Kenneth W. Witwer
A5078835957- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Circular RNAs in diseases
- HIV Research and Treatment
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- RNA modifications and cancer
- interferon and immune responses
- Viral Infections and Vectors
- Reproductive System and Pregnancy
- Neuroinflammation and Neurodegeneration Mechanisms
- HIV-related health complications and treatments
- Complement system in diseases
- Long-Term Effects of COVID-19
- Molecular Biology Techniques and Applications
- Phagocytosis and Immune Regulation
- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- Cytomegalovirus and herpesvirus research
- Mesenchymal stem cell research
- HIV/AIDS Research and Interventions
- Telomeres, Telomerase, and Senescence
- Bacterial Infections and Vaccines
Johns Hopkins Medicine
2016-2025
Johns Hopkins University
2016-2025
Precision for Medicine (United States)
2020-2024
University of Baltimore
2012-2024
Michigan Science Center
2024
Foster-Miller (United States)
2023
La Trobe University
2022
Heidelberg University
2022
University of Maryland, Baltimore
2016-2022
German Cancer Research Center
2022
ABSTRACT The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions extracellular vesicles (EVs), collective term covering various subtypes cell‐released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, many other names. However, specific issues arise when working with these entities, whose size amount often make them difficult to obtain as relatively...
Secreted membrane‐enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology medicine. Importantly, it is currently technically challenging to obtain totally pure fraction free from non‐vesicular components for functional studies, therefore there need establish guidelines analyses of these reporting studies on biology....
The emergence of publications on extracellular RNA (exRNA) and vesicles (EV) has highlighted the potential these molecules vehicles as biomarkers disease therapeutic targets. These findings have created a paradigm shift, most prominently in field oncology, prompting expanded interest dedication funds for EV research. At same time, understanding subtypes, biogenesis, cargo mechanisms shuttling remains incomplete. techniques that can be harnessed to address many gaps our current knowledge were...
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of cell origin and change functions phenotypes other cells. These features indicate strong biomarker therapeutic potential have generated broad interest, as evidenced by steady year-on-year increase in numbers scientific publications about EVs. Important advances been made EV metrology understanding applying biology. However, hurdles remain to realising EVs domains ranging from basic biology clinical...
Extracellular vesicles (EVs) represent an important mode of intercellular communication. Research in this field has grown rapidly the last few years, and there is a plethora techniques for isolation characterization EVs, many which are poorly standardized. EVs heterogeneous size, origin molecular constituents, with considerable overlap size phenotype between different populations EVs. Little known about current practices isolation, purification We report here first large, detailed survey...
The release of RNA-containing extracellular vesicles (EV) into the milieu has been demonstrated in a multitude different vitro cell systems and variety body fluids. EV are limelight for their capacity to communicate genetically encoded messages other cells, suitability as candidate biomarkers diseases, use therapeutic agents. Although EV-RNA attracted enormous interest from basic researchers, clinicians, industry, we currently have limited knowledge on which mechanisms drive regulate RNA...
Small extracellular vesicles (sEVs) from mesenchymal stromal/stem cells (MSCs) are transiting rapidly towards clinical applications. However, discrepancies and controversies about the biology, functions, potency of MSC-sEVs have arisen due to several factors: diversity MSCs their preparation; various methods sEV production separation; a lack standardized quality assurance assays; limited reproducibility in vitro vivo functional assays. To address these issues, members four societies...
KEYWORDS: Nomenclatureterminologyextracellular vesicleexosomeectosomemicrovesiclemicroparticleouter membrane vesicleMISEVMISEV2018
Abstract Motivation: Extracellular vesicles (EVs) are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of vesicular nomenclatures and components, online searches for EV-related publications components currently challenging. Results: We present an improved version EVpedia, a public database EVs research. This community web portal contains identification orthologous bioinformatic tools personalized function. EVpedia includes 6879 publications, 172...
Research on extracellular vesicles (EVs) is growing exponentially due to an increasing appreciation of EVs as disease biomarkers and therapeutics, expanding number EV-containing materials under study, application new preparation, detection, cargo analysis methods. Diversity both sources methodologies imposes challenges the comparison measurement results between studies laboratories. While reference guidelines minimal requirements for EV research have achieved important objective assembling...
Abstract Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast‐growing scientific field. In the last decade urinary (uEVs) were shown to mirror molecular processes as well physiological pathological conditions kidney, urothelial prostate tissue. Therefore, several methods isolate characterize uEVs have been developed. However, methodological aspects EV separation analysis, including normalization...
Vesicles shed from astrocytes after brain trauma trigger hepatic cytokine release to mobilize the peripheral immune response.
Abstract Cells release lipid-bound extracellular vesicles (EVs; exosomes, microvesicles and apoptotic bodies) containing proteins, lipids RNAs into the circulation. Vesicles mediate intercellular communication between both neighboring distant cells. There is substantial interest in using EVs as biomarkers for age-related diseases including cancer, neurodegenerative, metabolic cardiovascular diseases. The majority of research focuses on identifying differences when comparing disease states...
ABSTRACT Paracrine and endocrine roles have increasingly been ascribed to extracellular vesicles (EVs) generated by multicellular organisms. Central the biogenesis, content, function of EVs are their delimiting lipid bilayer membranes. To evaluate research progress on membranes EVs, International Society for Extracellular Vesicles (ISEV) conducted a workshop in March 2018 Baltimore, Maryland, USA, bringing together key opinion leaders hands‐on researchers who were selected basis submitted...
Intercellular communication can be mediated by extracellular small regulatory RNAs (sRNAs). Circulating sRNAs are being intensively studied for their promising use as minimally invasive disease biomarkers. To date, most attention is centered on exosomes and microRNAs the vectors secreted species, respectively. However, this field would benefit from an increased understanding of plethora different cell types in fractions. It still not clear if specific selected secretion, or sRNA secretion...
1. 2. 3. 4. Scientific Program ISEV2018Thursday, 02 May 2018PL 01: Plenary Session 1: Autophagy, Metabolism and AgeingChairs: Juan Falcon-Perez; Andrew Hill Location: Auditorium09:30–10:30PL 1The M...