A5020192294- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Thyroid Cancer Diagnosis and Treatment
- Eosinophilic Esophagitis
- Single-cell and spatial transcriptomics
- Microfluidic and Bio-sensing Technologies
- Carbon and Quantum Dots Applications
- Pancreatic and Hepatic Oncology Research
- Nanocluster Synthesis and Applications
- Cell death mechanisms and regulation
- Epigenetics and DNA Methylation
- Cytokine Signaling Pathways and Interactions
- Neuroendocrine Tumor Research Advances
- Graphene and Nanomaterials Applications
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Inflammatory Bowel Disease
- Immune cells in cancer
- Cancer Research and Treatments
- Calcium signaling and nucleotide metabolism
- MicroRNA in disease regulation
- Testicular diseases and treatments
- Thyroid Disorders and Treatments
Düsseldorf University Hospital
2012-2025
Heinrich Heine University Düsseldorf
2015-2025
CTI BioPharma (United Kingdom)
2013-2020
Cellular Therapeutics (United Kingdom)
2013-2014
Significance The infrequent detection of circulating tumor cells (CTCs) has hindered their clinical implication and potential use in the sense a “liquid biopsy” for cancer diagnosis therapy. Hypothesizing that limited blood volume commonly used CTC analysis (1–10 mL) accounts variable rates, we leukapheresis to screen large volumes CTCs. This enabled more reliable CTCs at high frequency even nonmetastatic patients. Thus, diagnostic may facilitate routine as biomarkers personalized medicine....
Circulating tumor cells (CTCs) are promising surrogate markers for systemic disease, and their molecular characterization might be relevant to guide more individualized cancer therapies. To enable fast efficient purification of individual CTCs, we developed a work flow from CellSearch(TM) cartridges enabling high-resolution genomic profiling on the single-cell level.Single CTCs were sorted 40 CellSearch samples patients with metastatic breast using MoFlo XDP cell sorter. Genomes single...
To determine the role of BRAF V600E mutation and MAPK signaling as well effects MEK directed therapy in gastroenteropancreatic neuroendocrine neoplasia (GEP‐NEN), with a focus on highly aggressive carcinoma (GEP‐NEC). Using Sanger sequencing exon 15 we determined frequency mutations 71 primary GEP‐NENs. phosphorylation was examined by immunohistochemistry corresponding tissue samples. evaluate biological relevance GEP‐NECs, pharmacological inhibition were analyzed NEC cell lines both vitro...
Comprehensive genome wide analyses of single cells became increasingly important in cancer research, but remain to be a technically challenging task. Here, we provide protocol for array comparative genomic hybridization (aCGH) cells. The is based on an established adapter-linker PCR (WGAM) and allowed us detect copy number alterations as small 56 kb In addition report factors influencing the success cell aCGH downstream amplification method, including characteristics reference DNA, labeling...
Human leukocytes obtained from samples of leukapheresis products three healthy donors stimulated by granulocyte colony stimulating factor (G-CSF) were exposed to graphene quantum dots.
Abstract Among various nanoparticles tested for pharmacological applications over the recent years, graphene quantum dots (GQDs) seem to be promising candidates construction of drug delivery systems due their superior biophysical and biochemical properties. The subcellular fate incorporated nanomaterial is decisive transporting pharmaceuticals into target cells. Therefore a detailed characterization uptake GQDs different breast cancer models was performed. demonstrated accumulation inside...
Despite their identification several years ago, molecular identity and developmental relation between human ILC1 NK cells, comprising group 1 ILCs, is still elusive. To unravel connection, thorough transcriptional, epigenetic, functional characterization was performed from umbilical cord blood (CB). Unexpectedly, ILC1-like cells lacked Tbet expression failed to produce IFNγ. Moreover, in contrast previously described subsets they could be efficiently differentiated into cells. These were...
Background: Follicular thyroid carcinoma's (FTC) often benign course is partially due to adjuvant radioactive iodine (RAI) treatment. However, once the tumour has spread and fails retain RAI, therapeutic options are limited outcome poor. In this subset of patients, identification novel druggable biomarkers appears invaluable. Here, we investigated stage dependent expression functional role C-X-C chemokine receptors type 4 7 (CXCR4/7) in FTC. Methods: CXCR4/7 was examined 44 FTC corresponding...
Medullary thyroid carcinoma (MTC) is a rare and challenging endocrine malignancy. Once spread, the therapeutic options are limited outcome poor. For these patients, identification of new druggable biological markers great importance. Here, we investigated prognostic role C-X-C chemokine receptors type 4 7 (CXCR4/7) in MTC. Eighty-six MTC corresponding non-neoplastic specimens were immunohistochemically stained for CXCR4/7 using tissue microarray technology expression levels correlated with...
Here, we tested two magnetic-bead based systems for the enrichment and detection of rare tumor cells in concentrated blood products. For that, defined numbers from three pancreatic cancer cell lines were spiked 10
Abstract Innate lymphoid cells (ILCs), comprising ILC1, 2, and 3 subpopulations, play unique roles in maintaining microbiome homeostasis, mucosal tissue integrity, control of inflammation. So far, their characterization is dominantly based on tissue-resident ILCs, whereas little information available circulating particular newborns. In order to get a deeper understanding neonatal innate immunity, we analyzed the transcriptomes effector functions cord blood (CB) ILCs. By RNAseq analysis, all...
