A5050866207- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Hepatitis B Virus Studies
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Infectious Diseases and Mycology
- Cell Image Analysis Techniques
- Hepatitis C virus research
- Bacterial biofilms and quorum sensing
- Immune cells in cancer
- Advanced Fluorescence Microscopy Techniques
- Mycotoxins in Agriculture and Food
- Chemokine receptors and signaling
- Platelet Disorders and Treatments
- Mitochondrial Function and Pathology
- Radiopharmaceutical Chemistry and Applications
- MicroRNA in disease regulation
- Bacteriophages and microbial interactions
- Orthopaedic implants and arthroplasty
University of Duisburg-Essen
2016-2025
Essen University Hospital
2012-2023
Imaging Center
2017
Otto-von-Guericke University Magdeburg
2009-2013
Helmholtz Centre for Infection Research
2007-2008
Aspergillus fumigatus is the most important airborne fungal pathogen causing life-threatening infections in immunocompromised patients. Macrophages and neutrophils are known to kill conidia, whereas hyphae killed mainly by neutrophils. Since too large be engulfed, possess an array of extracellular killing mechanisms including formation neutrophil traps (NETs) consisting nuclear DNA decorated with fungicidal proteins. However, until now NET response A. has only been demonstrated vitro,...
Significance Invasive pulmonary aspergillosis (IPA) is a frequently fatal lung disease of immunocompromised patients, and being increasingly reported in individuals with underlying respiratory diseases. Proven diagnosis IPA currently relies on biopsy detection diagnostic biomarkers serum, or bronchoalveolar lavage fluids. This study supports the use immunoPET/MR imaging for IPA, which so far not used diagnosis. The antibody-guided technique allows accurate, noninvasive rapid fungal infection...
Background and Purpose— Small extracellular vesicles (sEVs) obtained from mesenchymal stromal cells (MSCs) were shown to induce neurological recovery after focal cerebral ischemia in rodents reverse postischemic lymphopenia peripheral blood. Since blood cells, especially polymorphonuclear neutrophils (PMNs), contribute ischemic brain injury, we analyzed leukocyte responses sEVs investigated the role of PMNs sEV-induced neuroprotection. Methods— Male C57Bl6/j mice exposed transient...
The fungal pathogens Aspergillus fumigatus and Candida albicans are major health threats for immune-compromised patients. Normally, macrophages neutrophil granulocytes phagocytose inhaled conidia in the two-dimensional (2-D) environment of alveolar lumen or growing tissue microabscesses, which composed a three-dimensional (3-D) extracellular matrix. However, neither cellular dynamics, per-cell efficiency, outcome this interaction, nor environmental impact on process known. Live imaging shows...
Candida albicans strains that are homozygous at the mating type locus can spontaneously and reversibly switch from normal yeast morphology (white) to an elongated cell (opaque), which is mating-competent form of fungus. White-opaque switching also influences ability C. colonize proliferate in specific host niches its susceptibility defense mechanisms. We used live imaging observe interaction white opaque cells with phagocytic cells. For this purpose, we generated derivatives...
Abstract Invasive pulmonary aspergillosis (IPA) is a life-threatening lung disease of immunocompromised humans, caused by the opportunistic fungal pathogen Aspergillus fumigatus . Inadequacies in current diagnostic procedures mean that early diagnosis disease, critical to patient survival, remains major clinical challenge, and leading empiric use antifungal drugs emergence azole resistance. A non-invasive procedure allows both unambiguous detection IPA its response treatment therefore...
Invasive pulmonary aspergillosis (IPA) is a life-threatening lung disease of hematological malignancy or bone marrow transplant patients caused by the ubiquitous environmental fungus Aspergillus fumigatus. Current diagnostic tests for lack sensitivity as well specificity, and culture from invasive biopsy, considered gold standard IPA detection, slow often not possible in critically ill patients. In previous study, we reported development novel non-invasive procedure diagnosis based on...
Aspergillus fumigatus is a common airborne fungal pathogen for humans. It frequently causes an invasive aspergillosis (IA) in immunocompromised patients with poor prognosis. Potent antifungal drugs are very expensive and cause serious adverse effects. Their correct application requires early specific diagnosis of IA, which still not properly achievable. This work aims to detection A. by immunofluorescence the generation recombinant antibodies ELISA.The antigen Crf2 was isolated from human...
Polymorphonuclear neutrophils (PMN) mediate early immunity to infection but can also cause host damage if their effector functions are not controlled. Their lack or dysfunction is associated with severe health problems and thus the analysis of PMN physiology a central issue. One prerequisite for availability purified cells from primary organs. While human easily isolated peripheral blood, this approach less suitable mice due limited blood. Instead, bone marrow (BM) an available reservoir...
ABSTRACT Pneumococcal pili have been shown to influence pneumococcal colonization, disease development, and the inflammatory response in mice. The role of pilus-associated RrgA adhesin interactions with murine human macrophages was investigated. Expression enhanced uptake pneumococci by that abolished antibodies complement receptor 3 (CR3) not seen CR3-deficient macrophages. Recombinant RrgA, but pilus subunit RrgC, promoted CR3-mediated phagocytosis coated beads Flow cytometry showed...
Synapses are fundamental building blocks controlling and modulating the ‘behavior’ of brain networks. How their structural composition, most notably quantitative morphology underlie computational properties remains rather unclear, particularly in humans. Here, excitatory synaptic boutons (SBs) layer 4 (L4) temporal lobe neocortex (TLN) were quantitatively investigated. Biopsies from epilepsy surgery used for fine-scale tomographic electron microscopy (EM) to generate 3D-reconstructions SBs....
Dendritic cells (DCs) are crucial for the induction of potent antiviral immune responses. In contrast to immature DCs (iDCs), mature (mDCs) not permissive infection with herpes simplex virus type 1 (HSV-1). Here, we demonstrate that HSV-1 iDCs and mDCs induces autophagy, which promotes degradation lamin A/C, B1, B2 in only. This turn facilitates nuclear egress progeny viral capsids thus formation new infectious particles. contrast, protein levels remain stable HSV-1-infected due an...
Antidepressants have been reported to enhance stroke recovery independent of the presence depressive symptoms. They recently proposed exert their mood-stabilizing actions by inhibition acid sphingomyelinase (ASM), which catalyzes hydrolysis sphingomyelin ceramide. Their restorative action post-ischemia/reperfusion (I/R) still had be defined. Mice subjected middle cerebral artery occlusion or microvascular endothelial cells exposed oxygen-glucose deprivation were treated with vehicle...
Cell migration is the driving force behind dynamics of many diverse biological processes. Even though microscopy experiments are routinely performed today by which populations cells visualized in space and time, valuable information contained image data often disregarded because statistical analyses at level cell rather than single-cell level. Image-based systems biology a modern approach that aims quantitatively analyzing modeling processes developing novel strategies tools for...