A5027608305- Alzheimer's disease research and treatments
- Advanced Electron Microscopy Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- Cardiomyopathy and Myosin Studies
- Wnt/β-catenin signaling in development and cancer
- Neuroscience and Neuropharmacology Research
- Molecular Biology Techniques and Applications
- Cardiac electrophysiology and arrhythmias
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Cardiovascular Effects of Exercise
- Endoplasmic Reticulum Stress and Disease
- Barrier Structure and Function Studies
- Cell Image Analysis Techniques
- Cellular transport and secretion
- Image Processing Techniques and Applications
- Congenital heart defects research
- Cardiac Arrhythmias and Treatments
- Immune cells in cancer
- Protein Kinase Regulation and GTPase Signaling
- 14-3-3 protein interactions
- Mitochondrial Function and Pathology
- Ion channel regulation and function
- Anodic Oxide Films and Nanostructures
- Migration, Aging, and Tourism Studies
VIB-UGent Center for Inflammation Research
2015-2023
Ghent University
2014-2023
Ablynx (Belgium)
2023
University of Tübingen
2023
Icahn School of Medicine at Mount Sinai
2020
KU Leuven
2008-2018
Vlaams Instituut voor Biotechnologie
2018
University of Nottingham
2018
Ghent University Hospital
2016
Macrophages are strongly adapted to their tissue of residence. Yet, little is known about the cell-cell interactions that imprint tissue-specific identities macrophages in respective niches. Using conditional depletion liver Kupffer cells, we traced developmental stages monocytes differentiating into cells and mapped cellular imprinting cell identity. loss induced tumor necrosis factor (TNF)- interleukin-1 (IL-1) receptor-dependent activation stellate endothelial resulting transient...
Metabolic-associated fatty liver disease (MAFLD) represents a spectrum of states ranging from simple steatosis to non-alcoholic steatohepatitis (NASH). Hepatic macrophages, specifically Kupffer cells (KCs), are suggested play important roles in the pathogenesis MAFLD through their activation, although exact played by these remain unclear. Here, we demonstrated that KCs were reduced being replaced macrophages originating bone marrow. Recruited existed two subsets with distinct activation...
The blood–CSF barrier (BCSFB) consists of a monolayer choroid plexus epithelial (CPE) cells that maintain CNS homeostasis by producing CSF and restricting the passage undesirable molecules pathogens into brain. Alzheimer's disease is most common progressive neurodegenerative disorder characterized presence amyloid β (Aβ) plaques neurofibrillary tangles in Recent research shows associated with morphological changes CPE compromised production CSF. Here, we studied direct effects Aβ on...
The physiological functions and pathological roles of the GSK3 kinases in peripheral central systems are diverse complex, therefore hard to unravel molecular detail vivo. Our assignment review discuss available data clarify actual position these pathology Alzheimer's dementia was both ambitious easy. On one hand, numerous studies isolated, recombinant or cell-based systems, which have resulted very data-sets that hardly informative for brain At other extreme, reliable relevant models role...
Summary When electron microscopy (EM) was introduced in the 1930s it gave scientists their first look into nanoworld of cells. Over last 80 years EM has vastly increased our understanding complex cellular structures that underlie diverse functions cells need to maintain life. One drawback been difficult overcome inherent lack volume information, mainly due limit on thickness sections could be viewed a transmission microscope (TEM). For many struggled achieve three‐dimensional (3D) using...
Somatic polyploidy caused by endoreplication is observed in arthropods, molluscs, and vertebrates but especially prominent higher plants, where it has been postulated to be essential for cell growth fate maintenance. However, a comprehensive understanding of the physiological significance plant endopolyploidy remained elusive. Here, we modeled experimentally verified high-resolution DNA endoploidy map developing Arabidopsis thaliana root, revealing remarkable spatiotemporal control levels...
Central nervous glycogen synthase kinase 3β (GSK3β) is implicated in a number of neuropsychiatric diseases, such as bipolar disorder, depression, schizophrenia, fragile X syndrome or anxiety disorder. Many drugs employed to treat these conditions inhibit GSK3β either directly indirectly. We studied how conditional knockout affected structural synaptic plasticity. Deletion the gene subset cortical and hippocampal neurons adult mice led reduced spine density. In vivo imaging revealed that this...
