A5028737517- Nitrogen and Sulfur Effects on Brassica
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- Lipid metabolism and biosynthesis
- Plant Stress Responses and Tolerance
- CRISPR and Genetic Engineering
- Plant nutrient uptake and metabolism
- Genetic Mapping and Diversity in Plants and Animals
- Genetic diversity and population structure
- Soybean genetics and cultivation
- Plant tissue culture and regeneration
- Genomics, phytochemicals, and oxidative stress
- Light effects on plants
- Plant Pathogens and Resistance
- Plant Gene Expression Analysis
Senckenberg Research Institute and Natural History Museum Frankfurt/M
2020-2023
Justus-Liebig-Universität Gießen
2014-2023
Homoeologous exchanges (HEs) have been shown to generate novel gene combinations and phenotypes in a range of polyploid species. Gene presence/absence variation (PAV) is also major contributor genetic diversity. In this study, we show that there an association between these two events, particularly recent Brassica napus synthetic accessions, represent source diversity, which can be captured for the improvement important crop By assembling pangenome B. napus, 38% genes display PAV behaviour,...
Abstract Despite early domestication around 3000 BC, the evolutionary history of ancient allotetraploid species Brassica juncea (L.) Czern & Coss remains uncertain. Here, we report a chromosome-scale de novo assembly yellow-seeded B. genome by integrating long-read and short-read sequencing, optical mapping Hi-C technologies. Nuclear organelle phylogenies 480 accessions worldwide supported that is most likely single origin in West Asia, 8,000–14,000 years ago, via natural interspecific...
Drought stress has a negative effect on both seed yield and quality in Brassica napus (oilseed rape, canola). Here we show that while drought impairs the maternal plant performance, it also increases vigour of progeny stressed plants. We investigated transgenerational influence abiotic by detailed analysis yield, quality, seedling performance growth-related metabolic level. Seeds eight diverse winter oilseed rape genotypes were generated under well-watered conditions controlled-environment...
Genomic rearrangements arising during polyploidization are an important source of genetic and phenotypic variation in the recent allopolyploid crop Brassica napus. Exchanges among homoeologous chromosomes, due to interhomoeologue pairing, deletions without compensating duplications observed both natural B. napus synthetic Rearrangements large or small chromosome segments induce gene copy number (CNV) can potentially cause changes. Unfortunately, complex genome restructuring is difficult deal...
Flowering, the transition from vegetative to generative phase, is a decisive time point in lifecycle of plant. Flowering controlled by complex network transcription factors, photoreceptors, enzymes and miRNAs. In recent years, several studies gave rise hypothesis that this also strongly involved regulation other important processes ranging germination seed development through fundamental developmental yield-related traits. allopolyploid crop species Brassica napus, (genome AACC),...
Many of our major crop species are polyploids, containing more than one genome or set chromosomes. Polyploid crops present unique challenges, including difficulties in assembly, discriminating between multiple gene and sequence copies, genetic mapping, hindering use genomic data for genetics breeding. genomes may also be prone to structural variation, such as loss copies sequences (presence–absence variation) the presence genes (copy-number variation). Although two main types variation...
Flowering time, plant height and seed yield are strongly influenced by climatic day-length adaptation in crop plants. To investigate these traits under highly diverse field conditions the important oilseed Brassica napus, we performed a genome-wide association study using data from agroecological environments spanning three continents. A total of 158 European winter-type B.napus inbred lines were genotyped with 21,623 unique, single-locus single-nucleotide polymorphism (SNP) markers 60 K-SNP...
Abstract Genetic models for polyploid crop adaptation provide important information relevant future breeding prospects. A well-suited model is Brassica napus , a recent allopolyploid closely related to Arabidopsis thaliana . Flowering time major trait determining life cycle synchronization with the environment. Here we unravel natural genetic variation in B. flowering regulators and investigate associations evolutionary diversification into different morphotypes. Deep sequencing of 35 was...
Summary Genome structural variation (SV) contributes strongly to trait in eukaryotic species and may have an even higher functional significance than single‐nucleotide polymorphism (SNP). In recent years, there been a number of studies associating large chromosomal scale SV ranging from hundreds kilobases all the way up few megabases key agronomic traits plant genomes. However, little or no efforts towards cataloguing small‐ (30–10 000 bp) mid‐scale (10 000–30 their impact on evolution...
