A5036291953- Plant-Microbe Interactions and Immunity
- Fungal and yeast genetics research
- Plant Pathogens and Fungal Diseases
- Mycorrhizal Fungi and Plant Interactions
- Mycotoxins in Agriculture and Food
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Microbial Natural Products and Biosynthesis
- Wheat and Barley Genetics and Pathology
- Plant pathogens and resistance mechanisms
- Legume Nitrogen Fixing Symbiosis
- Bacterial Genetics and Biotechnology
- Lipid Membrane Structure and Behavior
- Microbial infections and disease research
- Slime Mold and Myxomycetes Research
- Nematode management and characterization studies
- Cellular transport and secretion
- Plant Disease Resistance and Genetics
- Bacterial biofilms and quorum sensing
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Polysaccharides and Plant Cell Walls
- Microbial Metabolism and Applications
- Fungal Infections and Studies
- Peptidase Inhibition and Analysis
University of East Anglia
2020-2025
Sainsbury Laboratory
2020-2025
Norwich Research Park
2020-2024
University of Exeter
2011-2019
Abstract Plants use pattern recognition receptors to defend themselves from microbial pathogens. These recognize pathogen-associated molecular patterns (PAMPs) and activate signaling pathways that lead immunity. In rice (Oryza sativa), the chitin elicitor binding protein (CEBiP) recognizes oligosaccharides released cell walls of fungal Here, we show blast fungus Magnaporthe oryzae overcomes this first line plant defense by secreting an effector protein, Secreted LysM Protein1 (Slp1), during...
To cause rice blast disease, the fungus Magnaporthe oryzae develops a pressurized dome-shaped cell called an appressorium, which physically ruptures leaf cuticle to gain entry plant tissue. Here, we report that toroidal F-actin network assembles in appressorium by means of four septin guanosine triphosphatases, polymerize into dynamic, hetero-oligomeric ring. Septins scaffold F-actin, via ezrin-radixin-moesin protein Tea1, and phosphatidylinositide interactions at plasma membrane. The ring...
Trichoderma hamatum strain GD12 is unique in that it can promote plant growth, activate biocontrol against pre- and post-emergence soil pathogens induce systemic resistance to foliar pathogens. This study extends previous work lettuce demonstrate confer beneficial agronomic traits other plants, providing examples of growth promotion the model dicot, Arabidopsis thaliana induced Magnaporthe oryzae monocot rice. We further characterize lettuce-T. interaction show bran extracts from an...
Magnaporthe oryzae is the causal agent of rice blast disease, most devastating disease cultivated (Oryza sativa) and a continuing threat to global food security. To cause fungus elaborates specialized infection cell called an appressorium, which breaches cuticle leaf, allowing entry plant tissue. Here, we show that exocyst complex localizes tips growing hyphae during vegetative growth, ahead Spitzenkörper, required for polarized exocytosis. However, infection-related development,...
Magnaporthe oryzae is the causal agent of rice blast disease, most widespread and serious disease cultivated rice. Live cell imaging quantitative 4D image analysis have provided new insight into mechanisms by which fungus infects host cells spreads rapidly in plant tissue. In this video review article, we apply live approaches to understanding developmental biology disease. To gain entry plants, M. develops a specialised infection structure called an appressorium, unicellular dome-shaped...
ABSTRACT Many plant pathogenic fungi have the capacity to infect their hosts using specialised cells called appressoria. These structures act as a gateway between fungus and host, allowing entry internal tissues. Appressoria apply enormous physical force rupture surface, or use battery of enzymes digest cuticle cell wall. also facilitate focal secretion effectors at point infection suppress immunity. develop in response characteristics leaf starvation stress signals from plant. Appressorium...
The rice blast fungus Magnaporthe oryzae uses a pressurized infection cell called an appressorium to drive rigid penetration peg through the leaf cuticle. vast internal pressure of is very challenging investigate, leaving our understanding cellular mechanics plant incomplete. Here, using fluorescence lifetime imaging membrane-targeting molecular mechanoprobe, we quantify changes in membrane tension M. oryzae. We show that extreme leads large-scale spatial heterogeneities mechanics, much...
Abstract The rice blast fungus Magnaporthe oryzae secretes a battery of effector proteins to facilitate host infection. Among these effectors, Pwl2 was first identified as specificity determinant for infection weeping lovegrass ( Eragrostis curvula ) and is also recognised by the barley Mla3 resistance gene. However, its biological activity not known. Here we show that PWL2 expression regulated Pmk1 MAP kinase during cell-to-cell movement M. at plasmodesmata (PD)-containing pit field sites....