Abstract Relapsing germ cell tumor (GCT) patients often harbor components of the aggressive subtype yolk‐sac (YST), suggesting that YST formation is an escape mechanism under therapy. Nevertheless, molecular mechanisms inducing development from its stem cell‐like precursor embryonal carcinoma (EC) are largely unexplored. We demonstrated induction transcription factor SOX17 together with stimulation WNT, TGF‐beta / Activin, and FGF signaling drives EC cells into lineage. Single RNA sequencing...
Introduction: Hematopoietic stem and progenitor cells (HSPCs) from umbilical cord blood (CB) are an established source of treatment for hematologic disorders developing cell therapies. However, pre-clinical research on B NK development relies traditional murine stromal cell-based systems with reduced physiological relevance clinical applicability. Methods: A serum-free, fully humanized co-culture system utilizing human bone marrow-derived mesenchymal (BM-MSCs) was developed to differentiate...
Abstract Gene expression studies of peripheral blood cells in inflammatory diseases revealed a large array new antigens as potential biomarkers useful for diagnosis, prognosis, and therapy stratification. Generally, their validation on the protein level remains mainly restricted to more hypothesis‐driven manner. State‐of‐the‐art multicolor flow cytometry make it attractive validate candidate genes at single cell combined with detailed immunophenotyping subsets. We developed staining panels...
Abstract Follicular thyroid carcinoma’s (FTC) overall good prognosis deteriorates if the tumour fails to retain radioactive iodine. Therefore, new druggable targets are in high demand for this subset of patients. Here, we investigated prognostic and biological role survivin XIAP FTC. Survivin expression was 44 FTC corresponding non-neoplastic specimens using tissue microarrays. Inhibition both inhibitor apoptosis proteins (IAP) induced by shRNAs or specific small molecule antagonists...
Gastroenteropancreatic neuroendocrine neoplasms (GEP-NEN) represent a rare and heterogenous tumor entity. Importantly, the highly proliferative subgroup of carcinoma (GEP-NEC) is characterized by high resistance to conventional chemotherapy. Consequently, there an urgent need identify novel therapeutic targets, especially for GEP-NEC. Thus, we focused on Inhibitor apoptosis protein (IAP) family members survivin XIAP that orchestrate inhibition apoptosis, induce against chemotherapeutics...
Abstract Inflammation after injury of the central nervous system (CNS) is increasingly viewed as a therapeutic target. However, comparative studies in different CNS compartments are sparse. To date only few based on immunohistochemical data and all referring to mechanical have directly compared inflammation compartments. These revealed that more pronounced spinal cord than brain. Therefore, it unclear whether concepts treatments established cerebral cortex can be transferred lesions vice...
The tumor microenvironment (TM), consisting of the extracellular matrix (ECM), fibroblasts, endothelial cells, and immune might affect invasiveness outcome standard chemotherapy. This study investigated cross talk between germ cell tumors (GCT) surrounding TM cells (macrophages, T-lymphocytes, fibroblasts) at transcriptome secretome level. Using high-throughput approaches three-dimensional (3D) co-cultured cellular aggregates, this offers newly identified pathways to be studied with regard...
Follicular thyroid cancer's (FTC) excellent long-term prognosis is mainly dependent on postoperative radioactive iodine (RAI) treatment. However, once the tumour becomes refractory, 10-year disease-specific survival rate drops below 10%. The aim of our study was to evaluate prognostic and biological role TRAIL system in FTC elucidate influence small-molecule-mediated antagonisation inhibitor apoptosis proteins (IAPs) sensitivity vitro Tissue microarrays were constructed from forty-four...
Abstract Diagnostic leukapheresis (DLA) enables to sample larger blood volumes and increases the detection of circulating tumor cells (CTC) significantly. Nevertheless, high excess white (WBC) DLA products remains a major challenge for further downstream CTC enrichment detection. To address this problem, we tested performance two label-free technologies processing products. For testing purposes, established ficollized buffy coats (BC) with WBC composition similar patient-derived The...
Innate lymphoid cells (ILCs) and in particular ILC3s have been described to be vital for mucosal barrier functions homeostasis within the gastrointestinal (GI) tract. Importantly, IL-22-secreting ILC3 implicated control of inflammatory bowel disease (IBD) were shown reduce incidence graft-versus-host (GvHD) as well risk transplant rejection. Unfortunately, are primarily located tissues not found circulation, making access them humans challenging. On this account, there is a growing desire...
Cladribine is a synthetic purine analogue that interferes with DNA synthesis and repair next to disrupting cellular proliferation in actively dividing lymphocytes. The compound approved for the treatment of multiple sclerosis (MS). can cross blood-brain barrier, suggesting potential effect on central nervous system (CNS) resident cells. Here, we explored compartment-specific immunosuppressive as well direct neuroprotective effects oral cladribine experimental autoimmune encephalomyelitis...