A5031362151- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Occupational and environmental lung diseases
- T-cell and B-cell Immunology
- Medical Imaging and Pathology Studies
- Pleural and Pulmonary Diseases
- Pancreatic and Hepatic Oncology Research
- Extracellular vesicles in disease
- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Inflammatory Biomarkers in Disease Prognosis
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Lung Cancer Research Studies
- Colorectal Cancer Treatments and Studies
- Renal cell carcinoma treatment
- Phagocytosis and Immune Regulation
- Ferroptosis and cancer prognosis
- Gastrointestinal Tumor Research and Treatment
- Radiomics and Machine Learning in Medical Imaging
- Immune Response and Inflammation
- Cancer, Stress, Anesthesia, and Immune Response
- Gut microbiota and health
Institut Gustave Roussy
2016-2025
Inserm
2015-2024
Université Paris-Saclay
2013-2024
Centre National de la Recherche Scientifique
2015-2024
Universidade da Coruña
2024
Stabilité génétique et oncogenèse
2013-2023
La Ligue Contre le Cancer
2021
Biotherapy of Genetic Diseases, Inflammatory Disorders and Cancers
2021
Aix-Marseille Université
2021
Pharmac
2018-2020
Gut microbes affect immunotherapy The unleashing of antitumor T cell responses has ushered in a new era cancer treatment. Although these therapies can cause dramatic tumor regressions some patients, many patients inexplicably see no benefit. Mice have been used two studies to investigate what might be happening. Specific members the gut microbiota influence efficacy this type (see Perspective by Snyder et al. ). Vétizou found that optimal anticytotoxic lymphocyte antigen blockade required...
Systemic anticancer chemotherapy is immunosuppressive and mostly induces nonimmunogenic tumor cell death. Here, we show that even in the absence of any adjuvant, cells dying response to anthracyclins can elicit an effective antitumor immune suppresses growth inoculated tumors or leads regression established neoplasia. Although both antracyclins mitomycin C induced apoptosis with caspase activation, only anthracyclin-induced immunogenic death was immunogenic. Caspase inhibition by Z-VAD-fmk...
Extracellular vesicles (EVs), such as exosomes and microvesicles, are released by different cell types participate in physiological pathophysiological processes. EVs mediate intercellular communication cell‐derived extracellular signalling organelles that transmit specific information from their of origin to target cells. As a result these properties, defined may serve novel tools for various therapeutic approaches, including (a) anti‐tumour therapy, (b) pathogen vaccination, (c)...
BACKGROUND: DC derived-exosomes are nanomeric vesicles harboring functional MHC/peptide complexes capable of promoting T cell immune responses and tumor rejection. Here we report the feasability safety first Phase I clinical trial using autologous exosomes pulsed with MAGE 3 peptides for immunization stage III/IV melanoma patients. Secondary endpoints were monitoring outcome. PATIENTS AND METHODS: Exosomes purified from day 7 monocyte derived-DC cultures. Fifteen patients fullfilling...
Purpose: While immune checkpoint inhibitors are disrupting the management of patients with cancer, anecdotal occurrences rapid progression (i.e., hyperprogressive disease or HPD) under these agents have been described, suggesting potentially deleterious effects drugs. The prevalence, natural history, and predictive factors HPD in cancer treated by anti-PD-1/PD-L1 remain unknown.Experimental Design: Medical records from all (N = 218) prospectively Gustave Roussy within phase I clinical trials...
Tumor growth promotes the expansion of CD4+CD25+ regulatory T (T reg) cells that counteract cell–mediated immune responses. An inverse correlation between natural killer (NK) cell activation and reg in tumor-bearing patients, shown here, prompted us to address role controlling innate antitumor immunity. Our experiments indicate human expressed membrane-bound transforming factor (TGF)–β, which directly inhibited NK effector functions down-regulated NKG2D receptors on surface. Adoptive...
