A5080667810- SARS-CoV-2 and COVID-19 Research
- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cardiac Valve Diseases and Treatments
- Blood groups and transfusion
- Acute Myeloid Leukemia Research
- Multiple Myeloma Research and Treatments
- Erythrocyte Function and Pathophysiology
- Bone fractures and treatments
- Platelet Disorders and Treatments
- Epigenetics and DNA Methylation
- Long-Term Effects of COVID-19
- Medical and Health Sciences Research
- Proteoglycans and glycosaminoglycans research
- Cardiac, Anesthesia and Surgical Outcomes
- Ethics in medical practice
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- Lymphoma Diagnosis and Treatment
Heinrich Heine University Düsseldorf
2016-2025
Düsseldorf University Hospital
2016-2025
Jena University Hospital
2023-2025
Friedrich Schiller University Jena
2024
University Hospital of Bern
2011-2024
Integrated Oncology (United States)
2024
Klinikum rechts der Isar
2023
University Hospital Leipzig
2023
Technical University of Munich
2023
CTI BioPharma (United Kingdom)
2003-2021
Here a new, intrinsically pluripotent, CD45-negative population from human cord blood, termed unrestricted somatic stem cells (USSCs) is described. This rare grows adherently and can be expanded to 1015 without losing pluripotency. In vitro USSCs showed homogeneous differentiation into osteoblasts, chondroblasts, adipocytes, hematopoietic neural including astrocytes neurons that express neurofilament, sodium channel protein, various neurotransmitter phenotypes. Stereotactic implantation of...
Significance The infrequent detection of circulating tumor cells (CTCs) has hindered their clinical implication and potential use in the sense a “liquid biopsy” for cancer diagnosis therapy. Hypothesizing that limited blood volume commonly used CTC analysis (1–10 mL) accounts variable rates, we leukapheresis to screen large volumes CTCs. This enabled more reliable CTCs at high frequency even nonmetastatic patients. Thus, diagnostic may facilitate routine as biomarkers personalized medicine....
Abstract Despite scientific evidence originating from two patients published to date that CCR5Δ32/Δ32 hematopoietic stem cell transplantation (HSCT) can cure human immunodeficiency virus type 1 (HIV-1), the knowledge of immunological and virological correlates is limited. Here we characterize a case long-term HIV-1 remission 53-year-old male who was carefully monitored for more than 9 years after allogeneic HSCT performed acute myeloid leukemia. sporadic traces DNA detected by droplet...
Abstract Although autologous bone grafting represents an effective tool to induce osteogenic regeneration in local defects or pseudarthroses, it is associated with significant donor site morbidity and limited by the amount available for grafting. We investigate potency of marrow aspiration concentrate (BMAC) augment support healing. The functional radiographic outcome 39 patients volumetric deficiencies treated BMAC are presented evaluated a prospective clinical trial. A collagen sponge...
The development of a fatal, clonal, autonomously proliferating CD4-CD8- chimeric antigen receptor (CAR)+ peripheral T-cell lymphoma (PTCL) occurred 1 month after patient received treatment with tisagenlecleucel for relapsed primary central nervous system lymphoma. PTCL had clonal rearrangement, which was already detectable in the apheresis product CAR manufacturing and 7 months earlier autologous transplantation. Somatic
Autosomal recessive congenital ichthyosis (ARCI) represents a heterogeneous group of rare disorders cornification with 3 major subtypes: harlequin (HI), lamellar (LI) and ichthyosiform erythroderma (CIE). A 4th subtype has also been proposed: pleomorphic (PI), characterized by marked skin changes at birth subsequently mild symptoms. In nationwide screenings suspected cases ARCI in Denmark Sweden, we identified 132 patients (age range 0.1–86 years) classified as HI (n=7), LI (n=70), CIE...
Frequently, the number of circulating tumor cells (CTC) isolated in 7.5 mL blood is too small to reliably determine heterogeneity and be representative as a “liquid biopsy”. In EU FP7 program CTCTrap, we aimed validate optimize recently introduced Diagnostic LeukApheresis (DLA) screen liters blood. Here present results obtained from 34 metastatic cancer patients subjected DLA participating institutions. About processed with CellSearch® was used “gold standard” reference. DLAs were 22...
Circulating tumor cells (CTCs) are promising surrogate markers for systemic disease, and their molecular characterization might be relevant to guide more individualized cancer therapies. To enable fast efficient purification of individual CTCs, we developed a work flow from CellSearch(TM) cartridges enabling high-resolution genomic profiling on the single-cell level.Single CTCs were sorted 40 CellSearch samples patients with metastatic breast using MoFlo XDP cell sorter. Genomes single...
Abstract Diagnostic leukapheresis (DLA) is based on continuous centrifugation that collects mononuclear cells from peripheral blood with a density of 1.055–1.08 g/ml. As epithelial have similar density, DLA cocollects circulating tumor cell (CTCs) along the targeted cells. Here, we report our single center experience applying in 40 nonmetastatic and metastatic breast cancer patients its impact CTC detection. We found use just 5% product (corresponding to median volume around 60 ml)...
Stem cell and tissue engineering-based therapies have become a promising option to heal bony defects in the future. Human cord blood-derived mesenchymal stem cells were seeded onto collagen/tricalcium phosphate scaffold xenotransplanted into critical size femoral of 46 nude rats. We found survival human within surrounding bone/bone marrow up 4 wk after transplantation an increased bone healing rate compared with controls without cells. This study supports application blood for...
Abstract Killer Ig-like receptors (KIR) and HLA class I ligands were studied in unrelated hemopoietic stem cell transplantation for chronic myeloid leukemia (n = 108). Significantly improved overall survival was observed patients, which homozygous HLA-C-encoded group 1 (C1) compared with those 2 (C2) ligands. Favorable outcome the former patient an early effect that highly significant patients transplanted G-CSF-mobilized peripheral blood advanced disease stages. In contrast, presence of C1...
In addition to osteosynthetic stabilizing techniques and autologous bone transplantations, so-called orthobiologics play an increasing role in the treatment of healing disorders. Besides use various growth factors, more new data suggest that cell-based therapies promote local regeneration. For ethical biological reasons, clinical application progenitor cells on musculoskeletal system is limited autologous, postpartum stem cells. Intraoperative one-step with cells, particular, delivered...