A5055388455- Dental materials and restorations
- Dental Implant Techniques and Outcomes
- Dental Erosion and Treatment
- Dental Research and COVID-19
- Photopolymerization techniques and applications
- Lipid Membrane Structure and Behavior
- Bone Tissue Engineering Materials
- Electrostatics and Colloid Interactions
- Additive Manufacturing and 3D Printing Technologies
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Orthopaedic implants and arthroplasty
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Microfluidic and Bio-sensing Technologies
- Cocoa and Sweet Potato Agronomy
- Advanced materials and composites
- Molecular Biology Techniques and Applications
- Orthodontics and Dentofacial Orthopedics
- Advances in Cucurbitaceae Research
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Polymer Surface Interaction Studies
- Analytical Chemistry and Sensors
- Corrosion Behavior and Inhibition
Nihon University
2015-2024
Shinshu University
2014-2024
Iwate Biotechnology Research Center
2007-2019
Ōtani University
2013-2018
Chugoku Electric Power (Japan)
2015-2016
University of Dental Medicine
2006-2011
Iwate University
2009
Yamaguchi University
2009
National Institute of Advanced Industrial Science and Technology
1997-2008
Japanese Red Cross Narita Hospital
2008
Abstract To subvert rice (Oryza sativa) host defenses, the devastating ascomycete fungus pathogen Magnaporthe oryzae produces a battery of effector molecules, including some with avirulence (AVR) activity, which are recognized by resistance (R) proteins resulting in rapid and effective activation innate immunity. isolate novel genes from M. oryzae, we examined DNA polymorphisms secreted protein predicted genome sequence 70-15 looked for an association AVR activity. This large-scale study...
The Oryza sativa (rice) resistance gene Pia confers to the blast fungus Magnaporthe oryzae carrying AVR-Pia avirulence gene. To clone Pia, we employed a multifaceted genomics approach. First, selected 12 R-gene analog (RGA) genes encoding nucleotide binding site-leucine rich repeats (NBS-LRRs) proteins from region on chromosome 11 that shows linkage Pia. By using seven rice accessions, examined association between phenotypes and DNA polymorphisms in 10 genes, which revealed three...
Advances in genome sequencing technologies have enabled researchers and breeders to rapidly associate phenotypic variation sequence differences. We recently took advantage of next-generation technology develop MutMap, a method that allows rapid identification causal nucleotide changes rice mutants by whole resequencing pooled DNA mutant F2 progeny derived from crosses made between candidate the parental line. Here we describe MutMap+, versatile extension identifies mutations comparing SNP...
Summary Salt tolerance quantitative trait loci analysis of rice has revealed that the SKC 1 locus, which is involved in a higher K + /Na ratio shoots, corresponds to Os HKT 1;5 gene encoding Na ‐selective transporter. However, physiological roles exposed salt stress remain elusive, and no disruption mutants have been characterized date. In this study, we dissected two independent T‐ DNA insertional mutants. Measurements ion contents tissues 22 tracer imaging experiments showed...
Summary Next‐generation sequencing allows the identification of mutations responsible for mutant phenotypes by whole‐genome resequencing and alignment to a reference genome. However, when resequenced cultivar/line displays significant structural variation from genome, in genome regions missing (gaps) cannot be identified simple alignment. Here we report on method called ‘ M ut ap‐ G ap’, which involves delineating candidate region harboring mutation interest using recently reported ap...
The type III restriction endonuclease EcoP15I was used in isolating fragments of 26 bp from defined positions cDNAs. We call this substantially improved variant to the conventional serial analysis gene expression (SAGE) procedure “SuperSAGE.” By applying SuperSAGE Magnaporthe grisea (blast)-infected rice leaves, profiles both host and blast fungus were simultaneously monitored by making use fully sequenced genomes organisms, revealing that hydrophobin is most actively transcribed M....
Drought is the major constraint to increase yield in chickpea (Cicer arietinum). Improving drought tolerance therefore of outmost importance for breeding. However, complexity trait allowed only marginal progress. A solution current stagnation expected from innovative molecular tools such as transcriptome analyses providing insight into stress-related gene activity, which combined with markers and expression (e)QTL mapping, may accelerate knowledge-based SuperSAGE, an improved version serial...
Summary Treatment of suspension‐cultured cells rice ( Oryza sativa L.) with cell wall extract blast fungus Magnaporthe grisea ) elicits a rapid generation H 2 O , alkalinization culture medium, and eventual death. To elucidate genes involved in these processes, we exploited SAGE (Serial Analysis Gene Expression) technique for the molecular analysis death treated elicitor. Among downregulated elicitor‐treated cells, BI‐1 gene coding Bax inhibitor was identified. Transgenic overexpressing...
Abstract Expression of nuclear-encoded plastid proteins and import those into plastids are indispensable for biogenesis. One possible cellular mechanism that coordinates these two essential processes is retrograde signaling from to the nucleus. However, molecular details how this occurs remain elusive. Using protein import2 mutant Arabidopsis (Arabidopsis thaliana), which lacks atToc159 receptor, we demonstrate expression photosynthesis-related nuclear genes tightly coordinated with their...
To search for virulence effector genes of the rice blast fungus, Magnaporthe oryzae, we carried out a large-scale targeted disruption 78 putative secreted proteins that are expressed during early stages infection M. oryzae. Disruption majority did not affect growth, conidiation, or pathogenicity One exception was gene MC69. The mc69 mutant showed severe reduction in symptoms on and barley, indicating importance MC69 exhibit changes saprophytic growth conidiation. Microscopic analysis...
Root and tuber crops are a major food source in tropical Africa. Among these several species the monocotyledonous genus Dioscorea collectively known as yam, staple crop that contributes enormously to subsistence socio-cultural lives of millions people, principally West Central Yam cultivation is constrained by factors, yam can be considered neglected "orphan" would benefit from improvement efforts. However, lack genetic genomic tools has impeded this crop. To accelerate marker-assisted...
Bitter gourd (Momordica charantia) is an important vegetable and medicinal plant in tropical subtropical regions globally. In this study, the draft genome sequence of a monoecious bitter inbred line, OHB3-1, was analyzed. Through Illumina sequencing de novo assembly, scaffolds 285.5 Mb length were generated, corresponding to ∼84% estimated size (339 Mb). sequence, 45,859 protein-coding gene loci identified, transposable elements accounted for 15.3% whole genome. According synteny mapping...
Serial analysis of gene expression (SAGE) was applied for profiling expressed genes in rice seedlings. In the SAGE method, a 9-11 bp fragment (tag) represents each transcript, and frequency tag sample directly reflects abundance respective mRNA. We studied 10 122 tags derived from 5921 (Oryza sativa L.) seedlings, among which only 1367 (23.1%) matched cDNA or EST sequences DNA database. showed that most highly seedlings belong to category housekeeping (genes encoding ribosomal proteins...
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High-Throughput (HT-) SuperSAGE". is method digital gene expression profiling that allows isolation 26-bp tag fragments from expressed transcripts. In present protocol, index (barcode) sequences are employed to discriminate tags different samples. Such barcodes allow researchers analyze transcriptomes many samples in single sequencing run by simply pooling libraries. Here, we demonstrated...
To date, there is no evidence that internal anti-rotation configurations are better than external ones. As part of a study to clarify the features and advantages configurations, objective present investigation was compare evaluate marginal fit size microgap at implant-abutment interface for several configurations. this end, three connection two hex assemblies were examined in study. The (I-A interface) evaluated using geometrical factors: vertical horizontal discrepancies microgap. Marginal...