Invasive pulmonary aspergillosis (IPA) is a life-threatening lung disease of hematological malignancy or bone marrow transplant patients caused by the ubiquitous environmental fungus Aspergillus fumigatus. Current diagnostic tests for lack sensitivity as well specificity, and culture from invasive biopsy, considered gold standard IPA detection, slow often not possible in critically ill patients. In previous study, we reported development novel non-invasive procedure diagnosis based on...
To cause rice blast disease, the filamentous fungus Magnaporthe oryzae secretes a battery of effector proteins into host plant tissue to facilitate infection. Effector-encoding genes are expressed only during infection and show very low expression other developmental stages. How gene is regulated in such precise manner invasive growth by M. not known. Here, we report forward-genetic screen identify regulators expression, based on selection mutants that constitutive expression. Using this...
Summary Glycosylphosphatidylinositol (GPI) anchoring plays key roles in many biological processes by targeting proteins to the cell wall; however, its are largely unknown plant pathogenic fungi. Here, we reveal of GPI Magnaporthe oryzae during infection. The GPI‐anchored were found highly accumulate appressoria and invasive hyphae. Disruption GPI7 , a anchor‐pathway gene, led significant reduction virulence. Δ gpi7 mutant showed defects penetration growth. This also displayed wall...
Trichoderma species are ubiquitous soil fungi that hold enormous potential for the development of credible alternatives to agrochemicals and synthetic fertilizers in sustainable crop production. In this paper, we show substantial improvements plant productivity can be met by genetic modification a plant-growth-promoting biocontrol strain hamatum, but these obtained absence disease pressure only. Using quantitative monoclonal antibody-based ELISA, an N-acetyl-β-d-glucosaminidase-deficient...
Abstract The pathogenic life cycle of the rice blast fungus Magnaporthe oryzae involves a series morphogenetic changes, essential for its ability to cause disease. smo mutation was identified > 25 years ago, and affects shape development diverse cell types in M. oryzae, including conidia, appressoria, asci. All attempts clone SMO1 gene by map-based cloning or complementation have failed over many years. Here, we report identification combination bulk segregant analysis comparative...
The dematiaceous (melanized) fungus Scedosporium prolificans is an emerging and frequently fatal pathogen of immunocompromised humans which, along with the closely related fungi Pseudallescheria boydii, apiospermum S. aurantiacum in Pseudallescheria-Scedosporium complex, a contributing aetiology to tsunami lung central nervous system infections near-drowning victims who have aspirated water laden spores. At present, natural habitat largely unknown, accurate detection methods are needed...
ABSTRACT Wheat, the most important food crop, is threatened by a blast disease pandemic. Here, we show that clonal lineage of wheat fungus recently spread to Asia and Africa following two independent introductions from South America. Through combination genome analyses laboratory experiments, decade-old pandemic can be controlled Rmg8 resistance gene sensitive strobilurin fungicides. However, also highlight potential clone evolve fungicide-insensitive variants sexually recombine with African...
The rice blast fungus Magnaporthe oryzae causes a devastating disease which threatens global production. In spite of intense study, the biology plant tissue invasion during remains poorly understood. Here we report high resolution, transcriptional profiling study entire plant-associated development fungus. Our analysis revealed major temporal changes in fungal gene expression infection. Pathogen could be classified into 10 modules temporally co-expressed genes, providing evidence induction...
SUMMARY Many of the world’s most devastating crop diseases are caused by fungal pathogens which elaborate specialized infection structures to invade plant tissue. Here we present a quantitative mass spectrometry-based phosphoproteomic analysis infection-related development rice blast fungus Magnaporthe oryzae , threatens global food security. We mapped 8,005 phosphosites on 2,062 proteins, revealing major re-wiring phosphorylation-based signaling cascades during infection. Comparing...
Abstract To cause rice blast disease the filamentous fungus Magnaporthe oryzae secretes a battery of effector proteins into host plant tissue to facilitate infection. Effector-encoding genes are expressed only during infection and show very low expression other developmental stages. How gene is regulated in such precise manner invasive growth by M. not known. Here, we report forward-genetic screen identify regulators expression, based on selection mutants that constitutive expression. Using...