A5058727059- S100 Proteins and Annexins
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
- Chemokine receptors and signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Immunotherapy and Immune Responses
- Alzheimer's disease research and treatments
- Wound Healing and Treatments
- Pancreatic function and diabetes
- Peptidase Inhibition and Analysis
- Adipose Tissue and Metabolism
- Computational Drug Discovery Methods
- 3D Printing in Biomedical Research
- HIV Research and Treatment
- Bone Metabolism and Diseases
- MicroRNA in disease regulation
- Protease and Inhibitor Mechanisms
- Cancer, Stress, Anesthesia, and Immune Response
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- interferon and immune responses
- Pancreatitis Pathology and Treatment
- Neuropeptides and Animal Physiology
- Wnt/β-catenin signaling in development and cancer
- Adipokines, Inflammation, and Metabolic Diseases
- Sirtuins and Resveratrol in Medicine
Jilin University
2025
University of Chinese Academy of Sciences
2012-2024
Shanghai Institute of Nutrition and Health
2019-2024
Ruihua Affiliated Hospital of Soochow University
2017-2024
China Southern Power Grid (China)
2024
Soochow University
2017-2024
Deleted Institution
2016-2022
Shanghai Institutes for Biological Sciences
2011-2020
Yangzhou University
2020
Chinese Academy of Sciences
2010-2019
Amyloid  (A) is a major contributor to the pathogenesis of Alzheimer's disease (AD).Although A has been reported be directly neurotoxic, it also causes indirect neuronal damage by activating mononuclear phagocytes (microglia) that accumulate in and around senile plaques.In this study, we show 42 amino acid form amyloid peptide, , chemotactic agonist for seven-transmembrane, G-protein-coupled receptor named FPR-Like-1 (FPRL1), which expressed on human phagocytes.Moreover, FPRL1 at high...
Abstract Alzheimer’s disease (AD) is characterized by overproduction of β amyloid peptides in the brain with progressive loss neuronal cells. The 42-aa form peptide (Aβ42) implied as a major causative factor, because it toxic to neurons and elicits inflammatory responses activating microglial Despite Aβ42, AD tissue also generates protective factor(s) that may antagonize neurodestructive effect Aβ42. Humanin gene cloned from an apparently normal region encodes 24-aa peptide. Both secreted...
Abstract Background Inflammatory responses in the CNS mediated by activated glial cells play an important role host-defense but are also involved development of neurodegenerative diseases. Resveratrol is a natural polyphenolic compound that has cardioprotective, anticancer and anti-inflammatory properties. We investigated capacity resveratrol to protect microglia astrocyte from inflammatory insults explored mechanisms underlying different inhibitory effects on astrocytes. Methods A murine...
Alzheimers disease (AD) is characterized by amyloid plaques and neurofibrillary tangles associated with chronic inflammation. APPswe/PS1dE9 an AD mouse model bearing mutant transgenes of precursor protein presenilin-1. Amyloid deposition present in this at early stage life. However, the progression inflammation its relationship have not been characterized. Here we showed that were 4 months age increased age. CD11b-positive microglia clusters appeared hippocampus neocortex Clustered glial...
The endoplasmic reticulum quality control protein activating transcription factor 6 (ATF6) has emerged as a novel metabolic regulator. Here, we show that adenovirus-mediated overexpression of the dominant-negative form ATF6 (dnATF6) increases susceptibility to develop hepatic steatosis in diet-induced insulin-resistant mice and fasted mice. Overexpression dnATF6 or small interfering RNA-mediated knockdown decreases transcriptional activity peroxisome proliferator-activated receptor α...
Identifying functional enzymes for the catalysis of specific biochemical reactions is a major bottleneck in de novo design biosynthesis and biodegradation pathways. Conventional methods based on microbial screening metagenomics require long verification periods incur high experimental costs; recent data-driven apply only to few common substrates. To enable rapid high-throughput identification complex less-studied substrates, we propose robust enzyme's substrate promiscuity prediction model...
Trp-Lys-Tyr-Val-D-Met (WKYMVm) is a synthetic leukocyte-activating peptide postulated to use seven-transmembrane, G protein-coupled receptor(s). In the study characterize receptor(s) for WKYMVm, we found that this induced marked chemotaxis and calcium flux in human phagocytes. The signaling by WKYMVm phagocytes was attenuated high concentrations of bacterial chemotactic fMLP, suggesting might fMLP. This hypothesis tested using cells over expressing genes encoding two seven-transmembrane...
The 42 amino acid form of beta amyloid (Abeta42) plays a pivotal role in neurotoxicity and the activation mononuclear phagocytes Alzheimer's disease (AD). Our recent study revealed that FPRL1, G-protein-coupled receptor, mediates chemotactic activating effect Abeta42 on (monocytes microglia), suggesting FPRL1 may be involved proinflammatory responses AD. We investigated cellular uptake subsequent fibrillar formation by using fluorescence confocal microscopy. found upon incubation with...