<h3>Importance</h3> Derived neutrophils/(leukocytes minus neutrophils) ratio (dNLR) and lactate dehydrogenase (LDH) level have been correlated with immune checkpoint inhibitor (ICI) outcomes in patients melanoma. <h3>Objective</h3> To determine whether pretreatment dNLR LDH are associated resistance to ICIs advanced non–small cell lung cancer (NSCLC). <h3>Design, Setting, Participants</h3> Multicenter retrospective study a test (n = 161) validation set 305) treated programmed death...
Dendritic cell-derived exosomes (Dex) are small extracellular vesicles secreted by viable dendritic cells. In the two phase-I trials that we conducted using first generation of Dex (IFN-γ-free) in end-stage cancer, reported exerted natural killer (NK) cell effector functions patients. A second (IFN-γ-Dex) was manufactured with aim boosting NK and T immune responses. We carried out a phase II clinical trial testing benefit IFN-γ-Dex loaded MHC class I- II-restricted cancer antigens as...
Current immunization protocols in cancer patients involve CTL-defined tumor peptides. Mature dendritic cells (DC) are the most potent APCs for priming of naive CD8(+) T cells, eventually leading to eradication. Because DC can secrete MHC class I-bearing exosomes, we addressed whether exosomes pulsed with synthetic peptides could subserve function consisting I-restricted, peptide-specific CTL vitro and vivo. The restricted by HLA-A2 molecules specific melanoma was performed: 1) using...
Dendritic cell (DC) derived-exosomes (Dex) are nanomeric vesicles harboring functional MHC/peptide complexes promoting T cell-dependent tumor rejection. In the first Phase I trial using peptide-pulsed Dex, observation of clinical regressions in absence responses prompted search for alternate effector mechanisms. Mouse studies unraveled bioactivity Dex on NK cells. Indeed, promoted an IL-15Ralpha- and NKG2D-dependent proliferation activation respectively, resulting anti-metastatic effects...
Abstract In response to some chemotherapeutic agents such as anthracyclines and oxaliplatin, cancer cells undergo immunogenic apoptosis, meaning that their corpses are engulfed by dendritic tumor cell antigens presented tumor-specific CD8+ T cells, which then control residual cells. One of the peculiarities apoptosis is early surface exposure calreticulin (CRT), a protein usually resides in lumen endoplasmic reticulum (ER). When elicited or CRT pathway activated pre-apoptotic ER stress...
Abstract Gut microbiota composition influences the clinical benefit of immune checkpoints in patients with advanced cancer but mechanisms underlying this relationship remain unclear. Molecular mechanism whereby gut responses is mainly assigned to microbial metabolites. Short-chain fatty acids (SCFA) are produced large amounts colon through bacterial fermentation dietary fiber. We evaluate mice and treated anti-CTLA-4 blocking mAbs whether SCFA levels related outcome. High blood butyrate...
Abstract Immunosuppressive cytokines subvert innate and adaptive immune responses during cancer progression. The inflammatory cytokine interleukin-18 (IL-18) is known to accumulate in patients, but its pathophysiological role remains unclear. In this study, we show that low levels of circulating IL-18, either exogenous or tumor derived, act suppress the NK cell arm immunosurveillance. IL-18 produced by cells promotes development NK-controlled metastases a PD-1–dependent manner. Accordingly,...
Therapeutic monoclonal anti-cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) antibodies are associated with immune-mediated enterocolitis. The aim of this study was to provide a detailed description entity. We included patients endoscopic signs inflammation after anti-CTLA-4 infusions for cancer treatment. Other causes enterocolitis were excluded. Clinical, biological and data recorded. A single pathologist reviewed biopsies colectomy specimens from 27 patients. Patients without...
By triggering immunogenic cell death, some anticancer compounds, including anthracyclines and oxaliplatin, elicit tumor-specific, interferon-γ–producing CD8+ αβ T lymphocytes (Tc1 CTLs) that are pivotal for an optimal therapeutic outcome. Here, we demonstrate chemotherapy induces a rapid prominent invasion of interleukin (IL)-17–producing γδ (Vγ4+ Vγ6+) (γδ T17 cells) precedes the accumulation Tc1 CTLs within tumor bed. In receptor δ−/− or Vγ4/6−/− mice, efficacy was compromised, no IL-17...