Cable bacteria are long, multicellular micro-organisms that capable of transporting electrons from cell to along the longitudinal axis their centimeter-long filaments. The conductive structures mediate this long-distance electron transport thought be located in envelope. Therefore, study examines detail architecture envelope cable bacterium filaments through a combination classical microscopy, cryo-based microscopy and tomography, focused ion beam-scanning atomic force microscopy. We...
Pericytes and endothelial cells share membranous interdigitations called “peg-and-socket” interactions that facilitate their adhesion biochemical crosstalk during vascular homeostasis. However, the morphology distribution of these ultrastructures have remained elusive. Using a combination 3D electron microscopy techniques, we examined peg-and-socket in mouse brain capillaries. We found pegs extending from pericytes to were morphologically diverse, exhibiting claw-like morphologies at edge...
Abstract The recent advent of 3D in electron microscopy (EM) has allowed for detection nanometer resolution structures. This caused an explosion dataset size, necessitating the development automated workflows. Moreover, large EM datasets typically require hours to days be acquired and accelerated imaging results noisy data. Advanced denoising techniques can alleviate this, but tend less accessible community due low-level programming environments, complex parameter tuning or a computational...
Determining direct synaptic connections of specific neurons in the central nervous system (CNS) is a major technical challenge neuroscience. As corollary, molecular pathways controlling developmental synaptogenesis vivo remain difficult to address. Here, we present genetic tools for efficient and versatile labeling organelles, cytoskeletal components proteins at single-neuron single-synapse resolution Drosophila mechanosensory (ms) neurons. We extended imaging analysis ultrastructural level...
Cognitive demise correlates with progressive brain tauopathy in dementing patients. Improved cognition of young Tau.P301L mice contrasts dysfunction later life and remains unexplained (Boekhoorn et al., 2006). To unravel early mechanisms, we composed a correlative time line clinical symptoms, cognitive defects, biochemical pathological traits, including comprehensive analysis dendritic spines specified regions the cortex hippocampus adult mice. Remarkably, have not more, but more mature than...
The stratum lacunosum moleculare (SLM) is the connection hub between entorhinal cortex and hippocampus, two brain regions that are most vulnerable in Alzheimer's disease. We recently identified a specific synaptic deficit of Nectin-3 transgenic models for tauopathy. Here we defined cognitive impairment electrophysiological problems SLM Tau.P301L mice, which corroborated structural defects synapses dendritic spines. Reduced diffusion DiI from ERC to hippocampus indicated defective myelinated...
The endoplasmic reticulum (ER) is a complex network of sheets and tubules that continuously remodeled. relevance this membrane dynamics underscored by the fact mutations in atlastins (ATLs), ER fusion proteins mammals, cause neurodegeneration. How defects process disrupt neuronal homeostasis unclear. Using electron microscopy (EM) volume reconstruction transfected cells, neurons, patient fibroblasts, we show hereditary sensory autonomic neuropathy (HSAN)-causing ATL3 mutants promote aberrant...
Niemann-Pick type C (NPC) disease, sometimes called childhood Alzheimer's, is a rare neurovisceral lipid storage disease with progressive neurodegeneration leading to premature death. The caused by loss-of-function mutations in the Npc1 or Npc2 gene which both result into accumulation late endosomes and lysosomes. Since presents broad heterogenous clinical spectrum, involved mechanisms are still incompletely understood this hampers finding an effective treatment. As NPC patients, who carry...
This protocol allows for the efficient and effective imaging of cell or tissue samples in three dimensions at resolution level electron microscopy. For many years microscopy (EM) has remained an inherently two-dimensional technique. With advent serial scanning microscope techniques (volume EM), using either integrated microtome focused ion beam to slice then view embedded tissues, third dimension becomes easily accessible. Serial block face (SBF-SEM) uses ultramicrotome enclosed SEM chamber....