Abstract Prion diseases are transmissible and fatal neurodegenerative disorders which involve infiltration activation of mononuclear phagocytes at the brain lesions. A 20-aa acid fragment human cellular prion protein, PrP106–126, was reported to mimic biological activity pathologic isoform activates phagocytes. The cell surface receptor(s) mediating PrP106–126 is unknown. In this study, we show that chemotactic for monocytes through use a G protein-coupled receptor formyl peptide...
The formylpeptide receptor (FPR) is a G-protein-coupled (GPCR) that mediates chemotaxis of phagocytic leukocytes induced by bacterial peptide N-formyl-methionyl-leucyl-phenylalanine (fMLF). We previously showed selected human glioma cell lines also express functional FPR. therefore investigated the relationship between FPR expression and biologic behavior cells.Expression function in glioblastoma line U-87 were examined reverse transcription-polymerase chain reaction (RT-PCR) assays,...
Locus ceruleus (LC) is the main subcortical site of norepinephrine synthesis. In Alzheimer's disease (AD) patients and rodent models, degeneration LC neurons reduced levels in projection areas are significantly correlated with increase amyloid plaques, neurofibrillary tangles, severity dementia. Activated microglia play a pivotal role progression AD by either clearing β peptide (Aβ) deposits through uptake Aβ or releasing cytotoxic substances proinflammatory cytokines. Here, we investigated...
Abstract The formylpeptide receptor-like 1, now officially termed FPR2, in human and its mouse homolog mFPR2 mediate leukocyte migration response to agonists associated with inflammation immune responses. To clarify the vivo role of receptor, we generated mice deficient mFPR2. mFPR2−/− showed markedly reduced severity OVA/alum-induced allergic airway inflammation. This was diminished recruitment CD11c+ dendritic cells into mucosa secondary lymphoid organs, as well production Type 2 cytokines...
Commensal bacteria and their products provide beneficial effects to the mammalian gut by stimulating epithelial cell turnover enhancing wound healing, without activating overt inflammation. We hypothesized that N-formylpeptide receptors, which bind bacterial N-formylpeptides are expressed intestinal cells, may contribute these processes. Here we report formylpeptide receptor-2 (FPR2), show is on apical lateral membranes of colonic crypt mediates N-formylpeptide–dependent proliferation...
Summary Introduction Hyperglycemia‐induced oxidative stress has been implicated in diabetic vascular complications which NADPH oxidase is a major source of reactive oxygen species ( ROS ) generation. Resveratrol naturally occurring polyphenol, vasoprotective effects animal models and inhibits high glucose HG )–induced endothelial cells. Aims We aimed to examine whether ‐induced activation production contribute glucotoxicity cells the effect resveratrol on glucotoxicity. Results Using murine...
Amyloid-β (Aβ), a natural product of cell metabolism, plays key role in the pathogenesis Alzheimer's disease (AD). Epidemiological studies indicate patients with AD have an increased risk developing type 2 diabetes mellitus (T2DM). Aβ can induce insulin resistance cultured hepatocytes by activating JAK2/STAT3/SOCS-1 signaling pathway. Amyloid precursor protein and presenilin 1 double-transgenic mouse models circulating level show impaired glucose/insulin tolerance hepatic resistance....
Wound healing is a multi-phased pathophysiological process requiring chemoattractant receptor-dependent accumulation of myeloid cells in the lesion. Two G protein-coupled formylpeptide receptors Fpr1 and Fpr2 mediate rapid neutrophil infiltration liver Listeria-infected mice by sensing pathogen-derived chemotactic ligands. These also recognize host-derived peptides inflammation injury. Here we report capacity Fprs to promote sterile skin wound initiating infiltration. We found that normal...
Obesity and related inflammation are critical for the pathogenesis of insulin resistance, but underlying mechanisms not fully understood. Formyl peptide receptor 2 (FPR2) plays important roles in host immune responses inflammation-related diseases. We found that Fpr2 expression was elevated white adipose tissue high-fat diet (HFD)–induced obese mice db/db mice. The systemic deletion alleviated HFD-induced obesity, hyperglycemia, hyperlipidemia, hepatic steatosis. Furthermore, HFD-fed body...
Abstract Receptors for the bacterial chemotactic peptide fMLP are implicated in inflammation and host defense against microbial infection. We investigated expression function of fMLPR microglial cells, which share characteristics mononuclear phagocytes play an important role proinflammatory responses CNS. The genes encoding formyl receptor (FPR)1 FPR2, high- low-affinity fMLPR, was detected a murine cell line N9, but these cells did not respond to agonists known receptors. N9 incubated with...
Background Under stress, AMP-activated protein kinase (AMPK) plays a central role in energy balance, and the heat shock response is protective mechanism for cell survival. The relationship between AMPK activity (HSP) expression under stress unclear. Methodology/Principal Findings We found that induced dephosphorylation of AMPKα subunit (AMPKα) various types from human rodent. In HepG2 cells, turn caused acetyl-CoA carboxylase upregulation phosphoenolpyruvate carboxykinase, two downstream...