A5043807997- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Barrier Structure and Function Studies
- Immune Response and Inflammation
- Liver Disease Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Liver Disease and Transplantation
- Protease and Inhibitor Mechanisms
- MicroRNA in disease regulation
- Tryptophan and brain disorders
- Connexins and lens biology
- S100 Proteins and Annexins
- Immune cells in cancer
- Virus-based gene therapy research
- Cytokine Signaling Pathways and Interactions
- Circular RNAs in diseases
- Cancer-related gene regulation
- Immunotherapy and Immune Responses
- Helicobacter pylori-related gastroenterology studies
- Inflammation biomarkers and pathways
- Mitochondrial Function and Pathology
- Bacterial Infections and Vaccines
- Monoclonal and Polyclonal Antibodies Research
Ghent University
2016-2025
VIB-UGent Center for Inflammation Research
2016-2025
Ghent University Hospital
2008-2024
Neuroelectronics Research Flanders
2024
KU Leuven
2023-2024
Vlaams Instituut voor Biotechnologie
2012-2024
VIB-KU Leuven Center for Brain & Disease Research
2023
Karolinska Institutet
2023
Agency for Science, Technology and Research
2020
Genome Institute of Singapore
2020
Nonviral gene complexes can enter mammalian cells through different endocytic pathways. For efficient optimization of the carrier it is important to profile its cellular uptake, because this largely determines intracellular processing and subsequent transfection efficiency. Most current information on uptake these gene-delivery vehicles based data following use chemical inhibitors Here, we have performed a detailed characterization four commonly used endocytosis [chlorpromazine, genistein,...
According to the neurovascular hypothesis, impairment of low-density lipoprotein receptor–related protein-1 (LRP1) in brain capillaries blood-brain barrier (BBB) contributes neurotoxic amyloid-β (Aβ) accumulation and drives Alzheimer's disease (AD) pathology. However, due conflicting reports on involvement LRP1 Aβ transport expression endothelium, role at BBB is uncertain. As global Lrp1 deletion mice lethal, appropriate models study function are lacking. Moreover, relevance systemic...
Intracellular uptake and degradation of polyelectrolyte capsules is reported in this work. Two types intracellular degradable are described: enzyme-sensitive hydrolysis- sensitive (see figure). The results may be a significant step towards the biomedical application capsules.
Research Article5 September 2016Open Access Transparent process Identification of a novel mechanism blood–brain communication during peripheral inflammation via choroid plexus-derived extracellular vesicles Sriram Balusu Inflammation Center, VIB, Ghent, Belgium Department Biomedical Molecular Biology, Ghent University, Medical Protein Research, Biochemistry, Search for more papers by this author Elien Van Wonterghem Riet De Rycke Koen Raemdonck Laboratory General Biochemistry and Physical...
ABSTRACT Paracrine and endocrine roles have increasingly been ascribed to extracellular vesicles (EVs) generated by multicellular organisms. Central the biogenesis, content, function of EVs are their delimiting lipid bilayer membranes. To evaluate research progress on membranes EVs, International Society for Extracellular Vesicles (ISEV) conducted a workshop in March 2018 Baltimore, Maryland, USA, bringing together key opinion leaders hands‐on researchers who were selected basis submitted...
Microglia, the mononuclear phagocytes of central nervous system (CNS), are important for maintenance CNS homeostasis, but also critically contribute to pathology. Here we demonstrate that nuclear factor kappa B (NF-κB) regulatory protein A20 is crucial in regulating microglia activation during homeostasis and In mice, deletion increases microglial cell number affects regulation neuronal synaptic function. Administration a sublethal dose lipopolysaccharide induces massive activation,...
The ability of immune-modulating biologics to prevent and reverse pathology has transformed recent clinical practice. Full utility in the neuroinflammation space, however, requires identification both effective targets for local immune modulation a delivery system capable crossing blood-brain barrier. characterization small population regulatory T (Treg) cells resident brain presents one such potential therapeutic target. Here, we identified interleukin 2 (IL-2) levels as limiting factor...
Abstract The gut microbiota represents a diverse and dynamic population of microorganisms that can influence the health host. Increasing evidence supports role as key player in pathogenesis neurodegenerative diseases, including Alzheimer's disease (AD). Unfortunately, mechanisms behind interplay between pathogens AD are still elusive. It is known bacteria‐derived outer membrane vesicles (OMVs) act natural carriers virulence factors central players bacteria. Helicobacter pylori ( H. ) common...
Abstract Accumulating evidence indicates that gut microbiota dysbiosis is associated with increased blood–brain barrier (BBB) permeability and contributes to Alzheimer's disease (AD) pathogenesis. In contrast, the influence of on blood‐cerebrospinal fluid (CSF) has not yet been studied. Here, we report mice lacking display blood‐CSF disorganized tight junctions (TJs), which can be rescued by recolonization or supplementation short‐chain fatty acids (SCFAs). Our data reveal important only for...
BackgroundDysregulation of the gut microbiome has been implicated in Parkinson's disease (PD). This study aimed to evaluate clinical effects and safety a single faecal microbiota transplantation (FMT) patients with early-stage PD.MethodsThe GUT-PARFECT trial, single-centre randomised, double-blind, placebo-controlled trial was conducted at Ghent University Hospital between December 01, 2020 12, 2022. Participants (aged 50–65 years, Hoehn Yahr stage 2) were randomly assigned receive...
Understanding cellular uptake and intracellular processing of nonviral gene delivery systems is a key aspect in developing more efficient vectors. In this study, the impact clathrin- caveolae/lipid-raft-dependent endocytosis on cell entry overall transfection efficiency polyethylenimine (PEI) polyplexes was evaluated. Most remarkably, internalization pathway mediating successful depended both type polyplex applied. Colocalization studies with transferrin cholera toxin B revealed that at...
Ultrasound in combination with microbubbles has recently been considered by gene delivery scientists to be an interesting approach enhance transfer into cells. Its low toxicity and simplicity apply vivo without major complications make this technology (sonoporation) especially attractive. Sonoporation of DNA evaluated the injection free plasmid (pDNA) together (as used diagnostic imaging) bloodstream. However, gene-transfer efficiency these experiments remained rather low. Both enzymatic...
The blood–CSF barrier (BCSFB) consists of a monolayer choroid plexus epithelial (CPE) cells that maintain CNS homeostasis by producing CSF and restricting the passage undesirable molecules pathogens into brain. Alzheimer's disease is most common progressive neurodegenerative disorder characterized presence amyloid β (Aβ) plaques neurofibrillary tangles in Recent research shows associated with morphological changes CPE compromised production CSF. Here, we studied direct effects Aβ on...