A5027164927- Plant-Microbe Interactions and Immunity
- Fungal and yeast genetics research
- Plant Pathogens and Fungal Diseases
- Mycorrhizal Fungi and Plant Interactions
- Mycotoxins in Agriculture and Food
- Plant Gene Expression Analysis
- Endoplasmic Reticulum Stress and Disease
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- Autophagy in Disease and Therapy
- Photosynthetic Processes and Mechanisms
- Microbial Natural Products and Biosynthesis
- Plant and Biological Electrophysiology Studies
- Lipid Membrane Structure and Behavior
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
Sainsbury Laboratory
2021-2025
University of East Anglia
2021-2025
Norwich Research Park
2021-2025
University of Göttingen
2019
Abstract The rice blast fungus Magnaporthe oryzae causes a devastating disease that threatens global (Oryza sativa) production. Despite intense study, the biology of plant tissue invasion during remains poorly understood. Here we report high-resolution transcriptional profiling study entire plant-associated development fungus. Our analysis revealed major temporal changes in fungal gene expression infection. Pathogen could be classified into 10 modules temporally co-expressed genes, providing...
Magnaporthe oryzae is the causal agent of rice blast disease, most widespread and serious disease cultivated rice. Live cell imaging quantitative 4D image analysis have provided new insight into mechanisms by which fungus infects host cells spreads rapidly in plant tissue. In this video review article, we apply live approaches to understanding developmental biology disease. To gain entry plants, M. develops a specialised infection structure called an appressorium, unicellular dome-shaped...
ABSTRACT Many plant pathogenic fungi have the capacity to infect their hosts using specialised cells called appressoria. These structures act as a gateway between fungus and host, allowing entry internal tissues. Appressoria apply enormous physical force rupture surface, or use battery of enzymes digest cuticle cell wall. also facilitate focal secretion effectors at point infection suppress immunity. develop in response characteristics leaf starvation stress signals from plant. Appressorium...
Many of the world's most devastating crop diseases are caused by fungal pathogens that elaborate specialized infection structures to invade plant tissue. Here, we present a quantitative mass-spectrometry-based phosphoproteomic analysis infection-related development rice blast fungus Magnaporthe oryzae, which threatens global food security. We mapped 8,005 phosphosites on 2,062 proteins following germination hydrophobic surface, revealing major re-wiring phosphorylation-based signaling...
The rice blast fungus Magnaporthe oryzae uses a pressurized infection cell called an appressorium to drive rigid penetration peg through the leaf cuticle. vast internal pressure of is very challenging investigate, leaving our understanding cellular mechanics plant incomplete. Here, using fluorescence lifetime imaging membrane-targeting molecular mechanoprobe, we quantify changes in membrane tension M. oryzae. We show that extreme leads large-scale spatial heterogeneities mechanics, much...
Abstract The rice blast fungus Magnaporthe oryzae secretes a battery of effector proteins to facilitate host infection. Among these effectors, Pwl2 was first identified as specificity determinant for infection weeping lovegrass ( Eragrostis curvula ) and is also recognised by the barley Mla3 resistance gene. However, its biological activity not known. Here we show that PWL2 expression regulated Pmk1 MAP kinase during cell-to-cell movement M. at plasmodesmata (PD)-containing pit field sites....
Abstract The rice blast fungus Magnaporthe oryzae secretes a battery of effector proteins to facilitate host infection. Among these effectors, Pathogenicity toward Weeping Lovegrass 2 (Pwl2) was originally identified as specificity determinant for the infection weeping lovegrass (Eragrostis curvula) and is also recognized by barley (Hordeum vulgare) Mla3 resistance protein. However, biological activity Pwl2 remains unknown. Here, we showed that Pmk1 MAP kinase regulates PWL2 expression...
Verticillium longisporum is a soilborne vascular fungal pathogen that has spread throughout the European oilseed rape cultivation area since 1980s and was detected in canola fields Canada 2014. In series of greenhouse field inoculation experiments using V. longisporum-resistant susceptible cultivars winter spring types rape, present study investigated potential dissemination by seeds Brassica napus. Greenhouse studies with DsRed-labeled isolate confirmed systemic growth from roots to seeds....
Oilseed rape, an important source of vegetable plant oil, is threatened by Verticillium longisporum, a soil-borne vascular fungal pathogen so far occurring in oilseed rape growing regions Europe and Canada. Despite intensive research into V. longisporum the last decades controlled conditions, basic knowledge still lacking about time course infection, temporal pattern colonization, disease development on field-grown plants. In this study, colonization roots, stem bases, stems with was...
The rice blast fungus Magnaporthe oryzae causes a devastating disease which threatens global production. In spite of intense study, the biology plant tissue invasion during remains poorly understood. Here we report high resolution, transcriptional profiling study entire plant-associated development fungus. Our analysis revealed major temporal changes in fungal gene expression infection. Pathogen could be classified into 10 modules temporally co-expressed genes, providing evidence induction...
SUMMARY Many of the world’s most devastating crop diseases are caused by fungal pathogens which elaborate specialized infection structures to invade plant tissue. Here we present a quantitative mass spectrometry-based phosphoproteomic analysis infection-related development rice blast fungus Magnaporthe oryzae , threatens global food security. We mapped 8,005 phosphosites on 2,062 proteins, revealing major re-wiring phosphorylation-based signaling cascades during infection. Comparing...
Abstract Many plant pathogenic fungi forcibly enter their hosts to cause disease. The rice blast fungus Magnaporthe oryzae , for example, infects plants using a specialised infection cell called an appressorium, which generates enormous turgor drive rigid penetration peg through the leaf cuticle. While these vast internal pressures are critical weapon in fungal host penetration, they have remained very challenging probe directly during invasion, leaving our understanding of extreme cellular...