A5021394893- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Immunotherapy and Immune Responses
- Acute Lymphoblastic Leukemia research
- IL-33, ST2, and ILC Pathways
- Monoclonal and Polyclonal Antibodies Research
- Cell death mechanisms and regulation
- Lymphoma Diagnosis and Treatment
- NF-κB Signaling Pathways
- Immunodeficiency and Autoimmune Disorders
- T-cell and Retrovirus Studies
- Cancer-related Molecular Pathways
- Glycosylation and Glycoproteins Research
- Psoriasis: Treatment and Pathogenesis
- Cell Adhesion Molecules Research
- Virus-based gene therapy research
- Microscopic Colitis
- Chemokine receptors and signaling
- interferon and immune responses
- Whipple's Disease and Interleukins
- Neuropeptides and Animal Physiology
- Immune cells in cancer
National Cancer Institute
2015-2024
Center for Cancer Research
2012-2024
National Institutes of Health
2011-2023
Frederick National Laboratory for Cancer Research
2003-2023
National Cancer Institute
1998-2016
National Cancer Centre Japan
2015
Office of Extramural Research
2013-2014
Leidos (United States)
2013
Logistics Management Institute (United States)
1996-2008
Ludlum Measurements (United States)
2008
Cytokines that use the common gamma chain γc are critical for lymphoid development and function. Mutations of IL-7 receptor, γc, or its associated kinase, Jak3, major cause human severe combined immunodeficiency. Although activated by IL-7, Stat5a/b (Stat, signal transducer activator transcription) have been thought to play limited roles in development. However, we now show mice completely deficient severely impaired differentiation. Absence Stat5 also abrogates T cell receptor γ...
A defining pathophysiologic feature of sepsis is profound apoptosis-induced death and depletion CD4+ CD8+ T cells. Interleukin-7 (IL-7) an antiapoptotic common γ-chain cytokine that essential for lymphocyte proliferation survival. Clinical trials IL-7 in over 390 oncologic lymphopenic patients showed was safe, invariably increased counts, improved immunity.We conducted a prospective, randomized, double-blind, placebo-controlled trial recombinant human (CYT107) with septic shock severe...
Neutrophils are a vital component of immune protection, yet in cancer they may promote tumour progression, partly by generating reactive oxygen species (ROS) that disrupts lymphocyte functions. Metabolically, neutrophils often discounted as purely glycolytic. Here we show immature, c-Kit+ subsets can engage oxidative mitochondrial metabolism. With limited glucose supply, use fatty acid oxidation to support NADPH oxidase-dependent ROS production. In 4T1 tumour-bearing mice, fitness is...
Epidemiological studies indicate that the use of artificial sweeteners doubles risk for Crohn's disease (CD). Herein, we experimentally quantified impact 6-week supplementation with a commercial sweetener (Splenda; ingredients sucralose maltodextrin, 1:99, w/w) on both severity CD-like ileitis and intestinal microbiome alterations using SAMP1/YitFc (SAMP) mice.Metagenomic shotgun DNA sequencing was first used to characterize ileitis-prone SAMP mice. Then, 16S rRNA sequencing, quantitative...
Group 3 innate lymphoid cells (ILC3s) are immune effectors that contribute to host defense. Whether ILC3 functions stably modified after pathogen encounter is unknown. Here, we assess the impact of a time-restricted enterobacterial challenge long-term activation in mice. We found intestinal ILC3s persist for months an activated state exposure
Interleukin-1 (IL-1) is a major regulator of inflammation and immunity. IL-1 induces T lymphocyte growth by acting as second signal (together with antigen) in enhancing the production interleukin-2 (IL-2). An IL-1-responsive element promoter region human IL-2 gene was similar to binding site for transcription factor AP-1. enhanced expression c- jun messenger RNA, whereas antigenic RNA fos . Thus, two components AP-1 are independently regulated may serve nuclear mediator many actions on cells.
IL-7 functions as a trophic factor during T lymphocyte development by mechanism that is partly based on the induction of Bcl-2, which protects cells from apoptosis. Here we report cytokine withdrawal activates prodeath protein Bax. On loss in dependent cell line, Bax translocated cytosol to mitochondria, where it integrated into mitochondrial membrane. This translocation was attributable conformational change itself. We show rise intracellular pH preceded and triggered conformation....
We have described an interaction between two T cells subsets that results in interference with the expression of Ly-1-, 2+ (Ly-2) cell-mediated suppression. refer to this novel immunoregulatory activity as contrasuppression. The cell responsible for induction contrasuppression (inducer cell) expresses phenotype 2+;I-J+;Qa-1+. This distinguishes it from suppressor effector which we find be I-J-2.3. An I-J+ soluble mediator contrasuppressor inducer acts on another (acceptor Ly-1+, 2+; I-J+;...
The diversity of the T cell receptor repertoire is generated by rearrangement gene elements in immature thymocytes. To identify a thymic signal that induces this rearrangement, variety agents were tested for their ability to induce beta suspensions thymocytes from mouse embryos at day 14 gestation. Of 16 tested, only interleukin-7 (IL-7) induced V(D)J and sustained expression RAG-1 RAG-2 genes, which are known control rearrangement. These data implicate IL-7, cytokine abundantly expressed...
We have examined the possibility that hyperthermia, such as occurring during fever, may benefit immune response. The effect of temperature on in vitro response unprimed murine spleen cells against antigen sheep erythrocytes was tested. Hyperthermia potently augmented plaque-forming cell Temperature-sensitive events occurred early culture period. Subsets lymphocytes were independently assessed for effects their activation and function. showed beneficial elevated probably occurs priming stage...