A5065525654- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Ion Transport and Channel Regulation
- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
- Renal and related cancers
- Electrolyte and hormonal disorders
- Parasites and Host Interactions
- Ion channel regulation and function
- Trace Elements in Health
- Organ Transplantation Techniques and Outcomes
- Erythrocyte Function and Pathophysiology
- Renal Transplantation Outcomes and Treatments
- Parasite Biology and Host Interactions
- Pancreatic function and diabetes
- Angiogenesis and VEGF in Cancer
- 3D Printing in Biomedical Research
- Genetic and Kidney Cyst Diseases
- Viral Infections and Vectors
- Organ Donation and Transplantation
- Salmonella and Campylobacter epidemiology
- Microbial metabolism and enzyme function
- Circular RNAs in diseases
- Cellular transport and secretion
- Tissue Engineering and Regenerative Medicine
University Medical Center Utrecht
2015-2025
Utrecht University
2004-2019
Hypertension Institute
2017
Jagiellonian University
2014
University Medical Center
2014
Pohang University of Science and Technology
2014
AGH University of Krakow
2014
Heidelberg University
2014
University Hospital Heidelberg
2014
Eindhoven University of Technology
2014
Extracellular vesicles (EVs) are membraneous released by a variety of cells into their microenvironment. Recent studies have elucidated the role EVs in intercellular communication, pathogenesis, drug, vaccine and gene-vector delivery, as possible reservoirs biomarkers. These findings generated immense interest, along with an exponential increase molecular data pertaining to EVs. Here, we describe Vesiclepedia, manually curated compendium (lipid, RNA, protein) identified different classes...
Extracellular vesicles (EVs), such as exosomes and microvesicles, are released by different cell types participate in physiological pathophysiological processes. EVs mediate intercellular communication cell‐derived extracellular signalling organelles that transmit specific information from their of origin to target cells. As a result these properties, defined may serve novel tools for various therapeutic approaches, including (a) anti‐tumour therapy, (b) pathogen vaccination, (c)...
The release of RNA-containing extracellular vesicles (EV) into the milieu has been demonstrated in a multitude different vitro cell systems and variety body fluids. EV are limelight for their capacity to communicate genetically encoded messages other cells, suitability as candidate biomarkers diseases, use therapeutic agents. Although EV-RNA attracted enormous interest from basic researchers, clinicians, industry, we currently have limited knowledge on which mechanisms drive regulate RNA...
The healthy vascular endothelium, which forms the barrier between blood and surrounding tissues, is known to efficiently respond stress signals like hypoxia inflammation by adaptation of cellular physiology secretion (soluble) growth factors cytokines. Exosomes are potent mediators intercellular communication. Their content consists RNA proteins from cell origin, thus depends on condition these cells at time exosome biogenesis. It has been suggested that exosomes protect their target through...
Small extracellular vesicles (sEVs) from mesenchymal stromal/stem cells (MSCs) are transiting rapidly towards clinical applications. However, discrepancies and controversies about the biology, functions, potency of MSC-sEVs have arisen due to several factors: diversity MSCs their preparation; various methods sEV production separation; a lack standardized quality assurance assays; limited reproducibility in vitro vivo functional assays. To address these issues, members four societies...
Abstract Motivation: Extracellular vesicles (EVs) are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of vesicular nomenclatures and components, online searches for EV-related publications components currently challenging. Results: We present an improved version EVpedia, a public database EVs research. This community web portal contains identification orthologous bioinformatic tools personalized function. EVpedia includes 6879 publications, 172...
Exosomes are small vesicles that mediate cell–cell communication. They contain proteins, lipids and RNA, evidence is accumulating these molecules specifically sorted for release via exosomes. We recently showed endothelial‐cell‐produced exosomes promote angiogenesis in vivo a RNA‐dependent manner. Recent deep sequencing studies from lymphocytic origin revealed broad spectrum of RNAs. However, selective depletion or incorporation RNA species into endothelial has not been studied extensively....
Professional antigen‐presenting cells secrete major histocompatibility complex class II (MHC II) carrying exosomes with unclear physiological function(s). Exosomes are first generated as the intraluminal vesicles (ILVs) of a specific type multivesicular body, and then secreted by fusion this compartment plasma membrane. We have previously shown that in contrast to sorting MHC at lysosomally targeted bodies, into does not rely on ubiquitination. In search for proteins drive incorporation or...
