The gene regulatory information is hardwired in the promoter regions formed by cis-regulatory elements that bind specific transcription factors (TFs). Hence, establishing architecture of plant promoters fundamental to understanding expression. determination circuits controlled each TF and identification sequences for all genes have been identified as two goals Multinational Coordinated Arabidopsis thaliana Functional Genomics Project Steering Committee (June 2002).AGRIS an resource...

Drought is the major constraint to increase yield in chickpea (Cicer arietinum). Improving drought tolerance therefore of outmost importance for breeding. However, complexity trait allowed only marginal progress. A solution current stagnation expected from innovative molecular tools such as transcriptome analyses providing insight into stress-related gene activity, which combined with markers and expression (e)QTL mapping, may accelerate knowledge-based SuperSAGE, an improved version serial...

Abstract Background Core promoters are the gene regulatory regions most proximal to transcription start site (TSS), central formation of pre-initiation complexes and for combinatorial regulation. The DNA elements required core promoter function in plants poorly understood. To establish sequence motifs that characterize plant compare them corresponding sequences animals, we took advantage available full-length cDNAs (FL-cDNAs) predicted upstream carry out analysis 12,749 Arabidopsis...

The combination of high-throughput transcript profiling and next-generation sequencing technologies is a prerequisite for genome-wide comprehensive transcriptome analysis. Our recent innovation deepSuperSAGE based on an advanced SuperSAGE protocol its with massively parallel pyrosequencing Roche's 454 platform. As demonstration the power this combination, we have chosen salt stress transcriptomes roots nodules third most important legume crop chickpea (Cicer arietinum L.). While our report...

Rapeseed (Brassica napus L.) is grown in different geographical regions of the world. It adapted to environments by modification flowering time and requirement for cold. A broad variation exists from very early-flowering spring-type late-flowering winter cultivars which only flower after exposure an extended cold period. B. allopolyploid species resulted hybridization between rapa oleracea. In Arabidopsis thaliana, PEBP-domain genes FLOWERING LOCUS-T (FT) TERMINAL FLOWER-1 (TFL1) are...

Rapeseed (Brassica napus L.), one of the most important sources vegetable oil and protein-rich meals worldwide, is adapted to different geographical regions by modification flowering time. cultivars have day length vernalization requirements, which categorize them into winter, spring, semiwinter ecotypes. To gain a deeper insight genetic factors controlling floral transition in B. napus, we performed RNA sequencing (RNA-seq) doubled haploid line, Ningyou7, at developmental stages temperature...

A survey of entomopathogenic nematodes (EPN) was conducted in Morocco during 2015 and 2016 three regions with varying topography, climate vegetation (Meknès-El Hajeb, Ifrane Tafilalet). EPN were isolated from 14 169 soil samples (8%). Molecular morphological techniques used for species determination. Nine contained heterorhabditids five steinernematids. Eight identified as Heterorhabditis bacteriophora Poinar 1976, two steinernematids Steinernema feltiae (Filipjev, 1934), belonging to the S....

Abstract Entomopathogenic nematodes are effective biocontrol agents against arthropod pests. However, their efficacy is limited due to sensitivity environmental extremes. The objective of the present study was establish a foundation genetic-based selection tools for beneficial traits heat and desiccation tolerance in entomopathogenic nematodes. Screening natural populations enabled us create diverse genetic phenotypic pool. Gene expression patterns genomic variation were studied isolates....

Symbiotic nitrogen fixation (SNF) in root nodules of grain legumes such as chickpea is a highly complex process that drastically affects the gene expression patterns both prokaryotic well eukaryotic interacting cells. A successfully established symbiotic relationship requires mutual signaling mechanisms and continuous adaptation metabolism involved cells to varying environmental conditions. Although some these processes are understood today many molecular underlying SNF, especially chickpea,...

Plants are sessile organisms subjected to many environmental adversities. For their survival they must sense and respond biotic abiotic stresses efficiently. During this process, protein kinases essential in the perception of stimuli, triggering signaling cascades. Kinases among largest most important gene families for biotechnological purposes, bringing challenges bioinformaticians due combination conserved domains besides diversified regions. Cowpea [Vigna unguiculata (L.) Walp.] is an...

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Summary The entomopathogenic nematode (EPN), Heterorhabditis bacteriophora , is an important biological control agent worldwide. Industrially produced EPN need to meet the climatic requirements for of pests in field agriculture autumn and spring when temperatures are low. For this trait (virulence at low temperature), previous improvement attempts relied on phenotypic selection selected had stability. use molecular markers can increase efficacy breeding by tracking traits associated with...

The commercial use of the entomopathogenic nematode Heterorhabditis bacteriophora as a biocontrol agent against noxious insects is limited due to its relatively short shelf-life. Longevity dauer juveniles (DJ) during storage and in transit end users considerably restricted by environmental stresses. As derivative stress triggered factors, oxidative causes strong internal metabolic imbalance leading lifespan reduction. In this study, relation between DJ tolerance longevity H. was investigated...

Abstract In addition to the importance of many Dioscorea species (yams) as starchy staple food, some representatives are known and still used a source for steroidal sapogenin diosgenin, which, besides phytosterols derived from tall-oil, is an important precursor partial synthesis steroids pharmaceutical research applications. While in edible yams diosgenin content should be low possible, high yield compound preferable cultivars which grown extraction sterols. past, miscalculations...

Abstract Yellow Sigatoka caused by the ascomycete Mycosphaerella musicola Leach, is one of most severe banana diseases worldwide, which spread in growing areas, until Black Sigatoka, a more aggressive disease fijiensis , appeared. Because highly devastating nature latter pathogen, recent research almost exclusively focused on M. fijiensis. To close gap knowledge and to study population structure Sigatoka‐infested areas we cloned characterized versatile set 26 polymorphic locus‐specific...

Summary The entomopathogenic nematode (EPN), Heterorhabditis bacteriophora , is a biocontrol agent with almost world-wide distribution, thus providing huge source of biodiversity available for exploitation in breeding programmes to improve beneficial traits. For management economically important insect pests, the mass-produced liquid culture, formulated inert carriers, and transported under controlled temperature conditions maintain its quality. potential materials defined by their genetic...