sensitive fluorescent marker, found that CMV infection of SMCs generates intracellular reactive oxygen intermediates (ROIs). also activated nuclear factor κB (NFκB), cellular transcription factor, as demonstrated by increased NFκB binding DNA (electrophoretic...
Evidence suggests a possible role for human cytomegalovirus (HCMV) in the development of arteriosclerosis. One earliest events plaque formation is accumulation lipid-laden foam cells, derived from macrophages and smooth muscle cells (SMCs). The lipid that occurs depends upon uptake oxidized LDL (Ox-LDL), process which scavenger receptor (SR) has been postulated to play an important role. We therefore examined effects HCMV on this process. demonstrate infection SMCs increases modified...
Blockade of angiotensin II AT1 receptors in cerebral microvessels protects against brain ischemia and inflammation. In this study, we tried to clarify the presence regulation local renin-angiotensin system (RAS) hypertension.Spontaneously hypertensive rats (SHR) Wistar Kyoto (WKY) controls were treated with an receptor antagonist (candesartan, 0.3 mg/kg per day) via subcutaneous osmotic minipumps for 4 weeks. The expression localization RAS components effect blockade assessed by Affymetrix...
Rationale: Distinct sets of corticosteroid-unresponsive genes modulate disease severity in asthma.Objectives: To identify that provide new insights into pathogenesis and asthma therapeutics.Methods: Experimental murine was induced by nasal administration house dust mite for 5 days per week. Dexamethasone apolipoprotein E (apo E) mimetic peptides were administered via osmotic minipumps.Measurements Main Results: Genome-wide expression profiling the lung transcriptome a mite–induced model...
Abstract New treatment approaches are needed for patients with asthma. Apolipoprotein A-I (apoA-I), the major structural protein of high-density lipoproteins, mediates reverse cholesterol transport and has atheroprotective anti-inflammatory effects. In this study, we hypothesized that an apoA-I mimetic peptide might be effective at inhibiting asthmatic airway inflammation. A 5A peptide, which is a synthetic, bihelical mimetic, was administered to wild-type A/J mice via osmotic mini-pump...