The regular workshops held by the Center for Alternatives to Animal Testing (CAAT) on biology-inspired microphysiological systems (MPS) taking place every four years, have become a reliable measure assess fundamental scientific, industrial and regulatory trends translational science in MPS-field from bird's eye view. 2023 workshop participants at that time concluded technology as used within academia has matured significantly, underlined broad use of MPS steadily increasing number high...
In renal collecting ducts, a vasopressin-induced cAMP increase results in the phosphorylation of aquaporin-2 (AQP2) water channels at Ser-256 and its redistribution from intracellular vesicles to apical membrane. Hormones that activate protein kinase C (PKC) proteins counteract this process. To determine role putative sites trafficking hormonal regulation human AQP2, three casein II (Ser-148, Ser-229, Thr-244), one PKC (Ser-231), A (Ser-256) site were altered mimic constitutively...
• Cell therapy The paracrine hypothesis Exosomes Collection CMPC secreted exosomes Conditioned medium and in vitro scratch wound assay Exosomal signalling via MMP EMMPRIN Discussion Patients suffering from heart failure as a result of myocardial infarction are need transplantation. Unfortunately the number donor hearts is very low therefore new therapies subject investigation. transplantation upon promising strategy to replace dead myocardium with viable cardiomyocytes, smooth muscle cells...
Chronic kidney disease (CKD) is a major health care problem, affecting more than 35% of the elderly population worldwide. New interventions to slow or prevent progression are urgently needed. Beneficial effects mesenchymal stem cells (MSC) have been described, however it unclear whether MSCs themselves their secretome required. We hypothesized that MSC-derived conditioned medium (CM) reduces CKD and studied functional structural in rat model established CKD. was induced by 5/6 nephrectomy...
Extracellular vesicles (EVs) released by human adipocytes or adipose tissue (AT)-explants play a role in the paracrine interaction between and macrophages, key mechanism AT inflammation, leading to metabolic complications like insulin resistance (IR) were determined.EVs from vitro differentiated AT-explants ex vivo characterized electron microscopy, Western blot, multiplex adipokine-profiling, quantified flow cytometry. Primary monocytes stimulated with EVs adipocytes, subcutaneous (SCAT)...
Abstract Exosomes are important mediators of intercellular communication. Additionally, they contain a variety components capable interacting with the extracellular matrix ( ECM ), including integrins, metalloproteinases and members immunoglobin superfamily. Despite these observations, research on exosome‐ECM interactions is limited. Here, we investigate whether exosome‐associated lysyl oxidase family member oxidase‐like 2 LOXL 2) involved in remodelling. We found that present exterior...
Abstract Small extracellular vesicles (EVs) are 50–200 nm secreted by most cells. They considered as mediators of intercellular communication, and EVs from specific cell types, in particular mesenchymal stem/stromal cells (MSCs), offer powerful therapeutic potential, can provide a novel strategy. appear promising safe (as non‐self‐replicating), eventually MSC‐derived (MSC‐EVs) may be developed to standardized, off‐the‐shelf allogeneic regenerative immunomodulatory therapeutics. Promising...
Abstract Mesenchymal stromal cell (MSC)‐derived small extracellular vesicles (sEVs) show therapeutic potential in multiple disease models, including kidney injury. Clinical translation of sEVs requires further preclinical and regulatory developments, elucidation the biodistribution mode action (MoA). Biodistribution can be determined using labelled animal models which come with ethical concerns, are time‐consuming expensive, may not well represent human physiology. We hypothesised that,...
Abstract Mesenchymal stromal cells (MSCs) are promising regenerative therapeutics that primarily exert their effects through secreted extracellular vesicles (EVs). These EVs – being small and non‐living easier to handle possess advantages over cellular products. Consequently, the therapeutic potential of MSC‐EVs is increasingly investigated. However, due variations in MSC‐EV manufacturing strategies, products should be considered as highly diverse. Moreover, diverse array EV characterisation...
Schistosoma mansoni is a parasitic worm that lives in the blood vessels of its host. We mapped S. tegumental outer-surface structure proteome by 1D SDS-PAGE and LC−MS/MS an EST-database from ongoing genome-sequencing project. identified 740 proteins which 43 were tegument-specific. Many these show no homology to any nonschistosomal protein, demonstrating schistosomal comprises specific unique proteins, likely be critical for parasite survival. Keywords: immune evasion • host-parasite...