Synthetic allohexaploid Brassica hybrids (2n = AABBCC) do not exist naturally, but can be synthesized by crosses between diploid and/or allotetraploid species. Using these hybrids, we aimed to identify how novel allohexaploids restore fertility and normal meiosis after formation. Chromosome inheritance, genome structure, meiotic behaviour were assessed in three segregating populations derived from the cross (B. napus × B. carinata) juncea using a combination of molecular marker genotyping,...
Rapeseed (Brassica napus L.), one of the most important sources vegetable oil and protein-rich meals worldwide, is adapted to different geographical regions by modification flowering time. cultivars have day length vernalization requirements, which categorize them into winter, spring, semiwinter ecotypes. To gain a deeper insight genetic factors controlling floral transition in B. napus, we performed RNA sequencing (RNA-seq) doubled haploid line, Ningyou7, at developmental stages temperature...
Abstract Plants in temperate areas evolved vernalisation requirement to avoid pre-winter flowering. In Brassicaceae , a period of extended cold reduces the expression flowering inhibitor FLOWERING LOCUS C ( FLC ) and paves way for downstream regulators. As with all polyploid species model allotetraploid Brassica napus (rapeseed, canola) is highly duplicated carries 9 annotated copies Bna.FLC . To investigate whether these multiple homeologs paralogs have retained their original function or...
Flowering time genes have a strong influence on successful reproduction and life cycle adaptation. However, their regulation is highly complex only well understood in diploid model systems. For crops with polyploid background from the genus Brassica, data flowering gene variation are scarce, although indispensable for modern breeding techniques like marker-assisted breeding. We deep-sequenced all paralogs of 35 Arabidopsis thaliana regulators using Sequence Capture followed by Illumina...
Gene copy number variation (CNV) is increasingly implicated in control of complex trait networks, particularly polyploid plants like rapeseed (Brassica napus L.) with an evolutionary history genome restructuring. Here we performed sequence capture to assay nucleotide and CNV a panel central flowering time regulatory genes across species-wide diversity set 280 B. accessions. The were chosen based on prior knowledge from Arabidopsis thaliana related Brassica species. Target enrichment was...
Abstract Key message A novel structural variant was discovered in the FLOWERING LOCUS T orthologue BnaFT.A02 by long-read sequencing. Nested association mapping an elite winter oilseed rape population revealed that this 288 bp deletion associates with early flowering, putatively modification of binding-sites for important flowering regulation genes. Perfect timing is crucial optimal pollination and high seed yield. Extensive previous studies behavior Brassica napus (canola, rapeseed)...
Spring droughts are expected to become more frequent in Central Europe as a result of climate change. Their coincidence with flowering biennial crops like winter oilseed rape (Brassica napus) can cause major impact for yield development. However, no data is available on the diversity genetic regulation drought tolerance during this stage under realistic conditions. Here, we assessed phenotypic plasticity response eight diverse B. napus accessions field-like conditions and linked their stress...
Roots play an immediate role as the interface for water acquisition. To improve sustainability in low-water environments, breeders of major crops must therefore pay closer attention to advantageous root phenotypes; however, complexity architecture response stress can be difficult quantify. Here, Sholl method, established technique from neurobiology used characterization neural network anatomy, was adapted more adequately describe responses osmotic stress. This method investigate influence...
Established allopolyploids are known to be genomically stable and fertile. However, in contrast, most newly resynthesized infertile meiotically unstable. Identifying the genetic factors responsible for genome stability formed allopolyploid is key understanding how 2 genomes come together form a species. One hypothesis that established may have inherited specific alleles from their diploid progenitors which conferred meiotic stability. Resynthesized Brassica napus lines often unstable...
Correct timing of flowering is critical for plants to produce enough viable offspring. In Arabidopsis thaliana (Arabidopsis), time regulated by an intricate network molecular signaling pathways. srr1-1 mutants lacking SENSITIVITY TO RED LIGHT REDUCED 1 (SRR1) expression flower early, particularly under short day (SD) conditions (1). SRR1 ensures that do not prematurely in such non-inductive controlling repression the key florigen FT. Here, we have examined role closely related crop species...