A5013001925- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- NF-κB Signaling Pathways
- Monoclonal and Polyclonal Antibodies Research
- IL-33, ST2, and ILC Pathways
- Immune cells in cancer
- Cell Adhesion Molecules Research
- SARS-CoV-2 and COVID-19 Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Asthma and respiratory diseases
- Rheumatoid Arthritis Research and Therapies
- Systemic Lupus Erythematosus Research
- Inflammasome and immune disorders
- Mycobacterium research and diagnosis
- COVID-19 Clinical Research Studies
- vaccines and immunoinformatics approaches
- RNA and protein synthesis mechanisms
- Protein purification and stability
- Mast cells and histamine
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Tuberculosis Research and Epidemiology
Lomonosov Moscow State University
2015-2024
Engelhardt Institute of Molecular Biology
2015-2024
Sirius University of Science and Technology
2020-2024
Moscow State University
1999-2024
Charité - Universitätsmedizin Berlin
2010-2023
Genetic Technologies (Australia)
2022-2023
Russian Academy of Sciences
2005-2023
German Rheumatism Research Centre
2009-2018
N. I. Lobachevsky State University of Nizhny Novgorod
2015-2018
Leibniz Association
2011-2017
We have explored the cis-acting elements necessary for LPS-mediated activation of mouse TNF-alpha promoter by transfecting a set 5' deletion mutants linked to CAT reporter gene into primary bone marrow-derived macrophages. A major drop in inducibility LPS was seen upon region mapping between nt -655 and -451. Gel retardation assays revealed that induced appearance this several specific DNA-protein complexes sequence motifs with strong homology kappa B enhancer. Constructs containing two or...
Macrophages are strongly adapted to their tissue of residence. Yet, little is known about the cell-cell interactions that imprint tissue-specific identities macrophages in respective niches. Using conditional depletion liver Kupffer cells, we traced developmental stages monocytes differentiating into cells and mapped cellular imprinting cell identity. loss induced tumor necrosis factor (TNF)- interleukin-1 (IL-1) receptor-dependent activation stellate endothelial resulting transient...
The mechanism of tumor necrosis factor (TNF)-induced nonapoptotic cell death is largely unknown, although the TNF-induced apoptosis has been studied extensively. In wild-type mouse embryonic fibroblast cells under a caspase-inhibited condition, TNF effectively induced that morphologically resembled necrosis. this study, we utilized gene knockout fibroblasts and found receptor (TNFR) I mediates necrotic death, RIP, FADD, TRAF2 are critical components signaling cascade death. Inhibitors NF-κB...
The inflammatory cytokine TNF-α has been recognized as a critical tumor promoter, but the effector cells that mediate its action have not fully characterized. Because B regulate squamous and prostate carcinogenesis, Tnf −/− mice harbor B-cell defects, we investigated hypothesis are important for TNF-α–mediated promotion of cancer development. Using an adoptive transfer strategy 7,12-dimethylbenz[α]anthracene/terephthalic acid (DMBA/TPA) two-stage model skin found both development...
Command and Control Innate lymphoid cells are vital for the development of gut-associated tissues, maintenance epithelial barrier, protection against intestinal microbes; their dysfunction can promote immune pathology. Immunoglobulin A (IgA) production is important gut barrier composition microbiota. Through generation knockout mouse models, Kruglov et al. (p. 1243 ) were able to distinguish how soluble membrane-bound lymphotoxins expressed by innate in specifically regulate IgA thereby...
The majority of human pancreatic cancers have activating mutations in the KRAS proto-oncogene. These result increased activity NF-κB pathway and subsequent constitutive production proinflammatory cytokines. Here, we show that inhibitor κB kinase 2 (Ikk2), a component canonical signaling pathway, synergizes with basal Notch to upregulate transcription primary target genes, resulting suppression antiinflammatory protein expression promotion carcinogenesis mice. We found Kras(G12D)Pdx1-cre...
Abstract Skin stem cells can regenerate epidermal appendages; however, hair follicles (HF) lost as a result of injury are barely regenerated. Here we show that macrophages in wounds activate HF cells, leading to telogen–anagen transition (TAT) around the wound and de novo regeneration, mostly through TNF signalling. Both knockout overexpression attenuate neogenesis wounds, suggesting dose-dependent induction by TNF, which is consistent with TNF-induced AKT signalling vitro . β-catenin...
The tumor necrosis factor (TNF) family cytokines lymphotoxin (LT) α and LTβ form heterotrimers that are expressed on the surface of activated lymphocytes natural killer cells; LTα homotrimers can be secreted as well. Mice with a disrupted gene lack lymph nodes (LN), Peyer’s patches (PP), follicular dendritic cell (FDC) networks reveal profound defects splenic architecture. However, it is unclear which these abnormalities result absence in or LTαβ heterotrimers. To distinguish between two...
The formation of germinal centers (GCs) represents a crucial step in the humoral immune response. Recent studies using gene-targeted mice have revealed that cytokines tumor necrosis factor (TNF), lymphotoxin (LT) alpha, and LTbeta, as well their receptors TNF receptor p55 (TNFRp55) LTbetaR play essential roles development GCs. To establish which cell types expression LTbetaR, is required for GC formation, LTbetaR-/-, LTbeta-/-, TNF-/-, B cell-deficient (BCR-/-), wild-type were used to...
The human major histocompatibility complex (MHC) includes the closely linked genes for tumor necrosis factors alpha and beta. Their location is within chromosomal segment between HLA-DR HLA-A or centromeric of HLA-DP. This assignment based on Southern blot analysis a number different MHC deletion mutants corroborated by chromosome in situ hybridization.
Amyloid  (A) is a major contributor to the pathogenesis of Alzheimer's disease (AD).Although A has been reported be directly neurotoxic, it also causes indirect neuronal damage by activating mononuclear phagocytes (microglia) that accumulate in and around senile plaques.In this study, we show 42 amino acid form amyloid peptide, , chemotactic agonist for seven-transmembrane, G-protein-coupled receptor named FPR-Like-1 (FPRL1), which expressed on human phagocytes.Moreover, FPRL1 at high...
Chronic inflammation is known to promote cancer, suggesting that negative regulation of likely be tumor suppressive. We found p53 a general inhibitor acts as an antagonist nuclear factor kappaB (NFkappaB). first observed striking similarities in global gene expression profiles human prostate cancer cells LNCaP transduced with inhibitory genetic element or treated TNF, inhibits transcription TNF-inducible genes are largely regulated by NFkappaB. Consistently, ectopically expressed...
Analysis of E.coli chromosomes isolated under conditions similar to those used for isolation eukaryotic chromatin has shown that: 1) The proteins highly purified deoxyribonucleoprotein are mainly in addition RNA polymerase two specific histone-like apparent molecular weight 17,000 and 9,000 (proteins 1 2, respectively). 2) Proteins 2 occur approximately equal molar amounts the chromosome, their relative content corresponds one molecule protein plus per 150-200 base pairs DNA. 3) There no...