A5006675461- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Acute Myeloid Leukemia Research
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Hematopoietic Stem Cell Transplantation
- Circular RNAs in diseases
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Photodynamic Therapy Research Studies
- Immune cells in cancer
- Nanoplatforms for cancer theranostics
- Cell Adhesion Molecules Research
- Head and Neck Cancer Studies
- Occupational and environmental lung diseases
- Sphingolipid Metabolism and Signaling
- Bone health and treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Osteoarthritis Treatment and Mechanisms
- Parvovirus B19 Infection Studies
- Phagocytosis and Immune Regulation
- Liver physiology and pathology
- Renal cell carcinoma treatment
Essen University Hospital
2015-2023
University of Duisburg-Essen
2015-2023
Heinrich Heine University Düsseldorf
2009-2015
Düsseldorf University Hospital
2011-2015
Marien Hospital Düsseldorf
2011
Extracellular vesicles (EVs), such as exosomes and microvesicles, are released by different cell types participate in physiological pathophysiological processes. EVs mediate intercellular communication cell‐derived extracellular signalling organelles that transmit specific information from their of origin to target cells. As a result these properties, defined may serve novel tools for various therapeutic approaches, including (a) anti‐tumour therapy, (b) pathogen vaccination, (c)...
Extracellular vesicles (EVs) provide a complex means of intercellular signalling between cells at local and distant sites, both within different organs. According to their cell-type specific signatures, EVs can function as novel class biomarkers for variety diseases, be used drug-delivery vehicles. Furthermore, from certain cell types exert beneficial effects in regenerative medicine immune modulation. Several techniques are available harvest various body fluids or culture supernatants....
Abstract Obtained from the right cell-type, mesenchymal stromal cell (MSC)-derived small extracellular vesicles (sEVs) promote stroke recovery. Within this process, microvascular remodeling plays a central role. Herein, we evaluated effects of MSC-sEVs on proliferation, migration, and tube formation human cerebral endothelial cells (hCMEC/D3) in vitro post-ischemic angiogenesis, brain neurological recovery after middle artery occlusion (MCAO) mice. In vitro, sEVs obtained hypoxic (1% O 2 ),...
Background and Purpose— Small extracellular vesicles (sEVs) obtained from mesenchymal stromal cells (MSCs) were shown to induce neurological recovery after focal cerebral ischemia in rodents reverse postischemic lymphopenia peripheral blood. Since blood cells, especially polymorphonuclear neutrophils (PMNs), contribute ischemic brain injury, we analyzed leukocyte responses sEVs investigated the role of PMNs sEV-induced neuroprotection. Methods— Male C57Bl6/j mice exposed transient...
Extracellular vesicles (EVs) harvested from conditioned media of human mesenchymal stromal cells (MSCs) suppress acute inflammation in various disease models and promote regeneration damaged tissues. After successful treatment a patient with steroid-refractory graft-versus-host (GVHD) using EVs prepared bone marrow-derived MSCs, this study focused on improving the MSC-EV production for clinical application.
Abstract Cell culture‐conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. culturing parameters affect the biochemical composition, release possibly function CCM‐derived EVs (CCM‐EV). The CCM‐EV task force Rigor Standardization Subcommittee International Society Extracellular Vesicles aims to identify relevant cell parameters, describe their effects based on current knowledge, recommend reporting...
Abstract Small extracellular vesicles (sEVs) obtained from mesenchymal stromal cells (MSCs) promote neurological recovery after middle cerebral artery occlusion (MCAO) in young rodents. Ischemic stroke mainly affects aged humans. MSC-sEV effects on rodents had not been assessed. In a head-to-head comparison, we exposed (4–5 months) and (19–20 male Sprague–Dawley rats to permanent distal MCAO. At 24 h, 3 7 days post-stroke, vehicle or MSC-sEVs (2 × 10 6 2 MSC equivalents/kg) were...
