A5060042575- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Hepatocellular Carcinoma Treatment and Prognosis
- Advanced Breast Cancer Therapies
- HER2/EGFR in Cancer Research
- Cancer Immunotherapy and Biomarkers
- MicroRNA in disease regulation
- Cancer Treatment and Pharmacology
- DNA and Nucleic Acid Chemistry
- Liver Disease Diagnosis and Treatment
- Ferroptosis and cancer prognosis
- Metastasis and carcinoma case studies
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Advanced biosensing and bioanalysis techniques
- Cancer, Hypoxia, and Metabolism
- Breast Cancer Treatment Studies
- Renal cell carcinoma treatment
- Cancer-related Molecular Pathways
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer-related molecular mechanisms research
- Nanoplatforms for cancer theranostics
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
LMU Klinikum
2018-2025
Ludwig-Maximilians-Universität München
2015-2025
München Klinik
2016-2024
University of Twente
2018
Klinik für Frauenheilkunde
2016-2018
Universität Hamburg
2016-2017
Heidelberg University
1999-2017
University Hospital Heidelberg
2016-2017
University Medical Center Hamburg-Eppendorf
2016-2017
University Hospital Ulm
2016-2017
In this study we investigate the mechanism of intracellular pH change and its role in malignant transformation using E7 oncogene human papillomavirus type 16 (HPV16). Infecting NIH3T3 cells with recombinant retroviruses expressing HPV16 or a deficient mutant show that alkalinization is specific. which can be turned on followed by induction expression, demonstrate cytoplasmic an early event was driven stimulation Na + /H exchanger activity via increase affinity NHE‐1 proton regulatory site....
The Parkinson's disease (PD)-associated gene DJ-1 mediates direct neuroprotection. up-regulation of in reactive astrocytes also suggests a role glia. Here we show that regulates proinflammatory responses mouse astrocyte-rich primary cultures. When treated with Toll-like receptor 4 agonist, the bacterial endotoxin lipopolysaccharide (LPS), Dj-1-knockout generated >10 times more nitric oxide (NO) than littermate controls. Lentiviral reintroduction restored NO response to LPS. enhanced...
The prognostic relevance of circulating tumor cells (CTCs) at the time primary diagnosis has been well established. However, little information is available regarding their to follow-up care.The multicenter, open-label, phase III SUCCESS A trial compared two adjuvant chemotherapy regimens followed by 2 vs 5 years zoledronate for early-stage, high-risk breast cancer patients. presence CTCs was assessed before and after using FDA-approved CellSearch System. Overall survival (OS) disease-free...
The presence of high expressing epithelial cell adhesion molecule (EpCAMhigh) circulating tumor cells (CTC) enumerated by CellSearch® in blood cancer patients is strongly associated with poor prognosis. This raises the question about and relation clinical outcome low EpCAM CTC (EpCAMlow CTC). In EU-FP7 CTC-Trap program, we investigated EpCAMhigh EpCAMlow using CellSearch, followed microfiltration depleted blood. Blood samples 108 castration-resistant prostate 22 metastatic breast were...
Abstract Background Liquid biopsy based on cell-free DNA circulating in plasma has shown solid results as a non-invasive biomarker. In the present study we evaluated utility of free (cfDNA) and sub-type tumor (ctDNA) hepatocellular cancer (HCC) patients to assess therapy response clinical outcome. Methods A cohort 13 recruited context SORAMIC trial with unresectable, advanced HCC different etiological clinicopathological characteristics was included this exploratory study. Plasma samples...
Abstract Purpose: SORAMIC is a randomized controlled trial in patients with advanced hepatocellular carcinoma (HCC) undergoing sorafenib ± selective internal radiation therapy (SIRT). We investigated the value of extracellular vesicle (EV)-based proteomics for treatment response prediction. Experimental Design: The analysis population comprised 25 receiving SIRT+sorafenib and 20 alone. Patients were classified as responders or nonresponders based on changes AFP imaging overall survival....
In recent years, the field of epigenetics has grown dramatically and become one most dynamic fast-growing branches molecular biology. The amount diseases suspected being influenced by DNA methylation is rising steadily includes common such as schizophrenia, bipolar disorder, Alzheimer's disease, diabetes, atherosclerosis, cancer, major psychosis, lupus Parkinson's disease. Due to cellular heterogeneity patterns, epigenetic analyses single cells a necessity. One rationale that profiles are...
Monitoring treatment and early detection of fatal breast cancer (BC) remains a major unmet need. Aberrant circulating DNA methylation (DNAme) patterns are likely to provide highly specific signal. We hypothesized that cell-free DNAme markers could indicate disseminated cancer, even in the presence substantial quantities background DNA. used reduced representation bisulfite sequencing (RRBS) 31 tissues established serum assays based on ultra-high coverage two independent prospective sets (n =...
Circulating tumor cells (CTCs) isolated from blood can be probed for the expression of treatment targets. Immunofluorescence is often used both enumeration CTC and determination protein levels related to Accurate reproducible assessment such target essential their use in clinic. To enable this, an open source image analysis program named ACCEPT was developed EU-FP7 CTCTrap CANCER-ID programs. Here its application shown on a retrospective cohort 132 metastatic breast cancer patients which...
microRNAs (miRNAs) are considered promising cancer biomarkers, showing high reliability, sensitivity and stability. Our study aimed to identify associations between whole blood miRNA profiles, presence of circulating tumor cells (CTCs) clinical outcome in post-operative early breast patients (EBC) assess the utility miRNAs as prognostic markers this setting. A total 48 patients, recruited frame SUCCESS trial, were included retrospective tested with a panel 8 (miR-10b, −19a, − 21, 22, −20a,...
Cell proliferation and terminal differentiation are mutually exclusive in most cell lineages. The b-zip transcription factor CCAAT/enhancer-binding protein α (C/EBPα) induces arrest many types, suggesting that both activities linked. Here we show C/EBPα-mediated pathways can be separated by the E7 oncoprotein of “high-risk” human papilloma virus 16. overrides cycle withdrawal without compromising transactivation activity C/EBPα or its ability to participate differentiation. Uncoupling...
Introduction: Immunomodulating effects of Helicobacter pylori (H. pylori) have been shown to inhibit antitumor immunity. Resistance immune checkpoint inhibitor (ICI)-based therapies is common among patients with hepatocellular carcinoma (HCC). This study aimed assess the effect H. on outcomes ICI in HCC. Methods: We conducted a multicenter HCC across broad range treatments. Patients received either ICI-based combination regimens or sorafenib-based therapy. serostatus and virulence factors...
Hepatocellular carcinoma is frequently unrecognized in its early stage limiting the access to first therapeutic steps resulting a low cure rate. Therefore, an diagnosis crucial. In this scenario analysis of lipidome and metabolome emerged as promising tool for detection. Aim study was characterize metabolomic profiles novel markers hepatocellular carcinoma. Serum basal levels metabolites, isolated from cohort 90 patients (n = 30 stage; n advanced liver cirrhosis) were analysed using nuclear...