A5014791123- Gut microbiota and health
- Polysaccharides and Plant Cell Walls
- Extracellular vesicles in disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Bacterial Infections and Vaccines
- Alzheimer's disease research and treatments
- Probiotics and Fermented Foods
- Fungal Biology and Applications
- Pharmacological Effects of Natural Compounds
- Polysaccharides Composition and Applications
- Immune Response and Inflammation
- Tryptophan and brain disorders
- RNA Interference and Gene Delivery
- Parkinson's Disease Mechanisms and Treatments
- Plant Stress Responses and Tolerance
- Plant biochemistry and biosynthesis
- Heavy Metals in Plants
- Calcium signaling and nucleotide metabolism
- Immune cells in cancer
- Microbial Metabolites in Food Biotechnology
- RNA regulation and disease
- Lysosomal Storage Disorders Research
- Lipid metabolism and biosynthesis
- Amoebic Infections and Treatments
- Barrier Structure and Function Studies
VIB-UGent Center for Inflammation Research
2021-2025
Ghent University
2021-2025
Nanchang University
2006-2025
State Key Laboratory of Food Science and Technology
2015-2025
Ghent University Hospital
2023-2025
Agricultural Genomics Institute at Shenzhen
2023-2024
Zhengzhou University
2024
Chinese Academy of Agricultural Sciences
2023-2024
Henan University
2023-2024
KU Leuven
2023
The ability of immune-modulating biologics to prevent and reverse pathology has transformed recent clinical practice. Full utility in the neuroinflammation space, however, requires identification both effective targets for local immune modulation a delivery system capable crossing blood-brain barrier. characterization small population regulatory T (Treg) cells resident brain presents one such potential therapeutic target. Here, we identified interleukin 2 (IL-2) levels as limiting factor...
Abstract The gut microbiota represents a diverse and dynamic population of microorganisms that can influence the health host. Increasing evidence supports role as key player in pathogenesis neurodegenerative diseases, including Alzheimer's disease (AD). Unfortunately, mechanisms behind interplay between pathogens AD are still elusive. It is known bacteria‐derived outer membrane vesicles (OMVs) act natural carriers virulence factors central players bacteria. Helicobacter pylori ( H. ) common...
Abstract Accumulating evidence indicates that gut microbiota dysbiosis is associated with increased blood–brain barrier (BBB) permeability and contributes to Alzheimer's disease (AD) pathogenesis. In contrast, the influence of on blood‐cerebrospinal fluid (CSF) has not yet been studied. Here, we report mice lacking display blood‐CSF disorganized tight junctions (TJs), which can be rescued by recolonization or supplementation short‐chain fatty acids (SCFAs). Our data reveal important only for...
Abstract Intracellular delivery of protein and RNA therapeutics represents a major challenge. Here, we develop highly potent engineered extracellular vesicles (EVs) by incorporating bio-inspired attributes required for effective delivery. These comprise an mini-intein with self-cleavage activity active cargo loading release, fusogenic VSV-G endosomal escape. Combining these components allows high efficiency recombination genome editing in vitro following EV-mediated Cre recombinase...
The effects of Lactobacillus plantarum (L. plantarum) NCU116 isolated from pickled vegetables on intestine mucosal immunity in cyclophosphamide treated mice were investigated. Animals divided into six groups: normal group (NIM), immunosuppression (IM), plus L. groups with three different doses (NCU-H, NCU-M, and NCU-L), Bifidobacterium BB12 as positive control (BB12). Results showed that the thymus indexes four treatment significantly higher than IM (2.02 ± 0.16) (p < 0.05) close to index...
Abstract Alzheimer’s disease (AD) is a chronic neurodegenerative characterized by the accumulation of amyloid β (Aβ) and neurofibrillary tangles. The last decade, it became increasingly clear that neuroinflammation plays key role in both initiation progression AD. Moreover, also presence peripheral inflammation has been extensively documented. However, still ambiguous whether this observed cause or consequence AD pathogenesis. Recently, studied using precursor protein (APP) overexpression...
Parkinson’s disease (PD) is the second most common neurodegenerative characterized by progressive degeneration of dopaminergic neurons in substantia nigra (SN). The main bioactive component green tea polyphenols (-)-epigallocatechin-3-gallate (EGCG) exerts protective effects against diseases such as and cancer. Therefore, this study investigated effect EGCG on amelioration neural damage a chronic PD mouse model induced α-synuclein preformed fibrils (α-syn-PFFs). A total 20 C57BL/6J female...
The balance of T helper cells 17 (Th17)/regulatory (Treg) plays a key role in maintaining normal immune response. It is well-known that cyclophosphamide (CTX) applied at high dose often damages the system by inhibiting cell proliferation. In this study, immunomodulating effects Lactobacillus plantarum NCU116 CTX-induced immunosuppression mice were investigated. Results showed levels cytokines interleukin (IL)-17 and IL-21 significantly increased after 10 days treatment with (46.92 ± 4.28...
<italic>Lactobacillus plantarum</italic>NCU116 promotes intestinal health by improving mucosal structure, metabolism and microbiota.
Extracellular vesicles (EVs) are promising tools to transfer macromolecular therapeutic molecules recipient cells, however, efficient functional intracellular protein delivery by EVs remains challenging. Here, we have developed novel and versatile systems that leverage selected molecular engineer for robust cytosolic both in vitro vivo. These systems, termed VSV-G plus EV-sorting Domain-Intein-Cargo (VEDIC) VSV-G-Foldon-Intein-Cargo (VFIC), exploit an engineered mini-intein (intein) with...