A5022974974- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Fungal and yeast genetics research
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Transgenic Plants and Applications
- Toxin Mechanisms and Immunotoxins
- Genomics and Phylogenetic Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Stress Responses and Tolerance
- Polysaccharides and Plant Cell Walls
- Fungal Biology and Applications
- GABA and Rice Research
- Genetic Mapping and Diversity in Plants and Animals
- Plant Genetic and Mutation Studies
- Mycorrhizal Fungi and Plant Interactions
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Yeasts and Rust Fungi Studies
- Rice Cultivation and Yield Improvement
- Chromosomal and Genetic Variations
Yamagata University
2025
Iwate Biotechnology Research Center
2015-2024
Tokyo University of Agriculture
2017-2023
Kochi University of Technology
2017
Rice University
2012
Chiba Institute of Technology
2009
Max Planck Institute for Plant Breeding Research
2003-2005
University of Basel
2005
Sainsbury Laboratory
2005
John Innes Centre
2005
Abstract Plants use pattern recognition receptors to defend themselves from microbial pathogens. These recognize pathogen-associated molecular patterns (PAMPs) and activate signaling pathways that lead immunity. In rice (Oryza sativa), the chitin elicitor binding protein (CEBiP) recognizes oligosaccharides released cell walls of fungal Here, we show blast fungus Magnaporthe oryzae overcomes this first line plant defense by secreting an effector protein, Secreted LysM Protein1 (Slp1), during...
Abstract To subvert rice (Oryza sativa) host defenses, the devastating ascomycete fungus pathogen Magnaporthe oryzae produces a battery of effector molecules, including some with avirulence (AVR) activity, which are recognized by resistance (R) proteins resulting in rapid and effective activation innate immunity. isolate novel genes from M. oryzae, we examined DNA polymorphisms secreted protein predicted genome sequence 70-15 looked for an association AVR activity. This large-scale study...
To cause plant diseases, pathogenic micro-organisms secrete effector proteins into host tissue to suppress immunity and support pathogen growth. Bacterial pathogens have evolved several distinct secretion systems target proteins, but whether fungi, which the major diseases of most crop species, also require different secretory mechanisms is not known. Here we report that rice blast fungus Magnaporthe oryzae possesses two effectors during infection. Cytoplasmic effectors, are delivered cells,...
The Oryza sativa (rice) resistance gene Pia confers to the blast fungus Magnaporthe oryzae carrying AVR-Pia avirulence gene. To clone Pia, we employed a multifaceted genomics approach. First, selected 12 R-gene analog (RGA) genes encoding nucleotide binding site-leucine rich repeats (NBS-LRRs) proteins from region on chromosome 11 that shows linkage Pia. By using seven rice accessions, examined association between phenotypes and DNA polymorphisms in 10 genes, which revealed three...
Advances in genome sequencing technologies have enabled researchers and breeders to rapidly associate phenotypic variation sequence differences. We recently took advantage of next-generation technology develop MutMap, a method that allows rapid identification causal nucleotide changes rice mutants by whole resequencing pooled DNA mutant F2 progeny derived from crosses made between candidate the parental line. Here we describe MutMap+, versatile extension identifies mutations comparing SNP...
Plants have evolved intracellular immune receptors to detect pathogen proteins known as effectors. How these effectors remains poorly understood. Here we describe the structural basis for direct recognition of AVR-Pik, an effector from rice blast pathogen, by NLR receptor Pik. AVR-PikD binds a dimer Pikp-1 HMA integrated domain with nanomolar affinity. The crystal structure Pikp-HMA/AVR-PikD complex enabled design mutations alter protein interaction in yeast and vitro, perturb...
Attack and counter-attack impose strong reciprocal selection on pathogens hosts, leading to development of arms race evolutionary dynamics. Here we show that Magnaporthe oryzae avirulence gene AVR-Pik the cognate rice resistance (R) Pik are highly variable, with multiple alleles in which DNA replacements cause amino acid changes. There is tight recognition specificity by various alleles. We found physically binds N-terminal coiled-coil domain a yeast two-hybrid assay as well an planta...
Summary Next‐generation sequencing allows the identification of mutations responsible for mutant phenotypes by whole‐genome resequencing and alignment to a reference genome. However, when resequenced cultivar/line displays significant structural variation from genome, in genome regions missing (gaps) cannot be identified simple alignment. Here we report on method called ‘ M ut ap‐ G ap’, which involves delineating candidate region harboring mutation interest using recently reported ap...
Magnaporthe oryzae (anamorph Pyricularia oryzae) is the causal agent of blast disease Poaceae crops and their wild relatives. To understand genetic mechanisms that drive host specialization M. oryzae, we carried out whole genome resequencing four isolates from rice (Oryza sativa), one foxtail millet (Setaria italica), three S. viridis, isolate each finger (Eleusine coracana), wheat (Triticum aestivum) oat (Avena in addition to an a sister species grisea, infects grass Digitaria sanguinalis....
Plant pathogens have optimized their own effector sets to adapt hosts. However, certain effectors, regarded as core are conserved among various pathogens, and may therefore play an important common role in pathogen virulence. We report here that the widely distributed fungal NIS1 targets host immune components transmit signaling from pattern recognition receptors (PRRs) plants. two Colletotrichum spp. suppressed hypersensitive response oxidative burst, both of which induced by...