T-cell responses contribute to the anti-tumoural effect of photodynamic therapy (PDT). For such occur, dendritic cells (DCs) have migrate tumour, take up tumour antigens and respond danger signals with maturation, before they engage in activation. Here, we studied 5-aminolevulinic acid (ALA)-mediated PDT on DCs vitro a human spheroid model glioblastoma (GB).Spheroids GB cell lines U87 U251 were treated ALA/PDT, effects attraction, uptake maturation studied. To block heat-shock protein-70...
Abstract Mesenchymal stem/stromal cells (MSCs) provide therapeutic effects in many diseases. Contrary to initial hypotheses, they act a paracrine rather than cellular manner. To this end, extracellular vesicles (EVs) have been found mediate the effects, even when harvested from MSC‐conditioned cell culture supernatants. Lacking self‐replicating activity and being so small that MSC‐EV preparations can be sterilized by filtration, EVs several advantages as agents over therapeutics. At present,...
Background: Neonatal encephalopathy caused by hypoxia-ischemia (HI) is a major cause of childhood mortality and disability. Stem cell-based regenerative therapies seem promising to prevent long-term neurological deficits. Our previous work in neonatal HI revealed an unexpected interaction between mesenchymal stem/stromal cells (MSCs) the brains' microenvironment leading altered therapeutic efficiency. MSCs are supposed mediate most their effects paracrine mode via extracellular vesicles...
Small extracellular vesicles isolated from urine (uEVs) are increasingly recognized as potential biomarkers. Meanwhile, different uEV preparation strategies exist. Conventionally, the performance of EV methods is evaluated by single particle quantification, Western blot, and electron microscopy. Recently, we introduced imaging flow cytometry (IFCM) a next-generation analysis technology. Here, analyzed samples obtained with procedures using nanoparticle tracking (NTA), semiquantitative IFCM....
Small extracellular vesicles (sEVs) obtained from mesenchymal stromal cells (MSCs) were shown to induce ischemic neuroprotection in mice by modulating the brain infiltration of leukocytes and, specifically polymorphonuclear neutrophils. So far, effects MSC-sEVs only studied young rodents. We herein examined aged mice.
Antidepressants have been reported to enhance stroke recovery independent of the presence depressive symptoms. They recently proposed exert their mood-stabilizing actions by inhibition acid sphingomyelinase (ASM), which catalyzes hydrolysis sphingomyelin ceramide. Their restorative action post-ischemia/reperfusion (I/R) still had be defined. Mice subjected middle cerebral artery occlusion or microvascular endothelial cells exposed oxygen-glucose deprivation were treated with vehicle...
Treatment with extracellular vesicles (EVs) derived from mesenchymal stem/stromal cells (MSCs) have been suggested as novel therapeutic option in acute inflammation-associated disorders due to their immune-modulatory capacities. As we previously observed differences the cytokine profile of independent MSC-EV preparations, functional preparations be considered. To evaluate capabilities specific reliable assays are required characterize functionality prior administration a patient. this end,...
Niemann–Pick type C (NPC) disease is a rare neurovisceral lipid storage with progressive neurodegeneration, leading to premature death. The caused by loss-of-function mutations either in the NPC1 or NPC2 gene which results accumulation late endosomes and lysosomes. involved mechanisms are still incompletely understood, making design of rational treatment very difficult. Since characterized peripheral inflammation neuroinflammation it shown that extracellular vesicles (EVs) obtained from...
Background aimsExtracellular vesicles (EVs) derived from human mesenchymal stromal cells (MSCs) show immunomodulatory activity in different assays both vitro and vivo. In previous work, the authors compared potential of independent MSC-EV preparations a multi-donor mixed lymphocyte reaction (mdMLR) assay an optimized steroid-refractory acute graft-versus-host disease (aGVHD) mouse model. The observed that only proportion showed capabilities demonstrated with mdMLR immunomodulating activities...
Introduction Osteoarthritis (OA) affects a large percentage of the population worldwide. Current surgical and nonsurgical concepts for treating OA only result in symptom-modifying effects. However, there is no disease-modifying therapy available. Extracellular vesicles released by mesenchymal stem/stromal cells (MSC-EV) are promising agents to positively influence joint homeostasis osteoarthritic surroundings. This pilot study aimed investigate effect characterized MSC-EVs on chondrogenesis...