A5101839295- Neonatal Respiratory Health Research
- Mesenchymal stem cell research
- Extracellular vesicles in disease
- Tissue Engineering and Regenerative Medicine
- Pregnancy and preeclampsia studies
- Congenital Diaphragmatic Hernia Studies
- Birth, Development, and Health
- Reproductive System and Pregnancy
- Preterm Birth and Chorioamnionitis
- MicroRNA in disease regulation
- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Neonatal and fetal brain pathology
- Infant Nutrition and Health
- Congenital Anomalies and Fetal Surgery
- Pluripotent Stem Cells Research
- Viral Infectious Diseases and Gene Expression in Insects
- CAR-T cell therapy research
- Respiratory Support and Mechanisms
- Microfluidic and Bio-sensing Technologies
- Connective Tissue Growth Factor Research
- Biomedical Ethics and Regulation
- Gestational Diabetes Research and Management
- Corneal Surgery and Treatments
- Organ Transplantation Techniques and Outcomes
Hudson Institute of Medical Research
2016-2025
Hudson Institute
2013-2025
Monash University
2015-2024
Australian Regenerative Medicine Institute
2014-2023
University of Wisconsin–Madison
2023
Istituti di Ricovero e Cura a Carattere Scientifico
2022
University of Pavia
2022
Westmead Hospital
2020-2022
Monash Medical Centre
2012-2020
Merck (Singapore)
2020
Idiopathic pulmonary fibrosis (IPF) is characterized by chronic inflammation, severe scarring, and stem cell senescence. Stem cell-based therapies modulate inflammatory fibrogenic pathways release of soluble factors. cell-derived extracellular vesicles should be explored as a potential therapy for IPF. Human amnion epithelial exosomes (hAEC Exo) were isolated compared against human lung fibroblasts exosomes. hAEC Exo assessed fibrosis. Exosomes evaluated their protein miRNA cargo. Direct...
Abstract Human amnion epithelial cells (hAECs) are a heterologous population positive for stem cell markers; they display multilineage differentiation potential, differentiating into of the endoderm (liver, lung epithelium), mesoderm (bone, fat), and ectoderm (neural cells). They have low immunogenic profile possess potent immunosuppressive properties. Hence, hAECs may be valuable source therapy. This unit describes an efficient effective method hAEC isolation, culture, cryopreservation that...
Human amnion epithelial cells (hAECs) have attracted recent attention as a promising source of for regenerative therapies, with reports that derived from human term possess multipotent differentiation ability, low immunogenicity, and anti-inflammatory properties. Specifically, in animal models lung disease characterized by significant loss tissue secondary to chronic inflammation fibrosis, the transplantation hAECs has been shown reduce both subsequent fibrosis. To further explore mechanisms...
Abstract Preeclampsia remains a leading cause of maternal and perinatal morbidity mortality. There have been no material advances in the treatment preeclampsia for nearly 50 years. Combining vitro studies clinical trial, we aimed to determine whether melatonin could be useful adjuvant therapy. In xanthine/xanthine oxidase (X/ XO ) placental explant model, reduced oxidative stress (8‐isoprostane) enhanced antioxidant markers (Nrf2 translocation, HO ‐1), but did not affect production...
Abstract Bronchopulmonary dysplasia (BPD) is a chronic lung disease that mainly affects premature babies who require ventilator support. The pathogenesis of BPD complex but includes vascular maldevelopment, alveolarization arrest, and inflammation. There no cure for BPD. Clinical care limited to supportive respiratory measures. A population stem-like cells derived from placental membranes, human amnion epithelial (hAECs), has shown therapeutic promise in preclinical models With view future...
Human amnion epithelial cells (hAECs) have been shown to modulate inflammation and restore normal lung structure respiratory function following bleomycin challenge in immune-competent mice. These effects are exerted despite a lack of significant engraftment hAECs, suggesting that immunomodulatory effect mechanisms at play. In this study, using the model injury, we explored interactions between hAECs macrophages. We administered 4 million intraperitoneally C57Bl6 mice 24 h challenge. Using...
Therapeutic benefits of mesenchymal stem cells (MSCs) are now widely believed to come from their paracrine signalling, i.e. secreted factors such as cytokines, chemokines, and extracellular vesicles (EVs). Cell-free therapy using EVs is an active emerging field in regenerative medicine. Typical 2D cultures on tissue culture plastic far removed the physiological environment MSCs. The application 3D cell allows MSCs adapt cellular which, turn, influences signalling activity. In this study we...
Background: Hepatic stellate cells (HSCs) are the primary collagen-secreting in liver. While HSCs major cell type involved pathogenesis of liver fibrosis, hepatic macrophages also play an important role mediating fibrogenesis and fibrosis resolution. Previously, we observed a reduction HSC activation, proliferation, collagen synthesis following exposure to human amnion epithelial (hAEC) hAEC-conditioned media (hAEC-CM). This suggested that specific factors secreted by hAEC might be effective...
Ischemic stroke is a debilitating disease for which there are currently no effective treatments besides the clot-buster, tissue plasminogen activator (t-PA), administered to less than 10% of patients due limited (4.5 h) time window efficacy. Thus, an urgent need novel therapies that can prevent or reverse effects stroke-induced brain injury. Recent encouraging reports have revealed stem cells derived from human tissue, including embryonic, induced pluripotent, neural, and mesenchymal cells,...
Multiple sclerosis (MS) is an autoimmune inflammatory disease of the central nervous system (CNS). In recent years, it has been found that cells such as human amnion epithelial (hAECs) have ability to modulate immune responses in vitro and vivo can differentiate into multiple cell lineages. Accordingly, we investigated immunoregulatory effects hAECs a potential therapy MS-like disease, EAE (experimental encephalomyelitis), mice. Using flow cytometry, phenotypic profile from different donors...
The immunomodulatory properties of human amnion epithelial cells (hAECs) have been previously described in several disease models. We reported on the ability hAECs to influence macrophage phenotype and chemotaxis. In this study, we aim elucidate contribution regulatory T (Tregs) polarisation downstream effects inflammation fibrosis a bleomycin model lung injury.Either CD45(+)/FoxP3(+) Tregs or CD45(+)/FoxP3 (-) non-Tregs were adoptively transferred into Rag1 (-/-) mice immediately prior...
Human amnion epithelial cells (hAECs) are nonimmunogenic, nontumorigenic, anti-inflammatory normally discarded with placental tissue. We reasoned that their profile of biological features, wide availability, and the lack ethical barriers to use could make these useful as a therapy in ischemic stroke.We tested efficacy acute (1.5 hours) or delayed (1-3 days) poststroke intravenous injection hAECs 4 established animal models cerebral ischemia. Animals included young (7-14 weeks) aged mice...
Mesenchymal stromal cells (MSCs) have significant therapeutic potential due to their ability differentiate into musculoskeletal lineages suitable for tissue-engineering, as well the immunomodulatory and pro-regenerative effects of paracrine factors that these secrete. Cues from extracellular environment, including physical stimuli such substrate stiffness, are strong drivers MSC differentiation, but upon activity not understood. This study, therefore sought determine impact stiffness on...
Since current treatments for both acute and chronic lung diseases are less than ideal, there has been recent interest in the use of cell-based therapies inflammatory disease. Specifically, human amnion epithelial cells (hAECs) have shown to reduce bleomycin-induced injury prevent subsequent loss respiratory function, primarily through modulation host immune response. The precise mechanisms this effect remain unclear. We aimed investigate potential hAECs mitigate surfactant protein C...