A5089635085- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Nail Diseases and Treatments
- Gut microbiota and health
- Plant Pathogens and Fungal Diseases
- Bladder and Urothelial Cancer Treatments
- Probiotics and Fermented Foods
- Bacterial biofilms and quorum sensing
- Pneumocystis jirovecii pneumonia detection and treatment
- Fungal Biology and Applications
- Epigenetics and DNA Methylation
- Oral microbiology and periodontitis research
- HIV/AIDS oral health manifestations
- Ocular Infections and Treatments
- Mycotoxins in Agriculture and Food
- Ocular Surface and Contact Lens
- Dermatology and Skin Diseases
- Infectious Diseases and Mycology
- Urinary Tract Infections Management
- Oral and gingival health research
- Inflammatory Bowel Disease
- Central Venous Catheters and Hemodialysis
- Clostridium difficile and Clostridium perfringens research
- Oral health in cancer treatment
- Reproductive tract infections research
Case Western Reserve University
2016-2025
University Hospitals of Cleveland
2016-2025
Temple University Hospital
2024
University School
2004-2023
Oklahoma State University Center for Health Sciences
2023
University Hospitals Cleveland Medical Center
2010-2022
Case Comprehensive Cancer Center
2022
United States Food and Drug Administration
2022
Monterey Bay Aquarium Foundation
2021
Johnson & Johnson (United States)
2019
ABSTRACT Biofilms are a protected niche for microorganisms, where they safe from antibiotic treatment and can create source of persistent infection. Using two clinically relevant Candida albicans biofilm models formed on bioprosthetic materials, we demonstrated that formation proceeds through three distinct developmental phases. These growth phases transform adherent blastospores to well-defined cellular communities encased in polysaccharide matrix. Fluorescence confocal scanning laser...
The oral microbiome–organisms residing in the cavity and their collective genome–are critical components of health disease. fungal component microbiota has not been characterized. In this study, we used a novel multitag pyrosequencing approach to characterize fungi present 20 healthy individuals, using pan-fungal internal transcribed spacer (ITS) primers. Our results revealed “basal” mycobiome profile enrolled showed that across all samples studied, contained 74 culturable 11 non-culturable...
Biofilms, likely the predominant mode of device-related microbial infection, exhibit resistance to antimicrobial agents. Evidence suggests that Candida biofilms have dramatically reduced susceptibility antifungal drugs. We examined susceptibilities albicans and parapsilosis grown on a bioprosthetic model. In addition conventional agents, we determined if new agents (triazoles, amphotericin B lipid formulations, echinocandins) activities against biofilms. also explored effects preincubation...
Denture biofilms represent a protective reservoir for oral microbes. The study of the biology Candida in these requires reliable model. A reproducible model C. albicans denture biofilm was developed and used to determine susceptibility two clinically relevant isolates against 4 antifungals. albicans, growing as biofilm, exhibited resistance amphotericin B, nystatin, chlorhexidine, fluconazole, with 50% reduction metabolic activity (50% RMA) at concentrations 8, 16, 128, > 64 μg/mL,...
Candida albicans biofilms are formed through three distinct developmental phases and associated with high fluconazole (FLU) resistance. In the present study, we used a set of isogenic strains lacking one or more drug efflux pumps Cdr1p, Cdr2p, Mdr1p to determine their role in FLU resistance biofilms. Additionally, variation sterol profile as possible mechanism was investigated. Our results indicate that parent mutant similar However, by double triple mutants were susceptible at 6 h (MIC = 64...
ABSTRACT Little is known about fungal biofilms, which may cause infection and antibiotic resistance. In this study, biofilm formation by different Candida species, particularly albicans C . parapsilosis , was evaluated using a clinically relevant model of on medical devices. biofilms were allowed to form silicone elastomer quantified tetrazolium (XTT) dry weight (DW) assays. Formed visualized fluorescence microscopy confocal scanning laser with Calcofluor White (Sigma Chemical Co., St....
Crohn's disease (CD) results from a complex interplay between host genetic factors and endogenous microbial communities. In the current study, we used Ion Torrent sequencing to characterize gut bacterial microbiota (bacteriome) fungal community (mycobiome) in patients with CD their nondiseased first-degree relatives (NCDR) 9 familial clusters living northern France-Belgium healthy individuals 4 families same area (non-CD unrelated [NCDU]). Principal component, diversity, abundance analyses...
Oral microbiota contribute to health and disease, their disruption may influence the course of oral diseases. Here, we used pyrosequencing characterize bacteriome mycobiome 12 HIV-infected patients matched uninfected controls. The number bacterial fungal genera in individuals ranged between 8–14 1–9, among participants, respectively. core (COB) comprised 14 genera, which 13 were common two groups. In contrast, (COM) differed individuals, with Candida being predominant fungus both Among...
Epidemiological studies indicate that the use of artificial sweeteners doubles risk for Crohn's disease (CD). Herein, we experimentally quantified impact 6-week supplementation with a commercial sweetener (Splenda; ingredients sucralose maltodextrin, 1:99, w/w) on both severity CD-like ileitis and intestinal microbiome alterations using SAMP1/YitFc (SAMP) mice.Metagenomic shotgun DNA sequencing was first used to characterize ileitis-prone SAMP mice. Then, 16S rRNA sequencing, quantitative...
Contaminated surfaces have been implicated as a potential route for dissemination of the emerging multidrug-resistant fungal pathogen Candida auris . In laboratory testing, C. and other species persisted 7 days on moist or dry surfaces. were recovered frequently from hospital environment, particularly Infect Control Hosp Epidemiol 2017;38:1107–1109
Contaminated surfaces are a suspected source for dissemination of the globally emerging pathogen Candida auris . In laboratory testing, sporicidal and improved hydrogen peroxide disinfectants were highly effective against C. , glabrata albicans The widely used quaternary ammonium exhibited relatively poor activity all species. Infect Control Hosp Epidemiol 2017;38:1240–1243
Abstract A growing body of literature has marked the emergence and spread antifungal resistance among species Trichophyton , most prevalent cause toenail fingernail onychomycosis in United States Europe. We review published data on rates oral species; causes methods to counteract it; vitro role topical antifungals treatment onychomycosis. Antifungal against terbinafine itraconazole—the two common treatments for other superficial fungal infections caused by dermatophytes—has been detected...
We describe a case of tinea genitalis in an immunocompetent woman Pennsylvania, USA. Infection was caused by Trichophyton indotineae potentially acquired through sexual contact. The fungus resistant to terbinafine (first-line antifungal) but improved with itraconazole. Clinicians should be aware T. as potential cause antifungal-resistant genital lesions.
The human pathogenic fungus Cryptococcus neoformans secretes a phospholipase enzyme that demonstrates B (PLB), lysophospholipase hydrolase and transacylase activities. This has been postulated to be cryptococcal virulence factor. We cloned phospholipase‐encoding gene ( PLB1 ) from C. constructed plb1 mutants using targeted disruption. All three activities were markedly reduced in the compared with wild‐type parent. strains did not have any defects known phenotypes of growth at 37°C, capsule...
ABSTRACT Colorimetric tetrazolium assays are used increasingly in studies of fungi, often the absence standardization or correlation with other methods. We examined species- and strain-related metabolism Candida albicans parapsilosis by using XTT {2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide} WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphonyl)-2H-tetrazolium] found marked variations. Also, significant signal was missed...