A5049046169- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Immune cells in cancer
- Tissue Engineering and Regenerative Medicine
- Hepatitis B Virus Studies
- Circular RNAs in diseases
- Hepatitis C virus research
- Pluripotent Stem Cells Research
- Blood groups and transfusion
- Adenosine and Purinergic Signaling
- HIV Research and Treatment
- Periodontal Regeneration and Treatments
- HIV, Drug Use, Sexual Risk
- HIV/AIDS drug development and treatment
- Epigenetics and DNA Methylation
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA modifications and cancer
- Cytomegalovirus and herpesvirus research
- Parvovirus B19 Infection Studies
- Cancer-related gene regulation
- Biosimilars and Bioanalytical Methods
- Viral Infections and Vectors
- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
University of Duisburg-Essen
2013-2024
Essen University Hospital
2015-2022
Ruhr University Bochum
2016
Institute of Virology of the Slovak Academy of Sciences
2015
Abstract Obtained from the right cell-type, mesenchymal stromal cell (MSC)-derived small extracellular vesicles (sEVs) promote stroke recovery. Within this process, microvascular remodeling plays a central role. Herein, we evaluated effects of MSC-sEVs on proliferation, migration, and tube formation human cerebral endothelial cells (hCMEC/D3) in vitro post-ischemic angiogenesis, brain neurological recovery after middle artery occlusion (MCAO) mice. In vitro, sEVs obtained hypoxic (1% O 2 ),...
The idea of collecting blood on a paper card and subsequently using the dried spots (DBS) for diagnostic purposes originated century ago. Since then, DBS testing decades has remained predominantly focused diagnosis infectious diseases especially in resource-limited settings or systematic screening newborns inherited metabolic disorders only recently have variety new innovative applications begun to emerge. For many years, pre-analytical variables were inappropriately considered field even...
Nowadays, dried blood spots (DBS) are primarily used to obtain diagnostic access risk collectives such as intravenous drug users, who prone infections with hepatitis B virus (HBV), C (HCV), and human immunodeficiency (HIV). Before DBS analyses can be in this context, however, a comprehensive evaluation of its performance characteristics must conducted. To the best our knowledge, current study presents for first time essential data Abbott ARCHITECT system, which is currently worldwide leading...
Abstract Mesenchymal stem/stromal cells (MSCs) provide therapeutic effects in many diseases. Contrary to initial hypotheses, they act a paracrine rather than cellular manner. To this end, extracellular vesicles (EVs) have been found mediate the effects, even when harvested from MSC‐conditioned cell culture supernatants. Lacking self‐replicating activity and being so small that MSC‐EV preparations can be sterilized by filtration, EVs several advantages as agents over therapeutics. At present,...
Small extracellular vesicles (sEVs) obtained from mesenchymal stromal cells (MSCs) were shown to induce ischemic neuroprotection in mice by modulating the brain infiltration of leukocytes and, specifically polymorphonuclear neutrophils. So far, effects MSC-sEVs only studied young rodents. We herein examined aged mice.
Mesenchymal stromal cells (MSCs) exert immunomodulatory effects, primarily through released extracellular vesicles (EVs). For the clinical grade manufacturing of MSC-EV products culture conditions need to support MSC expansion and allow potent products. Traditionally, MSCs are expanded in fetal bovine serum supplemented media. However, according good practice (GMP) guidelines use animal sera should be avoided. To this end, human platelet lysate (hPL) has been qualified as replacement....
The idea of collecting blood on a paper card and subsequently using the dried spots (DBS) for diagnostic purposes originated century ago. Since then, DBS testing decades has remained predominantly focused diagnosis infectious diseases especially in resource-limited settings or systematic screening newborns inherited metabolic disorders only recently have variety new innovative applications begun to emerge. For many years, pre-analytical variables were inappropriately considered field even...
Extracellular vesicles (EVs) are involved in mediating intercellular communication processes. An important goal within the EV field is study of biodistribution EVs and identification their target cells. Considering that uptake assumed to be for processes, labeling with fluorescent dyes has emerged as a broadly distributed strategy cells tissues. However, accuracy specificity commonly utilized have not been sufficiently analyzed.By combining recent advances imaging flow cytometry phenotypic...
Cellular crosstalk between hematopoietic stem/progenitor cells and the bone marrow (BM) niche is vital for development maintenance of myeloid malignancies. These compartments can communicate via bidirectional transfer extracellular vesicles (EVs). EV trafficking in acute leukemia (AML) plays a crucial role shaping BM microenvironment into leukemia-permissive niche. Although several isolation methods have been developed, it remains major challenge to define most accurate reliable procedure....
Background aimsExtracellular vesicles (EVs) derived from human mesenchymal stromal cells (MSCs) show immunomodulatory activity in different assays both vitro and vivo. In previous work, the authors compared potential of independent MSC-EV preparations a multi-donor mixed lymphocyte reaction (mdMLR) assay an optimized steroid-refractory acute graft-versus-host disease (aGVHD) mouse model. The observed that only proportion showed capabilities demonstrated with mdMLR immunomodulating activities...
People who inject drugs are at high risk for hepatitis B, C and HIV. HTLV was reported by neighboring countries to be prevalent in this population, but the situation Germany is unclear. To generate seroprevalence related behavioural data enhance prevention efforts against these infections drug users Germany, a multicentre sero- survey initiated. not well reached services testing counselling blood-borne Germany. An interventional part of study intended prove feasibility acceptance...
Abstract Extracellular vesicles (EVs) are involved in mediating intercellular communication processes. An important goal within the EV field is study of biodistribution EVs and identification their target cells. Considering that uptake central for role processes, labelling with fluorescent dyes has emerged as a broadly distributed strategy cells tissues. However, accuracy specificity commonly utilized not been sufficiently analyzed. By combining recent advancements imaging flow cytometry...
Introduction Extracellular vesicles (EVs) are biological nanoparticles produced by virtually all cells. Depending on the cell of origin, these can carry different proteins their surface. This makes EVs a promising biomarker for health and disease, especially if they be obtained from plasma samples. However, preanalytics is crucial. The anticoagulants used blood collection processing steps required to prepare EV analysis could have significant impact diagnostic usefulness EVs. Another...