Plants are constantly exposed to environmental changes and need integrate multiple external stress cues. Calcium-dependent protein kinases (CDPKs) implicated as major primary Ca 2+ sensors in plants. CDPK activation, like activation of mitogen-activated (MAPKs), is triggered by biotic abiotic stresses, although distinct stimulus-specific responses induced. To investigate whether CDPKs part an underlying mechanism guarantee response specificity, we identified CDPK-controlled signaling...
The type III restriction endonuclease EcoP15I was used in isolating fragments of 26 bp from defined positions cDNAs. We call this substantially improved variant to the conventional serial analysis gene expression (SAGE) procedure “SuperSAGE.” By applying SuperSAGE Magnaporthe grisea (blast)-infected rice leaves, profiles both host and blast fungus were simultaneously monitored by making use fully sequenced genomes organisms, revealing that hydrophobin is most actively transcribed M....
SUMMARY Mitogen-activated protein kinases (MAPKs) play pivotal roles in the signal transduction pathway of plant defence responses against pathogens. A search for MAPK-interacting proteins revealed an interaction between a Nicotiana benthamiana MAPK, SIPK (NbSIPK) and cytosolic Hsp90 (NbHsp90c-1) yeast two-hybrid assay. To study function disease resistance, we silenced NbHsp90c-1 N. by virus-induced gene silencing (VIGS) with Potato virus X (PVX). plants exhibited: (1) stunted phenotype, (2)...
Summary Treatment of suspension‐cultured cells rice ( Oryza sativa L.) with cell wall extract blast fungus Magnaporthe grisea ) elicits a rapid generation H 2 O , alkalinization culture medium, and eventual death. To elucidate genes involved in these processes, we exploited SAGE (Serial Analysis Gene Expression) technique for the molecular analysis death treated elicitor. Among downregulated elicitor‐treated cells, BI‐1 gene coding Bax inhibitor was identified. Transgenic overexpressing...
To search for virulence effector genes of the rice blast fungus, Magnaporthe oryzae, we carried out a large-scale targeted disruption 78 putative secreted proteins that are expressed during early stages infection M. oryzae. Disruption majority did not affect growth, conidiation, or pathogenicity One exception was gene MC69. The mc69 mutant showed severe reduction in symptoms on and barley, indicating importance MC69 exhibit changes saprophytic growth conidiation. Microscopic analysis...
Summary Vesicle trafficking including the exocytosis pathway is intimately associated with host immunity against pathogens. However, we still have insufficient knowledge about how it contributes to immunity, and pathogen factors affect it. In this study, explore that interact Magnaporthe oryzae effector AVR ‐Pii. Gel filtration chromatography co‐immunoprecipitation assays identified a 150 kDa complex of proteins in soluble fraction comprising ‐Pii OsExo70‐F2 OsExo70‐F3, two rice Exo70...
Microbial plant pathogens secrete effector proteins, which manipulate the host to promote infection. Effectors can be recognized by intracellular nucleotide-binding leucine-rich repeat (NLR) receptors, initiating an immune response. The AVR-Pik from rice blast fungus Magnaporthe oryzae is a pair of NLR Pik-1 and Pik-2. contains noncanonical integrated heavy-metal-associated (HMA) domain, directly binds activate defenses. targets are also HMA-domain-containing namely isoprenylated proteins...
Throughout their evolution, plant nucleotide-binding leucine-rich-repeat receptors (NLRs) have acquired widely divergent unconventional integrated domains that enhance ability to detect pathogen effectors. However, the functional dynamics drive evolution of NLRs with (NLR-IDs) remain poorly understood. Here, we reconstructed evolutionary history an NLR locus prone domain integration and experimentally tested hypotheses about NLR-IDs. We show rice (Oryza sativa) Pias recognizes effector...
Studies focused solely on single organisms can fail to identify the networks underlying host-pathogen gene-for-gene interactions. Here, we integrate genetic analyses of rice (Oryza sativa, host) and blast fungus (Magnaporthe oryzae, pathogen) uncover a new pathogen recognition specificity nucleotide-binding domain leucine-rich repeat protein (NLR) immune receptor Pik, which mediates resistance M. oryzae expressing avirulence effector gene AVR-Pik. Rice Piks-1, encoded by an allele Pik-1,...
Abstract Plant nucleotide-binding leucine-rich repeat (NLRs) immune receptors directly or indirectly recognize pathogen-secreted effector molecules to initiate plant defense. Recognition of multiple pathogens by a single NLR is rare and usually occurs via monitoring for changes host proteins; few characterized NLRs have been shown effectors. The barley (Hordeum vulgare) gene Mildew locus (Mla) has undergone functional diversification, the proteins encoded different Mla alleles host-adapted...
A cellular signal transduction pathway induced by the polyamine, spermine (Spm), and transmitted mitochondrial dysfunction is proposed in tobacco. In this investigation, we further resolve identifying a subset of hypersensitive response (HR) marker genes as downstream components. previous report, identified harpin-induced 1 (HIN1) two closely related responsive to Spm. Other HR genes, HSR203J, HMGR, HSR201, HSR515, are also Spm-responsive. Induction these including HIN1, Spm was suppressed...